{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:56:04Z","timestamp":1790574964587,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00c7\u00c3o de Amparo \u00c0 Pesquisa do Estado de S\u00c3o Paulo","doi-asserted-by":"publisher","award":["2018\/07782\u20108"],"award-info":[{"award-number":["2018\/07782\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00c7\u00c3o de Amparo \u00c0 Pesquisa do Estado de S\u00c3o Paulo","doi-asserted-by":"publisher","award":["2020\/05117\u20107"],"award-info":[{"award-number":["2020\/05117\u20107"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00c7\u00c3o de Amparo \u00c0 Pesquisa do Estado de S\u00c3o Paulo","doi-asserted-by":"publisher","award":["2018\/05064\u20100"],"award-info":[{"award-number":["2018\/05064\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00c7\u00c3o de Aperfei\u00c7oamento de Pessoal de N\u00cdvel Superior","doi-asserted-by":"publisher","award":["C\u00d3digo de Financiamento 001"],"award-info":[{"award-number":["C\u00d3digo de Financiamento 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gastric gland cells originate from two proliferative niches: the main rapid one in the isthmus, and the slow secondary one in the base. However, while nutrition is dependent on breastfeeding, the proliferative niches are not established, and proliferation occurs along the gland. Currently, we investigated the influence of early weaning (EW) on shaping gastric proliferative niches and their signaling. Wistar rats were separated into suckling (S) and EW groups on postnatal day (PND) 15 for gastric mucosa collection. BrdU was administered (PND 14 and 15) and as it diluted between daughter\u2010cells we evaluated migration and differentiation. In parallel EdU incorporation was used to identify cells during S phase. Through these tools, we studied the different profiles of BrdU\/EdU dual labeling at 18, 21 and 30 PND. At 18 PND, EW decreased BrdU+ population (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001\n                    <jats:italic>vs<\/jats:italic>\n                    . S group) in the upper and bottom gland areas (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The BrdU\u2009+\u2009\/EdU+ cells were concentrated in the upper gland, where we recorded a larger EdU+ population after EW (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). On PND 30, EW decreased BrdU+ cells in the gland (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), whereas EdU+ cells were more numerous in the isthmus. At 18 PND, EW reduced\n                    <jats:italic>Wnt3<\/jats:italic>\n                    ,\n                    <jats:italic>Notch1<\/jats:italic>\n                    , and\n                    <jats:italic>Notch2<\/jats:italic>\n                    expressions (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), while increasing\n                    <jats:italic>Bmp2<\/jats:italic>\n                    . In contrast, at 60 PND,\n                    <jats:italic>Axin2<\/jats:italic>\n                    ,\n                    <jats:italic>Notch1<\/jats:italic>\n                    , and\n                    <jats:italic>Notch2<\/jats:italic>\n                    transcripts were elevated after EW (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Notch1 and Notch2 distribution indicated that EW might shift Notch2 to the upper gland, potentially affecting the fate of progenitor cells in pups.\n                    <jats:italic>In silico<\/jats:italic>\n                    analyses showed that zymogenic cells are in a renewal\u2010promoting environment, while parietal cells contribute to differentiation and signaling. Therefore, EW restricted the proliferative activity to the isthmus, altering the spatiotemporal expression of genes involved in stemness and differentiation, and reshaped the distribution of Notch receptors, without affecting the distribution of its downstream effector Hes1. These spatially organized molecular landscapes are crucial for proper epithelial renewal and function, and our data suggest that early nutritional disruption reprograms these niches with long\u2010term consequences.\n                  <\/jats:p>","DOI":"10.1002\/jcp.70234","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:58:57Z","timestamp":1790333937000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Early\u2010Life Nutritional Disruption Reshapes Gastric Epithelial Development Through Coordinated Changes in the Dynamics and Signaling of Proliferative Niches"],"prefix":"10.1002","volume":"241","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0830-8651","authenticated-orcid":false,"given":"Isadora Campos","family":"Rattes","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology, Institute of Biomedical Sciences University of Sao Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9836-9444","authenticated-orcid":false,"given":"Marcos Aur\u00e9lio Santos da","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Institute of Biomedical Sciences University of Sao Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5122-1602","authenticated-orcid":false,"given":"Viviane Rosa","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Institute of Biomedical Sciences University of Sao Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1863-893X","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Gama","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Institute of Biomedical Sciences University of Sao Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2024.04.016"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/dgd.12191"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142956.cy0738s55"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.09.037"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2113061118"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201490583"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00325.2016"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0185(20001101)260:3<213::AID-AR10>3.0.CO;2-9"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2013.09.003"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2015-310811"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2019.07.008"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-022-01079-4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092360202"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101737"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202305093"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.721242"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2009.09.001"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2011.00733.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066651"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02700"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.70021"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21010196"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2016.05.006"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202413019"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep45867"}],"container-title":["Journal 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