{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:59:23Z","timestamp":1790513963106,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004923","name":"AFM-T\u00e9l\u00e9thon","doi-asserted-by":"publisher","award":["19521"],"award-info":[{"award-number":["19521"]}],"id":[{"id":"10.13039\/501100004923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Muscle wasting is observed in the course of many diseases and also during physiological conditions (disuse, ageing). Skeletal muscle mass is largely controlled by the ubiquitin\u2010proteasome system and thus by the ubiquitinating enzymes (E2s and E3s) that target substrates for subsequent degradation. MuRF1 is the only E3 ubiquitin ligase known to target contractile proteins (\u03b1\u2010actin, myosins) during catabolic situations. However, MuRF1 depends on E2 ubiquitin\u2010conjugating enzymes for ubiquitin chain formation on the substrates. MuRF1\u2010E2 couples are therefore putative targets for preventing muscle wasting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We focused on 14 E2 enzymes that are either expressed in skeletal muscle or up\u2010regulated during atrophying conditions. In this work, we demonstrated that only highly sensitive and complementary interactomic approaches (surface plasmon resonance, yeast three\u2010hybrid, and split green fluorescent protein) allowed the identification of MuRF1 E2 partners.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five E2 enzymes physically interacted with MuRF1, namely, E2E1, E2G1, E2J1, E2J2, and E2L3. Moreover, we demonstrated that MuRF1\u2010E2E1 and MuRF1\u2010E2J1 interactions are facilitated by telethonin, a newly identified MuRF1 substrate. We next showed that the five identified E2s functionally interacted with MuRF1 since, in contrast to the non\u2010interacting E2D2, their co\u2010expression in HEK293T cells with MuRF1 led to increased telethonin degradation. Finally, we showed that telethonin governed the affinity between MuRF1 and E2E1 or E2J1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We report here the first MuRF1\u2010E2s network, which may prove valuable for deciphering the precise mechanisms involved in the atrophying muscle programme and for proposing new therapeutical approaches.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.12249","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T08:34:01Z","timestamp":1514104441000},"page":"129-145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["A muscle\u2010specific <scp>MuRF1\u2010E2<\/scp> network requires stabilization of <scp>MuRF1\u2010E2<\/scp> complexes by telethonin, a newly identified substrate"],"prefix":"10.1002","volume":"9","author":[{"given":"C\u00e9cile","family":"Polge","sequence":"first","affiliation":[{"name":"Universit\u00e9 Clermont 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N\u00e9phrologie\u2010Dialyse, Nutrition Clinique, Infectiologie R\u00e9animation M\u00e9dicale, Hygi\u00e8ne Hospitali\u00e8re (REUNNIRH)  F\u201063000 Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"B\u00e9chet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, INRA, UNH, Unit\u00e9 de Nutrition Humaine  CRNH Auvergne F\u201063000 Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydie","family":"Combaret","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, INRA, UNH, Unit\u00e9 de Nutrition Humaine  CRNH Auvergne F\u201063000 Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Attaix","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, INRA, UNH, Unit\u00e9 de Nutrition Humaine  CRNH Auvergne F\u201063000 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