{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:53:24Z","timestamp":1773017604264,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["accp1.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Clinical Pharma"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pregnancy induces physiological changes that can alter drug disposition, yet little is known about their impact on renal transporters such as organic anion transporters 1 and 3 (OAT1\/3). This study aimed to evaluate the in vivo activity of OAT1\/3 during pregnancy using furosemide as a probe substrate. Twelve healthy non\u2010pregnant women and 10 healthy pregnant women, mostly in the third trimester, received a single 40\u2010mg oral dose of furosemide under fasting conditions. Serial blood and urine samples were collected for up to 24 h. Pharmacokinetic parameters were estimated by non\u2010compartmental analysis, including time to maximum plasma concentration (t\n                    <jats:sub>max<\/jats:sub>\n                    ), maximum plasma concentration (C\n                    <jats:sub>max<\/jats:sub>\n                    ), area under the plasma concentration\u2013time curve (AUC), amount excreted in urine (Ae), fraction excreted unchanged in urine (f\n                    <jats:sub>e<\/jats:sub>\n                    ), unbound plasma fraction (\n                    <jats:italic>fu<\/jats:italic>\n                    ), apparent clearance (CL\/F), renal clearance (CL\n                    <jats:sub>R<\/jats:sub>\n                    ), non\u2010renal clearance (CL\n                    <jats:sub>NR<\/jats:sub>\n                    ), secretory clearance (CL\n                    <jats:sub>SEC<\/jats:sub>\n                    ), and metabolic clearance via furosemide glucuronide formation (CL\n                    <jats:sub>M<\/jats:sub>\n                    ). Compared with non\u2010pregnant women, pregnant women exhibited significantly lower exposure, C\n                    <jats:sub>max<\/jats:sub>\n                    , Ae, and f\n                    <jats:sub>e<\/jats:sub>\n                    values, while CL\/F and CL\n                    <jats:sub>NR<\/jats:sub>\n                    were significantly increased. In contrast, no significant differences were observed for CL\n                    <jats:sub>R<\/jats:sub>\n                    and CL\n                    <jats:sub>SEC,<\/jats:sub>\n                    indicating preserved OAT1\/3 activity. These findings suggest unchanged OAT1\/3\u2010mediated renal secretory activity during pregnancy contrast with the published literature for other OAT1\/3 substrates, which have, in most cases, reported an increase in OAT1\/3 activity during pregnancy. Instead, the data raise the hypothesis that changes in intestinal absorption of furosemide, possibly influenced by gestational regulation of intestinal transporters, may contribute to the lower exposure.\n                  <\/jats:p>","DOI":"10.1002\/jcph.70167","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:26:11Z","timestamp":1772994371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lack of Modulation of\n                    <i>In Vivo<\/i>\n                    Activity of Organic Anion Transporters 1 and 3 in Pregnancy Using Furosemide as a Probe"],"prefix":"10.1002","volume":"66","author":[{"given":"J\u00falia Werner","family":"Vieira","sequence":"first","affiliation":[{"name":"Department of Pharmacy School of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo 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