{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:46:28Z","timestamp":1790693188594,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,2,15]],"date-time":"2016-02-15T00:00:00Z","timestamp":1455494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Institut National de la Recherche Agronomique (INRA, France)"},{"DOI":"10.13039\/501100008965","name":"Soci\u00e9t\u00e9 Fran\u00e7aise de Nutrition","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008965","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Muscle wasting prevails in numerous diseases (e.g. diabetes, cardiovascular and kidney diseases, COPD,\u2026) and increases healthcare costs. A major clinical issue is to devise new strategies preventing muscle wasting. We hypothesized that 8\u2010week docosahexaenoic acid (DHA) supplementation prior to fasting may preserve muscle mass\n                      <jats:italic>in vivo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Six\u2010week\u2010old C57BL\/6 mice were fed a DHA\u2010enriched or a control diet for 8\u2009weeks and then fasted for 48\u2009h.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Feeding mice a DHA\u2010enriched diet prior to fasting elevated muscle glycogen contents, reduced muscle wasting, blocked the 55% decrease in Akt phosphorylation, and reduced by 30\u201340% the activation of AMPK, ubiquitination, or autophagy. The DHA\u2010enriched diet fully abolished the fasting induced\u2010messenger RNA (mRNA) over\u2010expression of the endocannabinoid receptor\u20101. Finally, DHA prevented or modulated the fasting\u2010dependent increase in muscle mRNA levels for Rab18, PLD1, and perilipins, which determine the formation and fate of lipid droplets, in parallel with muscle sparing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These data suggest that 8\u2010week DHA supplementation increased energy stores that can be efficiently mobilized, and thus preserved muscle mass in response to fasting through the regulation of Akt\u2010 and AMPK\u2010dependent signalling pathways for reducing proteolysis activation. Whether a nutritional strategy aiming at increasing energy status may shorten recovery periods in clinical settings remains to be tested.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12103","type":"journal-article","created":{"date-parts":[[2016,2,15]],"date-time":"2016-02-15T02:52:26Z","timestamp":1455504746000},"page":"587-603","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Docosahexaenoic acid\u2010supplementation prior to fasting prevents muscle atrophy in mice"],"prefix":"10.1002","volume":"7","author":[{"given":"Christiane","family":"Deval","sequence":"first","affiliation":[{"name":"INRA, UMR 1019 UNH, CRNH  F\u201063000 Auvergne Clermont\u2010Ferrand France"},{"name":"Clermont Universit\u00e9, Universit\u00e9 d'Auvergne Unit\u00e9 de Nutrition Humaine  BP 10448 F\u201063000 Clermont\u2010Ferrand 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