{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:19:14Z","timestamp":1787757554399,"version":"build-2784847793"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82572480"],"award-info":[{"award-number":["82572480"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2025SYS\u2010SYSZD\u2010087"],"award-info":[{"award-number":["2025SYS\u2010SYSZD\u2010087"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sepsis, characterized by a rapid transition to systemic immune dysregulation and multiorgan failure, poses a formidable clinical challenge. The lack of spatiotemporally stable biomarkers severely impedes early diagnosis and risk stratification. By integrating large\u2010scale transcriptomic profiling with machine learning algorithms, this study identified a robust three\u2010gene diagnostic signature (TLR5, HMGB2, and C19orf59). Single\u2010cell RNA sequencing precisely localized the sepsis\u2010induced specific upregulation of these targets to the myeloid immune compartment, notably monocytes and neutrophils. Crucially, disease severity stratification analysis (based on SOFA scores) revealed that while TLR5 and HMGB2 excel in identifying high\u2010risk sepsis, C19orf59 maintains highly consistent diagnostic efficacy across all clinical severity strata. To definitively validate these findings while rigorously eliminating confounding effects from surgical or environmental stress, strictly time\u2010matched sham\u2010controlled cecal ligation and puncture (CLP) murine models and vehicle\u2010controlled\n                    <jats:italic>in vitro<\/jats:italic>\n                    models were utilized.\n                    <jats:italic>In vivo<\/jats:italic>\n                    results corroborated the persistent in situ upregulation of this signature across vital target organs (lung, heart, liver) and systemic circulation. Parallel\n                    <jats:italic>in vitro<\/jats:italic>\n                    lipopolysaccharide (LPS)\u2010stimulated cellular models further characterized their dynamic expression, with HMGB2 exhibiting a distinct biphasic kinetic profile mechanistically characteristic of danger\u2010associated molecular patterns (DAMPs). Finally, independent clinical validation using sepsis patient serum corroborated the translational relevance of these targets. Collectively, this systematic multidimensional evaluation establishes TLR5, HMGB2, and C19orf59 as a highly reliable diagnostic and severity\u2010stratification panel, providing novel molecular insights into the septic pathological cascade.\n                  <\/jats:p>","DOI":"10.1002\/jcp.70213","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:18:33Z","timestamp":1785410313000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multiomics Profiling Identifies Blood\u2010Based Diagnostic Markers for Sepsis"],"prefix":"10.1002","volume":"241","author":[{"given":"Xin","family":"Zhang","sequence":"first","affiliation":[{"name":"Xi'an Key Laboratory of Innovative Drug Research for Heart Failure, Faculty of Life Sciences and Medicine Northwest University Xi'an 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Innovative Drug Research for Heart Failure, Faculty of Life Sciences and Medicine Northwest University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xi'an People's Hospital (Xi'an Fourth Hospital) The Affiliated People's Hospital of Northwest University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Chang","sequence":"additional","affiliation":[{"name":"Xi'an Key Laboratory of Innovative Drug Research for Heart Failure, Faculty of Life Sciences and Medicine Northwest University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1163-2359","authenticated-orcid":false,"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Xi'an Key Laboratory of Innovative Drug Research for Heart Failure, Faculty of Life Sciences and Medicine Northwest 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