{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T04:39:59Z","timestamp":1784867999841,"version":"3.55.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T00:00:00Z","timestamp":1580169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472097"],"award-info":[{"award-number":["81472097"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012479","name":"General Research Fund of Shanghai Normal University","doi-asserted-by":"publisher","award":["14103314"],"award-info":[{"award-number":["14103314"]}],"id":[{"id":"10.13039\/501100012479","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sarcopenia is an aging\u2010induced deterioration of skeletal muscle mass and function. Low\u2010magnitude high\u2010frequency vibration (LMHFV) was shown to improve muscle functions and \u03b2\u2010hydroxy\u2010\u03b2\u2010methylbutyrate (HMB) to increase muscle mass and strength. Muscle\u2010derived stem cells (MDSCs) are progenitor cells important for muscle regeneration. We hypothesized that LMHFV and HMB could retard sarcopenia by reducing fat infiltration through inhibiting adipogenesis in MDSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Senescence\u2010accelerated mouse P8 male mice were randomized into control (CTL), HMB, LMHFV (VIB), and combined (COM) groups. Interventions started at age of month 7 and assessed at 1, 2, and 3 months post\u2010intervention by densitometry, histology, and functional tests. <jats:italic>In vitro<\/jats:italic>, MDSCs isolated from gastrocnemius of senescence\u2010accelerated mouse P8 mice were characterized, randomized into CTL, VIB, HMB, and COM groups, and assessed by oil red O staining, mRNA, and protein expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At 2 months post\u2010intervention, percentage lean mass of HMB, VIB, and COM groups were significantly higher than CTL group. Twitch, tetanic, and specific tetanic forces of COM group were higher, while specific twitch force of both VIB and COM groups were higher. Grip strength of HMB, VIB, and COM groups were higher. Histologically, both VIB and COM groups presented lower oil red O area than CTL group. Type I muscle fibre in CTL group was higher than HMB, VIB, and COM groups. MDSC were detected <jats:italic>in situ<\/jats:italic> by immunofluorescence stain with stem cell antigen\u20101 signals confirmed with higher \u03b2\u2010catenin expression in the COM group. The observations were also confirmed <jats:italic>in vitro<\/jats:italic>, MDSCs in the HMB, VIB, and COM groups presented lower adipogenesis vs. the CTL group. \u03b2\u2010Catenin mRNA and protein expressions were lower in the CTL group while their relationship was further validated through \u03b2\u2010catenin knock\u2010down approach.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results showed that combined LMHFV and HMB interventions enhanced muscle strength and decreased percentage fat mass and intramuscular fat infiltration as compared with either treatment alone. Additive effect of LMHFV and HMB was demonstrated in \u03b2\u2010catenin expression than either treatment in MDSCs and altered cell fate from adipogenesis to myogenesis, leading to inhibition of intramuscular lipid accumulation. Wnt\/\u03b2\u2010catenin signalling pathway was found to be the predominant regulatory mechanism through which LMHFV and HMB combined treatment suppressed MDSCs adipogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.12535","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T01:16:15Z","timestamp":1580260575000},"page":"564-577","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Vibration and \u03b2\u2010hydroxy\u2010\u03b2\u2010methylbutyrate treatment suppresses intramuscular fat infiltration and adipogenic differentiation in sarcopenic mice"],"prefix":"10.1002","volume":"11","author":[{"given":"Jinyu","family":"Wang","sequence":"first","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong  Hong Kong SAR The People's Republic of 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Yeung","family":"Wong","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong  Hong Kong SAR The People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwok\u2010Sui","family":"Leung","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong  Hong Kong SAR The People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon Kwoon\u2010Ho","family":"Chow","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong  Hong Kong SAR The People's Republic of China"},{"name":"The CUHK-ACC Space Medicine Centre on Health Maintenance of Musculoskeletal System The Chinese University of Hong Kong Shenzhen 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