{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:18:36Z","timestamp":1791591516516,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,30]],"date-time":"2019-01-30T00:00:00Z","timestamp":1548806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS\u201041\u2010G03"],"award-info":[{"award-number":["CARS\u201041\u2010G03"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2018A030310209"],"award-info":[{"award-number":["2018A030310209"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Recent studies indicate important roles for long noncoding RNAs (lncRNAs) in the regulation of gene expression by acting as competing endogenous RNAs (ceRNAs). However, the specific role of lncRNAs in skeletal muscle atrophy is still unclear. Our study aimed to identify the function of lncRNAs that control skeletal muscle myogenesis and atrophy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      RNA sequencing was performed to identify the skeletal muscle transcriptome (lncRNA and messenger RNA) between hypertrophic broilers and leaner broilers. To study the \u2018sponge\u2019 function of lncRNA, we constructed a lncRNA\u2010microRNA (miRNA)\u2010gene interaction network by integrated our previous submitted skeletal muscle miRNA sequencing data. The primary myoblast cells and animal model were used to assess the biological function of the\n                      <jats:italic>lncIRS1 in vitro<\/jats:italic>\n                      or\n                      <jats:italic>in vivo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We constructed a myogenesis\u2010associated lncRNA\u2010miRNA\u2010gene network and identified a novel ceRNA lncRNA named\n                      <jats:italic>lncIRS1<\/jats:italic>\n                      that is specifically enriched in skeletal muscle.\n                      <jats:italic>LncIRS1<\/jats:italic>\n                      could regulate myoblast proliferation and differentiation\n                      <jats:italic>in vitro<\/jats:italic>\n                      , and muscle mass and mean muscle fibre\n                      <jats:italic>in vivo<\/jats:italic>\n                      .\n                      <jats:italic>LncIRS1<\/jats:italic>\n                      increases gradually during myogenic differentiation. Mechanistically,\n                      <jats:italic>lncIRS1<\/jats:italic>\n                      acts as a ceRNA for miR\u201015a, miR\u201015b\u20105p, and miR\u201015c\u20105p to regulate\n                      <jats:italic>IRS1<\/jats:italic>\n                      expression, which is the downstream of the\n                      <jats:italic>IGF1<\/jats:italic>\n                      receptor. Overexpression of\n                      <jats:italic>lncIRS1<\/jats:italic>\n                      not only increased the protein abundance of\n                      <jats:italic>IRS1<\/jats:italic>\n                      but also promoted phosphorylation level of AKT (p\u2010AKT) a central component of insulin\u2010like growth factor\u20101 pathway. Furthermore,\n                      <jats:italic>lncIRS1<\/jats:italic>\n                      regulates the expression of atrophy\u2010related genes and can rescue muscle atrophy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The newly identified\n                      <jats:italic>lncIRS1<\/jats:italic>\n                      acts as a sponge for miR\u201015 family to regulate\n                      <jats:italic>IRS1<\/jats:italic>\n                      expression, resulting in promoting skeletal muscle myogenesis and controlling atrophy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12374","type":"journal-article","created":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T01:50:17Z","timestamp":1548899417000},"page":"391-410","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":173,"title":["<i>LncIRS1<\/i>\n                    controls muscle atrophy via sponging miR\u201015 family to activate IGF1\u2010PI3K\/AKT pathway"],"prefix":"10.1002","volume":"10","author":[{"given":"Zhenhui","family":"Li","sequence":"first","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong China"},{"name":"Guangdong Provincial Key Lab of Agro\u2010Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction Ministry of Agriculture  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bolin","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong China"},{"name":"Guangdong Provincial Key Lab of Agro\u2010Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction Ministry of Agriculture  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahareldin Ali","family":"Abdalla","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong China"},{"name":"Guangdong Provincial Key Lab of Agro\u2010Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction Ministry of Agriculture  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuenong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong China"},{"name":"Guangdong Provincial Key Lab of Agro\u2010Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction Ministry of Agriculture  Guangzhou Guangdong China"},{"name":"Institute of Biotechnology Nanchang Normal University  Nanchang 330032 Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong China"},{"name":"Guangdong Provincial Key Lab of Agro\u2010Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction Ministry of Agriculture  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peigong","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong China"},{"name":"Guangdong Provincial Key Lab of Agro\u2010Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction Ministry of Agriculture  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong China"},{"name":"Guangdong Provincial Key Lab of Agro\u2010Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction Ministry of Agriculture  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiquan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong China"},{"name":"Guangdong Provincial Key Lab of Agro\u2010Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction Ministry of Agriculture  Guangzhou Guangdong 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