{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T13:17:19Z","timestamp":1792761439450,"version":"4.5.0"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T00:00:00Z","timestamp":1508112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Inadequate intake of micronutrients with antioxidant properties is common among older adults and has been associated with higher risk of frailty, adverse functional outcome, and impaired muscle health. However, a causal relationship is less well known. The aim was to determine in old mice the impact of reduced dietary intake of vitamins A\/E\/B6\/B12\/folate, selenium, and zinc on muscle mass, oxidative capacity, strength, and physical activity (PA) over time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010one\u2010month\u2010old male mice were fed either AIN\u201093\u2010M (control) or a diet low in micronutrients with antioxidant properties (=LOWOX\u2010B: 50% of mouse recommended daily intake of vitamins A, E, B6, and B12, folate, selenium, and zinc) for 4\u00a0months. Muscle mass, grip strength, physical activity (PA), and general oxidative status were assessed. Moreover, muscle fatigue was measured of m. <jats:italic>extensor digitorum longus<\/jats:italic> (EDL) during an <jats:italic>ex vivo<\/jats:italic> moderate exercise protocol. Effects on oxidative capacity [succinate dehydrogenase (SDH) activity], muscle fibre type, number, and fibre cross\u2010sectional area (fCSA) were assessed on m. <jats:italic>plantaris<\/jats:italic> (PL) using histochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 2\u00a0months on the diet, bodyweight of LOWOX\u2010B mice was lower compared with control (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001), mainly due to lower fat mass (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001), without significant differences in food intake. After 4\u00a0months, oxidative status of LOWOX\u2010B mice was lower, demonstrated by decreased vitamin E plasma levels (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and increased liver malondialdehyde levels (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.018). PA was lower in LOWOX\u2010B mice (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001 vs. control). Muscle mass was not affected, although PL\u2010fCSA was decreased (~16%; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.028 vs. control). SDH activity and muscle fibre type distribution remained unaffected. In LOWOX\u2010B mice, EDL force production was decreased by 49.7% at lower stimulation frequencies (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.038), and fatigue resistance was diminished (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.023) compared with control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Reduced dietary intake of vitamins A, E, B6, and B12, folate, selenium, and zinc resulted in a lower oxidative capacity and has major impact on muscle health as shown by decreased force production and PA, without effects on muscle mass. The reduced fCSA in combination with similar SDH activity per fibre might explain the reduced oxidative capacity resulting in the increased fatigue after exercise in LOWOX\u2010B mice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.12237","type":"journal-article","created":{"date-parts":[[2017,10,17]],"date-time":"2017-10-17T03:22:40Z","timestamp":1508210560000},"page":"146-159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Reduced dietary intake of micronutrients with antioxidant properties negatively impacts muscle health in aged mice"],"prefix":"10.1002","volume":"9","author":[{"given":"Miriam","family":"van Dijk","sequence":"first","affiliation":[{"name":"Nutricia Research, Nutricia Advanced Medical Nutrition  Uppsalalaan 12 3584 CT Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francina J.","family":"Dijk","sequence":"additional","affiliation":[{"name":"Nutricia 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