{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:55:02Z","timestamp":1789779302360,"version":"4.0.1"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T00:00:00Z","timestamp":1520294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","award":["NTUH105\u2010S2953"],"award-info":[{"award-number":["NTUH105\u2010S2953"]}],"id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST105\u20102314\u2010B\u2010002\u2010025\u2010MY3"],"award-info":[{"award-number":["MOST105\u20102314\u2010B\u2010002\u2010025\u2010MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      A global consensus on the loss of skeletal muscle mass and function in humans refers as sarcopenia and cachexia including diabetes, obesity, renal failure, and osteoporosis. Despite a current improvement of sarcopenia or cachexia with exercise training and supportive therapies, alternative and specific managements are needed to discover for whom are unable or unwilling to embark on these treatments. Alendronate is a widely used drug for osteoporosis in the elderly and postmenopausal women. Osteopenic menopausal women with 6\u00a0months of alendronate therapy have been observed to improve not only lumbar bone mineral density but also handgrip strength. However, the effect and mechanism of alendronate on muscle strength still remain unclear. Here, we investigated the therapeutic potential and the molecular mechanism of alendronate on the loss of muscle mass and strength\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mouse myoblasts and primary human skeletal muscle\u2010derived progenitor cells were used to assess the effects of low\u2010dose alendronate (0.1\u20131\u00a0\u03bcM) combined with or without dexamethasone on myotube hypertrophy and myogenic differentiation. Moreover, we also evaluated the effects of low\u2010dose alendronate (0.5 and 1\u00a0mg\/kg) by oral administration on the limb muscle function and morphology of mice with denervation\u2010induced muscle atrophy and glycerol\u2010induced muscle injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Alendronate inhibited dexamethasone\u2010induced myotube atrophy and myogenic differentiation inhibition in mouse myoblasts and primary human skeletal muscle\u2010derived progenitor cells. Alendronate significantly abrogated dexamethasone\u2010up\u2010regulated sirtuin\u20103 (SIRT3), but not SIRT1, protein expression in myotubes. Both SIRT3 inhibitor AKG7 and SIRT3\u2010siRNA transfection could also reverse dexamethasone\u2010up\u2010regulated atrogin\u20101 and SIRT3 protein expressions. Animal studies showed that low\u2010dose alendronate by oral administration ameliorated the muscular malfunction in mouse models of denervation\u2010induced muscle atrophy and glycerol\u2010induced muscle injury with a negative regulation of SIRT3 expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The putative mechanism by which muscle atrophy was improved with alendronate might be through the SIRT3 down\u2010regulation. These findings suggest that alendronate can be a promising therapeutic strategy for management of muscle wasting\u2010related diseases and sarcopenia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12289","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T01:19:55Z","timestamp":1520385595000},"page":"585-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Preventing muscle wasting by osteoporosis drug alendronate\n                    <i>in vitro<\/i>\n                    and in myopathy models via sirtuin\u20103 down\u2010regulation"],"prefix":"10.1002","volume":"9","author":[{"given":"Hsien\u2010Chun","family":"Chiu","sequence":"first","affiliation":[{"name":"Institute of Toxicology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen\u2010Yuan","family":"Chiu","sequence":"additional","affiliation":[{"name":"Institute of Toxicology, College of Medicine National Taiwan University  Taipei Taiwan"},{"name":"Institute of Food Safety and Health College of Public Health, National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong\u2010Sen","family":"Yang","sequence":"additional","affiliation":[{"name":"Departments of Orthopaedics, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding\u2010Cheng","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Geriatrics and Gerontology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shing\u2010Hwa","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Toxicology, College of Medicine National Taiwan University  Taipei Taiwan"},{"name":"Department of Medical Research, China Medical University Hospital China Medical University  Taichung Taiwan"},{"name":"Department of Pediatrics, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih\u2010Kang","family":"Chiang","sequence":"additional","affiliation":[{"name":"Institute of Toxicology, College of Medicine National Taiwan University  Taipei Taiwan"},{"name":"Department of Internal Medicine, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/61.10.1059"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2014.161"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2000.109103"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4467"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.0901525"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfu003"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glt149"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2001.281.2.E197"},{"key":"e_1_2_8_10_1","first-page":"156","article-title":"Sarcopenia and muscle function during menopause and hormone\u2010replacement therapy","volume":"4","author":"Dionne IJ","year":"2000","journal-title":"J Nutr Health Aging"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1017535728844"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)08827-X"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.6.785"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.111.5.e601"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0b013e31827cabca"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.afos.2015.07.005"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.gme.0000227861.35226.fa"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12097"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12050"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12202"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12122"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12054"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12063"},{"key":"e_1_2_8_24_1","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"Institute of Laboratory Animal Resources","year":"2011"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.014126"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4674"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90679-C"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.34.147"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200202092"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00114.2011"},{"key":"e_1_2_8_31_1","first-page":"611","article-title":"Acylated and unacylated ghrelin impair skeletal muscle atrophy in mice","volume":"123","author":"Porporato PE","year":"2013","journal-title":"J Clin Invest"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-14-0490"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065874"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00022.2011"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00019.2003"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e3182667c2e"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2006.09.009"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2015.09.015"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2162"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2015.02.018"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12171"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000342502"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1570355"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-015-0993-7"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111334109"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/09168451.2016.1210502"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8619(03)00053-7"},{"key":"e_1_2_8_48_1","unstructured":"MillerML.Glucocorticoid\u2010induced myopathy. version 16.3. 2009."},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008483-199703000-00002"},{"key":"e_1_2_8_50_1","first-page":"12","article-title":"Sarcopenic obesity and endocrinal adaptation with age","volume":"1","author":"Sakuma K","year":"2013","journal-title":"Int J Endocrinol"},{"key":"e_1_2_8_51_1","article-title":"European Renal Nutrition (ERN) and Chronic Kidney Disease\u2010Mineral and Bone Disorder (CKD\u2010MBD) Working Groups of the European Renal Association\u2010European Dialysis Transplant Association (ERA\u2010EDTA). Vitamin D, a modulator of musculoskeletal health in chronic kidney disease","author":"Molina P","year":"2017","journal-title":"J Cachexia Sarcopenia Muscle"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/35001622"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3000"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0897"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e05-01-0033"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2007-0079"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543776.2014.982532"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00226-0"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.489716"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.2337\/db12-1650"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085636"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00504.2006"},{"key":"e_1_2_8_63_1","article-title":"Downregulation of angiotensin type 1 receptor and nuclear factor\u2010\u03baB by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction","volume":"6","author":"Yang SY","year":"2016","journal-title":"Sci Rep"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24748"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00586-15"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12089"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcsm.12289","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12289","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T12:06:43Z","timestamp":1695470803000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.12289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,6]]},"references-count":65,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1002\/jcsm.12289"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.12289","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.732830601.793543691","asserted-by":"object"}]},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,6]]},"assertion":[{"value":"2017-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}