{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:59:34Z","timestamp":1792115974233,"version":"4.4.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,4]],"date-time":"2018-10-04T00:00:00Z","timestamp":1538611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["282310\u2010MyoPHAGY"],"award-info":[{"award-number":["282310\u2010MyoPHAGY"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Perilipin2 (Plin2) belongs to a family of five highly conserved proteins, known for their role in lipid storage. Recent data indicate that Plin2 has an important function in cell metabolism and is involved in several human pathologies, including liver steatosis and Type II diabetes. An association between Plin2 and lower muscle mass and strength has been found in elderly and inactive people, but its function in skeletal muscle is still unclear. Here, we addressed the role of Plin2 in adult muscle by gain and loss of function experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      By mean of\n                      <jats:italic>in vivo<\/jats:italic>\n                      Plin2 down\u2010regulation (shPlin2) and overexpression (overPlin2) in murine\n                      <jats:italic>tibialis anterior<\/jats:italic>\n                      muscle, we analysed the effects of Plin2 genetic manipulations on myofiber size and lipid composition. An analysis of skeletal muscle lipid composition was also performed in\n                      <jats:italic>vastus lateralis<\/jats:italic>\n                      samples from young and old patients undergoing hip surgery.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We found that Plin2 down\u2010regulation was sufficient to induce a 30% increase of myofiber cross\u2010sectional area, independently of mTOR pathway. Alterations of lipid content and modulation of genes involved in lipid synthesis occurred in hypertrophic muscles. In particular, we showed a decrease of triglycerides, ceramides, and phosphatidylcoline:phosphatidylethanolamine ratio, a condition known to impact negatively on muscle function. Plin2 overexpression did not change fibre size; however, lipid composition was strongly affected in a way that is similar to that observed in human samples from old patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Altogether these data indicate that Plin2 is a critical mediator for the control of muscle mass, likely, but maybe not exclusively, through its critical role in the regulation of intracellular lipid content and composition.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12355","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T23:54:00Z","timestamp":1538783640000},"page":"95-110","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Muscle\u2010specific Perilipin2 down\u2010regulation affects lipid metabolism and induces myofiber hypertrophy"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4621-9898","authenticated-orcid":false,"given":"Maria","family":"Conte","sequence":"first","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine (DIMES) University of Bologna  Bologna Italy"},{"name":"Interdepartmental Centre \u201cL. Galvani\u201d (CIG) University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Armani","sequence":"additional","affiliation":[{"name":"Venetian Institute of Molecular Medicine (VIMM)  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Conte","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and Environment University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Serra","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and Environment University of Pisa  Pisa Italy"},{"name":"Research Center of Nutraceuticals and Food for Health University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Franceschi","sequence":"additional","affiliation":[{"name":"IRCCS Institute of Neurological Sciences of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Mele","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and Environment University of Pisa  Pisa Italy"},{"name":"Research Center of Nutraceuticals and Food for Health University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Sandri","sequence":"additional","affiliation":[{"name":"Venetian Institute of Molecular Medicine (VIMM)  Padova Italy"},{"name":"Department of Biomedical Science University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Salvioli","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine (DIMES) University of Bologna  Bologna Italy"},{"name":"Interdepartmental Centre \u201cL. 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