{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T15:49:54Z","timestamp":1772034594301,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,10,10]],"date-time":"2016-10-10T00:00:00Z","timestamp":1476057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Pharma-Essentia, Taipei, Taiwan","award":["MOHW105-TDU-B-212-133019"],"award-info":[{"award-number":["MOHW105-TDU-B-212-133019"]}]},{"name":"Taiwan Ministry of Health and Welfare Clinical Trial and Research Center of Excellence"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Doxorubicin, a widely used anti\u2010tumour drug, is known to cause muscle loss in cancer patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Following an acute dose of doxorubicin injection (2.5\u2009mg\/kg per body weight), we examined macrophage distribution in rat soleus muscle challenged by eccentric exercise (downhill running). Long\u2010term doxorubicin treatment (one injection every 3\u2009days) on muscle mass and survival were also determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Under non\u2010exercised condition, increased tumour necrosis factor (TNF)\u2010alpha mRNA and decreased IL\u201010 mRNA were observed in soleus muscle of doxorubicin\u2010treated rats, compared with saline\u2010treated control rats. However, increases in inflammation score (leukocyte infiltration), nitrotyrosine level, and M1 macrophage (CD68<jats:sup>+<\/jats:sup>) invasion in exercised soleus muscle were absent in doxorubicin\u2010treated rats, whereas increased M2 macrophage (CD163<jats:sup>+<\/jats:sup>) localization in exercised muscle was less affected by doxorubicin. Despites coenzyme Q (Q10) supplementation significantly elevated TNF\u2010alpha mRNA, nitrotyrosine, and anti\u2010oxidant gamma\u2010glutamylcysteine synthetase (GCS) levels in non\u2010exercised soleus muscle, these pro\u2010inflammatory responses were also abolished in doxorubicin\u2010treated rats. Results from long\u2010term doxorubicin treatment show a significant muscle loss followed by an accelerated death, which cannot be reversed by Q10 supplementation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>(i) Doxorubicin impairs inflammation mechanism by depleting M1 macrophage in exercised skeletal muscle; (ii) Muscle loss and accelerated death during prolonged doxorubicin treatment cannot be reversed by Q10 supplementation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.12148","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T13:07:22Z","timestamp":1476364042000},"page":"277-284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Doxorubicin inhibits muscle inflammation after eccentric exercise"],"prefix":"10.1002","volume":"8","author":[{"given":"Sheng\u2010Chih","family":"Huang","sequence":"first","affiliation":[{"name":"Laboratory of Exercise Biochemistry University of Taipei  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin\u2010Fu","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Exercise Biochemistry University of Taipei  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suchada","family":"Saovieng","sequence":"additional","affiliation":[{"name":"Laboratory of Exercise Biochemistry University of Taipei  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Horng","family":"Chien","sequence":"additional","affiliation":[{"name":"Laboratory of Exercise Biochemistry University of Taipei  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Fen","family":"Hsu","sequence":"additional","affiliation":[{"name":"Laboratory of Exercise Biochemistry University of Taipei  Taipei 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