{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T08:10:02Z","timestamp":1776240602700,"version":"3.50.1"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to determine whether degeneration of intervertebral discs and subchondral bone correlates with the development of persistent low back pain (LBP) in a rat model with intradiscal monosodium iodoacetate (MIA), focusing on structural alterations, neuronal sensitization, and pain\u2010related behavior. Male Sprague Dawley rats received MIA injections (2\u2009mg in 2\u2009\u03bcl) into L4\/5 and L5\/6 lumbar discs to induce degeneration. LBP\u2010related behaviors were assessed over 42 days via dynamic weight\u2010bearing and hindpaw withdrawal thresholds. Neuronal sensitization was evaluated via electrophysiological recordings of mechanosensitive afferent nerves (MSAN) and calcium imaging of labeled dorsal root ganglia (DRG) neurons innervating discs. Structural alterations in subchondral bone and disc were analyzed using in vivo\/ex vivo \u00b5CT imaging, along with immunohistochemistry for TRPV1 and CGRP expression. Intradiscal MIA induced long\u2010term LBP behaviors, characterized by forelimb weight\u2010bearing shifts and reduced hindpaw withdrawal thresholds. MSAN exhibited hyperexcitability and lowered firing thresholds to intradiscal pressure, while DRG neurons showed enhanced TRPV1\u2010 and TRPA1\u2010mediated calcium influx. \u00b5CT imaging revealed decreased disc volume and deteriorated trabecular bone quality. Histology confirmed disc and subchondral bone degeneration, with upregulated TRPV1 and CGRP expression in subchondral bone, indicating nociceptive fiber ingrowth. Linear discriminant analysis identified weight\u2010bearing asymmetry (HindFore AUC), trabecular thickness, connectivity, and trabecular pattern factor as strong predictors of LBP development. Pathological degeneration of disc and bone is associated with chronic LBP through structural remodeling and peripheral neuronal sensitization. These findings suggest that therapeutic strategies targeting both tissues can alleviate chronic pain in degenerative spine conditions.<\/jats:p>","DOI":"10.1002\/jcp.70140","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T05:55:37Z","timestamp":1769147737000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathological Degeneration of Disc and Bone Is Associated With Chronic Low Back Pain in a Rat Model With Intradiscal Monosodium Iodoacetate"],"prefix":"10.1002","volume":"241","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6790-0308","authenticated-orcid":false,"given":"Yun\u2010Ho","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Physiology, College of Medicine and Neuroscience Research Institute Korea University Seoul South 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