{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:21:57Z","timestamp":1790716917515,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Research in the past decade has elucidated the explicit role of the immune system in the pathophysiology of osteoporosis. Recent studies have further unraveled the complex interactions between bone and immune cells and explored safe, effective immunomodulatory approaches\u2014such as probiotics\u2014for preventing and managing osteoporosis. As a result, various immune factors have continuously been discovered to play specific roles in maintaining bone homeostasis. The role of Tregs in the context of postmenopausal osteoporosis (PMO) is already well established. While Foxp3\n                    <jats:sup>+<\/jats:sup>\n                    Tregs are mostly matured in the thymus (tTregs), some are also produced from Foxp3\n                    <jats:sup>\u2212<\/jats:sup>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell precursors in the peripheral tissues (i.e., pTregs). Notably, the specific role of pTregs and tTregs in PMO remains to be elucidated. Here, we reveal that estrogen\u2010deficient inflammatory conditions in PMO disrupt the balance of tTregs and pTregs. Interestingly, within pTregs, the population of ROR\u03b3T\n                    <jats:sup>\u2212<\/jats:sup>\n                    pTregs and ROR\u03b3T\n                    <jats:sup>+<\/jats:sup>\n                    pTregs is further altered, along with simultaneous expansion of Th17 cells\u2014likely through the conversion of ROR\u03b3T\n                    <jats:sup>\u2212<\/jats:sup>\n                    pTregs into Th17 cells. Notably, supplementation with\n                    <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                    (LA) restores the homeostasis of ROR\u03b3T\n                    <jats:sup>\u2212<\/jats:sup>\n                    pTregs and Th17 cells in a butyrate\u2010mediated manner. Moreover, it was observed that butyrate\u2010primed ROR\u03b3T\n                    <jats:sup>\u2212<\/jats:sup>\n                    pTregs have reduced osteoclastogenic potential. Collectively, our findings for the first time reveal the pivotal role of gut resident ROR\u03b3T\n                    <jats:sup>\u2212<\/jats:sup>\n                    pTregs\u2013Th17 cell axis in the pathophysiology of PMO.\n                  <\/jats:p>","DOI":"10.1002\/jcp.70114","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T08:03:47Z","timestamp":1764835427000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Lactobacillus acidophilus<\/i>\n                    Ameliorates Inflammatory Bone Loss Under Postmenopausal Osteoporotic Conditions via Modulating the Gut\u2010Resident ROR\u03b3T\n                    <sup>\u2212<\/sup>\n                    pTreg Cell Population"],"prefix":"10.1002","volume":"240","author":[{"given":"Asha","family":"Bhardwaj","sequence":"first","affiliation":[{"name":"Translational Immunology, Osteoimmunology &amp; Immunoporosis Lab (TIOIL), An ICMR Collaborating Centre for Excellence on Bone Health, Department of Biotechnology All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leena","family":"Sapra","sequence":"additional","affiliation":[{"name":"Translational Immunology, Osteoimmunology &amp; Immunoporosis Lab (TIOIL), An ICMR Collaborating Centre for Excellence on Bone Health, Department of Biotechnology All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3609-6628","authenticated-orcid":false,"given":"Chaman","family":"Saini","sequence":"additional","affiliation":[{"name":"Translational Immunology, Osteoimmunology &amp; Immunoporosis Lab (TIOIL), An ICMR Collaborating Centre for Excellence on Bone Health, Department of Biotechnology All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9408-7917","authenticated-orcid":false,"given":"Abhay","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Centre for Rural Development &amp; Technology Indian Institute of Technology (IIT) New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aishwarya","family":"Nilakhe","sequence":"additional","affiliation":[{"name":"Molecular Science Lab National Institute of Immunology (NII) New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0795-2819","authenticated-orcid":false,"given":"Pradyumna K.","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology ICMR\u2010National Institute for Research in Environmental Health Bhopal Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3323-0713","authenticated-orcid":false,"given":"Rupesh K.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Translational Immunology, Osteoimmunology &amp; Immunoporosis Lab (TIOIL), An ICMR Collaborating Centre for Excellence on Bone Health, Department of Biotechnology All India Institute of Medical Sciences (AIIMS) New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jleuko\/qiaf008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2100175"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-016-3712-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2018.03.059"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2018.02.013"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bonr.2018.02.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20896-y"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4322\/acr.2020.186"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25384-x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15248-w"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2022.10.009"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.04.042"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3432"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-017-0691-z"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI86062"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2010.54"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.066"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.1003712"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-042718-041717"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40011-021-01238-x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-80536-2"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2025.2492378"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.875788"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202049123"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201847498"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00657"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S351918"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.36"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00160"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23138"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.70114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T07:00:17Z","timestamp":1767078017000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.70114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/jcp.70114"],"URL":"https:\/\/doi.org\/10.1002\/jcp.70114","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-05-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70114"}}