{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:25:28Z","timestamp":1790749528212,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T00:00:00Z","timestamp":1606348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003134","name":"Fonds pour la Formation \u00e0 la Recherche dans l\u2019Industrie et dans l\u2019Agriculture","doi-asserted-by":"publisher","award":["31618719"],"award-info":[{"award-number":["31618719"]}],"id":[{"id":"10.13039\/501100003134","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Non\u2010alcoholic fatty liver (NAFL) disease (NAFLD) is the most common chronic liver disease in the world. While most subjects have \u2018inert\u2019 NAFL, a subset will progress to non\u2010alcoholic steatohepatitis (NASH) and its life\u2010threatening complications. A substantial body of literature supports that a low muscle mass, low strength, and\/or muscle fatty infiltration (myosteatosis) are associated with NAFLD severity. Here, we evaluated the muscle compartment in NASH preclinical models to decipher the kinetics of muscle alterations in relation with liver disease progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We developed and validated a micro\u2010computed tomography\u2010based methodology to prospectively study skeletal muscle mass and density in muscle and liver (i.e. reflecting fatty infiltration) in a high\u2010throughput and non\u2010invasive manner in three preclinical NAFLD\/NASH rodent models: fat aussie (FOZ) mice fed a high\u2010fat diet (FOZ HF), wild\u2010type (WT) mice fed a high\u2010fat high\u2010fructose diet (WT HFF), and WT mice fed a high\u2010fat diet (WT HF). We compared them with WT mice fed a normal diet (WT ND) used as controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      \u2010FOZ HF with fibrosing NASH had sarcopenia characterized by a reduced muscle strength when compared with WT HF and WT HFF with early NASH and WT ND controls (165.2\u00a0\u00b1\u00a05.2\u00a0g vs. 237.4\u00a0\u00b1\u00a011.7\u00a0g, 256\u00a0\u00b1\u00a05.7\u00a0g, and 242.9\u00a0\u00b1\u00a09.3\u00a0g, respectively,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a060;\u00a00.001). Muscle mass or strength was not lower in FOZ HF, WT HF, and WT HFF with early NASH than in controls. Myosteatosis was present in FOZ HF with fibrosing NASH, but also in FOZ HF, WT HF, and WT HFF with early NASH (muscle density\u00a0=\u00a00.50\u00a0\u00b1\u00a00.02, 0.62\u00a0\u00b1\u00a00.02, 0.70\u00a0\u00b1\u00a00.05, and 0.75\u00a0\u00b1\u00a00.03, respectively, with\n                      <jats:italic>P<\/jats:italic>\n                      \u00a060;\u00a00.001 when compared with respective controls). Myosteatosis degree was strongly correlated with NAFLD activity score (\n                      <jats:italic>r<\/jats:italic>\n                      \u00a0=\u00a0\u22120.87,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a067,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a060;\u00a00.001). In multivariate analysis, the association between myosteatosis and NASH was independent from homeostatic model assessment of insulin resistance and visceral fat area (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a060;\u00a00.05). Myosteatosis degree powerfully discriminated NASH from benign NAFL and normal liver (area under the receiver operating characteristic\u00a0=\u00a00.96,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a067,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a060;\u00a00.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Taken together, our data support that there is no sarcopenia in obese mice with early NASH. In contrast, the severity of myosteatosis reflects on hepatocellular damage and inflammation during early NASH development. This observation prompts us to exploit myosteatosis as a novel non\u2010invasive marker of NASH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12646","type":"journal-article","created":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T23:14:21Z","timestamp":1606432461000},"page":"144-158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Myosteatosis rather than sarcopenia associates with non\u2010alcoholic steatohepatitis in non\u2010alcoholic fatty liver disease preclinical models"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2333-9213","authenticated-orcid":false,"given":"Maxime","family":"Nachit","sequence":"first","affiliation":[{"name":"Laboratory of Hepato\u2010Gastroenterology, Institute of Experimental and Clinical Research UCLouvain  Brussels Belgium"},{"name":"Department of Imaging and Pathology KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"De Rudder","sequence":"additional","affiliation":[{"name":"Laboratory of Hepato\u2010Gastroenterology, Institute of Experimental and Clinical Research UCLouvain  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Paul","family":"Thissen","sequence":"additional","affiliation":[{"name":"Pole of Endocrinology, Diabetes and Nutrition, Institute of Experimental and Clinical Research UCLouvain  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Schakman","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience UCLouvain  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Bouzin","sequence":"additional","affiliation":[{"name":"IREC Imaging Platform UCLouvain  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Horsmans","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9pato\u2010Gastro\u2010Ent\u00e9rologie Cliniques Universitaires Saint\u2010Luc  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greetje","family":"Vande Velde","sequence":"additional","affiliation":[{"name":"Department of Imaging and Pathology KU Leuven  Leuven Belgium"},{"name":"Molecular Small Animal Imaging Center (MoSAIC) KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle Anne","family":"Leclercq","sequence":"additional","affiliation":[{"name":"Laboratory of Hepato\u2010Gastroenterology, Institute of Experimental and Clinical Research UCLouvain  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.10.033"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29367"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0144-8"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29721"},{"key":"e_1_2_9_6_1","first-page":"1","article-title":"Implications of low muscle mass across the continuum of care: a narrative review","author":"Prado CM","year":"2018","journal-title":"Ann Med"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afy169"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)31138-9"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12039"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2019.05.034"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.25346"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29420"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ctg.2015.31"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30828"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.08.019"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.13889"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2019.04.023"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.12227"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26716"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20180421"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26908"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.14253"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12598"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-56121-7"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/obr.12776"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30333"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.02.032"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-019-0315-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-017-4340-1"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00016.2010"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20701"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25889"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bonr.2016.04.002"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1139\/H08-075"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2015.113"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037026"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0000000000001397"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28376"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2017.11.021"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep4.1435"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2018.12.036"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-020-1165-z"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00330-019-06573-2"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1721"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-019-4927-9"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.09.001"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.13513"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchf.2018.06.002"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.117.309633"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.21328"},{"key":"e_1_2_9_52_1","first-page":"72","article-title":"Psoas and paraspinous muscle measurements on computed tomography predict mortality in European Americans with type 2 diabetes mellitus","volume":"8","author":"Tucker BM","year":"2019","journal-title":"J Frailty Aging"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12501"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12646","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.12646","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12646","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T05:25:34Z","timestamp":1693632334000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.12646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,26]]},"references-count":52,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/jcsm.12646"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.12646","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,26]]},"assertion":[{"value":"2020-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}