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The present study was therefore designed to investigate the effects of ageing, physical activity, and pre\u2010frailty on skeletal muscle phenotype, and mitochondrial and intramyocellular lipid content in men.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Recreationally active young adult (20\u201330\u2009yo; YA); active (ACT) and sedentary (SED) middle\u2010age (50\u201365\u2009yo; MA\u2010ACT and MA\u2010SED); and older (65\u2009+\u2009yo; 65\u2009+\u2009ACT and 65\u2009+\u2009SED) and pre\u2010frail older (65\u2009+\u2009PF) men were recruited. Muscle biopsies from the vastus lateralis were collected to assess, on muscle cross sections, muscle phenotype (using myosin heavy chain isoforms immunolabelling), the fibre type\u2010specific content of mitochondria (by quantifying the succinate dehydrogenase stain intensity), and the fibre type\u2010specific lipid content (by quantifying the Oil Red O stain intensity).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Only 65\u2009+\u2009SED and 65\u2009+\u2009PF displayed significantly lower overall and type IIa fibre sizes vs. YA. 65\u2009+\u2009SED displayed a lower type IIa fibre proportion vs. YA. MA\u2010SED and 65\u2009+\u2009SED displayed a higher hybrid type IIa\/IIx fibre proportion vs. YA. Sedentary and pre\u2010frail, but not active, men displayed lower mitochondrial content irrespective of fibre type vs. YA. 65\u2009+\u2009SED, but not 65\u2009+\u2009ACT, displayed a higher lipid content in type I fibres vs. YA. Finally, mitochondrial content, but not lipid content, was positively correlated with indices of muscle function, functional capacity, and insulin sensitivity across all subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Taken altogether, our results indicate that ageing in sedentary men is associated with (i) complex changes in muscle phenotype preferentially affecting type IIa fibres; (ii) a decline in mitochondrial content affecting all fibre types; and (iii) an increase in lipid content in type I fibres. They also indicate that physical activity partially protects from the effects of ageing on muscle phenotype, mitochondrial content, and lipid accumulation. No skeletal specific muscle phenotype of pre\u2010frailty was observed.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.12139","type":"journal-article","created":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T12:13:12Z","timestamp":1472818392000},"page":"213-228","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":146,"title":["The impact of ageing, physical activity, and pre\u2010frailty on skeletal muscle phenotype, mitochondrial content, and intramyocellular lipids in men"],"prefix":"10.1002","volume":"8","author":[{"given":"F\u00e9lix","family":"St\u2010Jean\u2010Pelletier","sequence":"first","affiliation":[{"name":"D\u00e9partement de Sciences de l'activit\u00e9 Physique, Facult\u00e9 des Sciences UQAM  Quebec Canada"},{"name":"Groupe de Recherche en Activit\u00e9 Physique Adapt\u00e9e  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte 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