{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T12:41:00Z","timestamp":1793882460172,"version":"4.7.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T00:00:00Z","timestamp":1520380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Skeletal muscle atrophy induced by either aging (sarcopenia) or mechanical unloading is associated with serious health consequences. Long non\u2010coding RNAs (lncRNAs) are implicated as important regulators in numerous physiological and pathological processes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Microarray analysis was performed to identify the differentially expressed lncRNAs in skeletal muscle between adult and aged mice. The most decreased lncRNA in aged skeletal muscle was identified. The C2C12 mouse myoblast cells were used to assess the biological function of the lncRNA\n                      <jats:italic>in vitro<\/jats:italic>\n                      . The target microRNA of lncRNA and the target protein of microRNA were predicted by bioinformatics analysis and validated\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Furthermore, the biology function of the lncRNA\n                      <jats:italic>in vivo<\/jats:italic>\n                      was investigated by local overexpression or knockdown the lncRNA in skeletal muscle. The therapeutic effect of the lncRNA overexpression in age\u2010related or mechanical unloading\u2010induced muscle atrophy was also evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We identified a novel lncRNA (muscle anabolic regulator 1, MAR1) which was highly expressed in mice skeletal muscle and positively correlated with muscle differentiation and growth\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      . We predicted and validated that microRNA\u2010487b (miR\u2010487b) was a direct target of MAR1. We also predicted and validated that Wnt5a, an important regulator during myogenesis, was a target of miR\u2010487b in C2C12 cells. Our findings further demonstrated that enforced MAR1 expression in myoblasts led to derepression of Wnt5a. Moreover, MAR1 promoted skeletal muscle mass\/strength and Wnt5a protein level in mice. Enforced MAR1 expression in mice attenuated muscle atrophy induced by either aging or unloading.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The newly identified lncRNA MAR1 acts as a miR\u2010487b sponge to regulate Wnt5a protein, resulting in promoting muscle differentiation and regeneration. MAR1 could be a novel therapeutic target for treating muscle atrophy induced by either aging or mechanical unloading.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12281","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T01:39:35Z","timestamp":1520386775000},"page":"613-626","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":165,"title":["A newly identified lncRNA MAR1 acts as a miR\u2010487b sponge to promote skeletal muscle differentiation and regeneration"],"prefix":"10.1002","volume":"9","author":[{"given":"Zong\u2010Kang","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Chinese Medicine, Faculty of Medicine The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine, Faculty of Medicine The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daogang","family":"Guan","sequence":"additional","affiliation":[{"name":"Institute of Integrated Bioinformedicine and Translational Science, School of Chinese Medicine Hong Kong Baptist University  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Liang","sequence":"additional","affiliation":[{"name":"Law Sau Fai Institute for Advancing Translational Medicine in Bone &amp; Joint Diseases, School of Chinese Medicine Hong Kong Baptist University  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenjian","family":"Zhuo","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine, Faculty of Medicine The Chinese University of Hong Kong  Hong Kong SAR 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