{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T08:09:43Z","timestamp":1776240583141,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    O\u2010GlcNAcylation is a post\u2010translational modification involved in various cellular processes, including cell cycle progression, signaling, transcription, and stress response. Mouse salivary gland morphogenesis shows specific localization patterns of O\u2010GlcNAc transferase (OGT) and O\u2010GlcNAc in developing acinar cells, suggesting a potential involvement of O\u2010GlcNAcylation in acinar cell differentiation\u2010related signaling molecules. To define its underlying mechanisms, this study used an OGT inhibitor, OSMI\u20101, and small interfering RNA (siRNA) targeting OGT, during\n                    <jats:italic>in vitro<\/jats:italic>\n                    cultivation of submandibular glands and assessed morphological and molecular alterations using histology, immunohistochemistry, Western blot, and RT\u2010qPCR. As expected, OGT inhibition impaired terminal bud morphogenesis and altered cellular physiology. OSMI\u20101 treatment disrupted acinar cell differentiation, reflected by changes in expression patterns of signaling molecules crucial to acinar cell differentiation, including Sox9, Sox10, E\u2010cadherin, and Mist1. Altered expression patterns of cytokeratins, including CK14 and CK18, confirmed altered ductal morphology. Therefore, our findings highlight the essential role of OGT\u2010mediated O\u2010GlcNAcylation in salivary gland morphogenesis with post\u2010translational regulation of key signaling molecules governing functional differentiation of acinar cells.\n                  <\/jats:p>","DOI":"10.1002\/jcp.70126","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T07:41:24Z","timestamp":1768808484000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulating O\u2010GlcNAcylation Alters Salivary Acinar Cell Differentiation"],"prefix":"10.1002","volume":"241","author":[{"given":"Elina","family":"Pokharel","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR Kyungpook National University Daegu South 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