{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:19:15Z","timestamp":1787757555984,"version":"build-2784847793"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","award":["R016720003"],"award-info":[{"award-number":["R016720003"]}],"id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cancer\u2010associated fibroblasts (CAFs) are key drivers of tumor progression. This study examined how three\u2010dimensional (3D) culture, hypoxia, and cancer\u2010derived soluble factors influence the transformation of human mesenchymal stem cells (hMSCs) into inflammatory CAFs (iCAFs). hMSCs from bone marrow, placenta, and chorion were cultured in 2D, in Matrigel\u2010based 3D systems, under hypoxia, and with soluble factors from colon cancer cells (HT29, HCT116). 3D culture strongly induced iCAF markers (IL1\u03b1, CSF3, IL6) while reducing myofibroblastic CAF markers (CCN2, MYL9, TAGLN). Hypoxia and cancer factors further enhanced this phenotype, promoting IL1\u03b1\/IL6 secretion and shifting their influence from suppressing to stimulating cancer cell growth and angiogenesis. Mechanistically, these changes were associated with YAP\/TAZ down\u2010regulation, and genetic depletion of YAP\/TAZ alone was sufficient to convert hMSCs into iCAFs even in 2D culture. These findings highlight YAP\/TAZ as critical regulators of hMSC\u2010to\u2010CAF transformation, with implications for therapeutic strategies targeting tumor stroma.<\/jats:p>","DOI":"10.1002\/jcp.70219","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:58:33Z","timestamp":1785934713000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hippo Pathway Regulation and Microenvironmental Cues Governing Human MSC Transition to Cancer\u2010Associated Fibroblasts"],"prefix":"10.1002","volume":"241","author":[{"given":"Pimjai","family":"Chingsuwanrote","sequence":"first","affiliation":[{"name":"Department of Medicine, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2206-7065","authenticated-orcid":false,"given":"Chanchao","family":"Lorthongpanich","sequence":"additional","affiliation":[{"name":"Department of Medicine, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"},{"name":"Mechanobiology and Stem Cell Fate Research Group, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pakpoom","family":"Kheolamai","sequence":"additional","affiliation":[{"name":"Center of Excellence in Stem Cell Research and Innovation, Faculty of Medicine Thammasat University Pathumthani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting Gang","family":"Chew","sequence":"additional","affiliation":[{"name":"Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine Zhejiang University Hangzhou China"},{"name":"The Zhejiang University\u2010University of Edinburgh Institute, Zhejiang University School of Medicine Zhejiang University Haining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5912-6642","authenticated-orcid":false,"given":"Chuti","family":"Laowtammathron","sequence":"additional","affiliation":[{"name":"Department of Medicine, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1639-4935","authenticated-orcid":false,"given":"Sudjit","family":"Luanpitpong","sequence":"additional","affiliation":[{"name":"Department of Medicine, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"},{"name":"Mechanobiology and Stem Cell Fate Research Group, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4764-9101","authenticated-orcid":false,"given":"Rangsun","family":"Parnpai","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Institute of Agricultural Technology, Embryo Technology and Stem Cell Research Center Suranaree University of Technology Nakhon Ratchasima Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surapol","family":"Issaragrisil","sequence":"additional","affiliation":[{"name":"Department of Medicine, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand"},{"name":"Bangkok Hematology Center, Wattanosoth Hospital BDMS Center of Excellence for Cancer Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.12.112"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-024-03183-1"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-2586"},{"issue":"5","key":"e_1_2_12_5_1","first-page":"1751","article-title":"Heterogeneity of Malignant Cells From a Human Colonic Carcinoma","volume":"41","author":"Brattain M. 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