{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T01:49:55Z","timestamp":1784771395743,"version":"3.55.0"},"reference-count":74,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,6,14]],"date-time":"2019-06-14T00:00:00Z","timestamp":1560470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20 GM109098"],"award-info":[{"award-number":["P20 GM109098"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20 RR016477"],"award-info":[{"award-number":["P20 RR016477"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 RR032138\/GM103488"],"award-info":[{"award-number":["P30 RR032138\/GM103488"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20 RR016440"],"award-info":[{"award-number":["P20 RR016440"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007271","name":"Health Science Center, University of Tennessee","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007271","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sirtuin 1 (SIRT1) is a NAD+ sensitive deacetylase that has been linked to longevity and has been suggested to confer beneficial effects that counter aging\u2010associated deterioration. Muscle repair is dependent upon satellite cell function, which is reported to be reduced with aging; however, it is not known if this is linked to an aging\u2010suppression of SIRT1. This study tested the hypothesis that Sirtuin 1 (SIRT1) overexpression would increase the extent of muscle repair and muscle function in older mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined satellite cell dependent repair in tibialis anterior, gastrocnemius, and soleus muscles of 13 young wild\u2010type mice (20\u201330\u00a0weeks) and 49 older (80+ weeks) mice that were controls (<jats:italic>n<\/jats:italic>\u00a0=\u00a013), overexpressed SIRT1 in skeletal muscle (<jats:italic>n<\/jats:italic>\u00a0=\u00a014), and had a skeletal muscle SIRT1 knockout (<jats:italic>n<\/jats:italic>\u00a0=\u00a012) or a satellite cell SIRT1 knockout (<jats:italic>n<\/jats:italic>\u00a0=\u00a010). Acute muscle injury was induced by injection of cardiotoxin (CTX), and phosphate\u2010buffered saline was used as a vector control. Plantarflexor muscle force and fatigue were evaluated before or 21\u00a0days after CTX injection. Satellite cell proliferation and mitochondrial function were also evaluated in undamaged muscles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Maximal muscle force was significantly lower in control muscles of older satellite cell knockout SIRT1 mice compared to young adult wild\u2010type (YWT) mice (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Mean contraction force at 40\u00a0Hz stimulation was significantly greater after recovery from CTX injury in older mice that overexpressed muscle SIRT1 than age\u2010matched SIRT1 knockout mice (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). SIRT1 muscle knockout models (P\u00a0&lt;\u00a00.05) had greater levels of p53 (P\u00a0&lt;\u00a00.05 MKO,<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001 OE) in CTX\u2010damaged tissues as compared to YWT CTX mice. SIRT1 overexpression with co\u2010expression of p53 was associated with increased fatigue resistance and increased force potentiation during repeated contractions as compared to wild\u2010type or SIRT1 knockout models (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Muscle structure and mitochondrial function were not different between the groups, but proliferation of satellite cells was significantly greater in older mice with SIRT1 muscle knockout (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), but not older SIRT1 satellite cell knockout models,<jats:italic>in vitro<\/jats:italic>, although this effect was attenuated<jats:italic>in vivo<\/jats:italic>after 21\u00a0days of recovery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The data suggest skeletal muscle structure, function, and recovery after CTX\u2010induced injury are not significantly influenced by gain or loss of SIRT1 abundance alone in skeletal muscle; however, muscle function is impaired by ablation of SIRT1 in satellite cells. SIRT1 appears to interact with p53 to improve muscle fatigue resistance after repair from muscle injury.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.12437","type":"journal-article","created":{"date-parts":[[2019,6,14]],"date-time":"2019-06-14T08:45:42Z","timestamp":1560501942000},"page":"929-949","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["The role of SIRT1 in skeletal muscle function and repair of older mice"],"prefix":"10.1002","volume":"10","author":[{"given":"Matthew J.","family":"Myers","sequence":"first","affiliation":[{"name":"Laboratory of Muscle Biology and Sarcopenia West Virginia University School of Medicine Morgantown USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle L.","family":"Shepherd","sequence":"additional","affiliation":[{"name":"Division of Exercise Physiology and Center for Cardiovascular and Respiratory Sciences West Virginia University School of Medicine Morgantown USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrya J.","family":"Durr","sequence":"additional","affiliation":[{"name":"Division of Exercise Physiology and Center for Cardiovascular and Respiratory Sciences West Virginia University School of Medicine Morgantown USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Stanton","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Biology and Sarcopenia West Virginia University School of Medicine Morgantown USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junaith S.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Biology and Sarcopenia West Virginia University School of Medicine Morgantown USA"},{"name":"Laboratory of Nerve and Muscle, Department of Clinical Laboratory Sciences, College of Health Professions University of Tennessee Health Science Center Memphis USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Hollander","sequence":"additional","affiliation":[{"name":"Division of Exercise Physiology and Center for Cardiovascular and Respiratory Sciences West Virginia University School of Medicine Morgantown USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen E.","family":"Alway","sequence":"additional","affiliation":[{"name":"Laboratory of Muscle Biology and Sarcopenia West Virginia University School of Medicine Morgantown USA"},{"name":"Laboratory of Muscle Biology and Sarcopenia, Department of Physical Therapy, College of Health Professions University of Tennessee Health Science Center Memphis USA"},{"name":"Department of Physiology, College of Medicine University of Tennessee Health Science Center Memphis USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/50A.Special_Issue.5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afu115"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0b013e328312c37d"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1249\/JES.0000000000000101"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01102.2001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2017.08.004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00298.2016"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2013.06.024"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100639"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1988.64.3.1114"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.012559"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2011.00695.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathol.4.110807.092250"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3293"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.489716"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121959"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-017-3236-1"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2009.02.006"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1016089107"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07813"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501485200"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.261685"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.4.3.1518"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00451.2017"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2017.08.008"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.01.006"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glx089"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2011.061028"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.12.004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013307"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.5115\/acb.2015.48.1.54"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glr047"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101358"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.16267"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035273"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.06.001"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.110"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100696"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.12.8719"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00239.2004"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2017.08.008"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2017.02.075"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00845.2013"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00467.2008"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00353.2015"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.04.002"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1515-4"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2008.02.017"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2013.02.012"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004490"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.11.034"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2016.02.002"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/62.3.235"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00668.2009"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21218"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2009.03.008"},{"key":"e_1_2_9_58_1","first-page":"640","article-title":"Age\u2010related differences in dystrophin: impact on force transfer proteins, membrane integrity, and neuromuscular junction stability","volume":"72","author":"Hughes DC","year":"2017","journal-title":"J Gerontol A Biol Sci Med Sci"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1089\/rej.2017.1989"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.022749"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00936.2012"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.01.028"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imr.2016.07.003"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019194"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.363747"},{"key":"e_1_2_9_66_1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.14348\/molcells.2017.0081","article-title":"Modulation of mitochondrial membrane potential and ROS generation by nicotinamide in a manner independent of SIRT1 and mitophagy","volume":"40","author":"Song SB","year":"2017","journal-title":"Mol Cells"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a026070"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40279-013-0127-9"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.212977"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00374.2005"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.151.6.1295"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00417776"},{"key":"e_1_2_9_73_1","first-page":"234","article-title":"Postactivation potentiation: an introduction","volume":"6","author":"Lorenz D","year":"2011","journal-title":"Int J Sports Phys Ther"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00495.2004"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12261"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcsm.12437","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12437","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.12437","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12437","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T18:58:35Z","timestamp":1721415515000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.12437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,14]]},"references-count":74,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1002\/jcsm.12437"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.12437","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,14]]},"assertion":[{"value":"2018-09-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}