{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:27:45Z","timestamp":1773275265679,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T00:00:00Z","timestamp":1373846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000085","name":"Ontario HIV Treatment Network","doi-asserted-by":"crossref","award":["ROG 473"],"award-info":[{"award-number":["ROG 473"]}],"id":[{"id":"10.13039\/501100000085","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Clinical Pharma"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The use of antiretroviral therapy (ART) as pre\u2010exposure prophylaxis (PrEP) has gained global attention as a promising HIV prevention strategy in men who have sex with men. Permeability of these agents in the rectal mucosa may be partially regulated by interactions with drug efflux transporters, P\u2010glycoprotein (P\u2010gp), multidrug resistance\u2010associated proteins (MRPs) and\/or breast cancer resistance protein (BCRP). The objective of this work was to investigate the expression of drug efflux transporters in recto\u2010sigmoid colon tissues of HIV\u2010infected and uninfected men, and evaluate the association of ART and\/or HIV infection with drug transporter expression. MDR1\/P\u2010gp, MRPs (1\u20134) and BCRP mRNA and protein expression were detected in sigmoid colon biopsies of HIV\u2010uninfected individuals. Biopsies from HIV\u2010infected, ART\u2010na\u00efve participants revealed a significant downregulation of P\u2010gp and MRP2 protein levels compared to HIV\u2010uninfected individuals. Biopsies from HIV\u2010infected ART\u2010treated patients showed 1.9\u2010fold higher P\u2010gp protein expression and 1.5\u2010fold higher MRP2 protein expression compared to the ones obtained from the HIV\u2010infected ART\u2010na\u00efve patients. This is a first report demonstrating that HIV infection or ART could alter expression of drug efflux transporters in gut mucosa which in turn could affect the permeability of PrEP antiretroviral agents across this barrier, a highly vulnerable site of HIV transmission.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcph.132","type":"journal-article","created":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T13:58:46Z","timestamp":1373896726000},"page":"934-945","source":"Crossref","is-referenced-by-count":26,"title":["Expression of Membrane Drug Efflux Transporters in the Sigmoid Colon of HIV\u2010Infected and Uninfected Men"],"prefix":"10.1002","volume":"53","author":[{"given":"Mar\u00eda Fabiana","family":"De Rosa","sequence":"first","affiliation":[{"name":"Leslie Dan Faculty of Pharmacy University of Toronto Toronto Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin R.","family":"Robillard","sequence":"additional","affiliation":[{"name":"Leslie Dan Faculty of Pharmacy University of Toronto Toronto Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connie J.","family":"Kim","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Toronto Toronto Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. 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