{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:25:39Z","timestamp":1787235939388,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T00:00:00Z","timestamp":1616716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006477","name":"National Taiwan University","doi-asserted-by":"publisher","award":["10R891903"],"award-info":[{"award-number":["10R891903"]}],"id":[{"id":"10.13039\/501100006477","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["NHRI\u2010105\u20101053ISI"],"award-info":[{"award-number":["NHRI\u2010105\u20101053ISI"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 108\u20102320\u2010B\u2010002\u2010040"],"award-info":[{"award-number":["MOST 108\u20102320\u2010B\u2010002\u2010040"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 107\u20102320\u2010B\u2010002\u2010012"],"award-info":[{"award-number":["MOST 107\u20102320\u2010B\u2010002\u2010012"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Nuclear receptor interaction protein (NRIP) co\u2010localizes with acetylcholine receptor (AChR) at the neuromuscular junction (NMJ), and NRIP deficiency causes aberrant NMJ architecture. However, the normal physiological and pathophysiological roles of NRIP in NMJ are still unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We investigated the co\u2010localization and interaction of NRIP with AChR\u2010associated proteins using immunofluorescence and immunoprecipitation assay, respectively. The binding affinity of AChR\u2010associated proteins was analysed in muscle\u2010restricted NRIP knockout mice and NRIP knockout muscle cells (C2C12). We further collected the sera from 43 patients with myasthenia gravis (MG), an NMJ disorder. The existence and features of anti\u2010NRIP autoantibody in sera were studied using Western blot and epitope mapping.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      NRIP co\u2010localized with AChR, rapsyn and \u03b1\u2010actinin 2 (ACTN2) in gastrocnemius muscles of mice; and \u03b1\u2010bungarotoxin (BTX) pull\u2010down assay revealed NRIP with rapsyn and ACTN2 in complexes from muscle tissues and cells. NRIP directly binds with \u03b1 subunit of AChR (AChR\u03b1)\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      to affect the binding affinity of AChR with rapsyn and rapsyn with ACTN2. In 43 patients with MG (age, 58.4\u00a0\u00b1\u00a014.5\u00a0years; female, 55.8%), we detected six of them (14.0%) having anti\u2010NRIP autoantibody. The presence of anti\u2010NRIP autoantibody correlated with a more severe type of MG when AChR autoantibody existed (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.011). The higher the titre of anti\u2010NRIP autoantibody, the more severe MG severity (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.032). The main immunogenic region is likely on the IQ motif of NRIP. We also showed the IgG subclass of anti\u2010NRIP autoantibody mainly to be IgG1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>NRIP is a novel AChR\u03b1 binding protein and involves structural NMJ formation, which acts as a scaffold to stabilize AChR\u2013rapsyn\u2013ACTN2 complexes. Anti\u2010NRIP autoantibody is a novel autoantibody in MG and plays a detrimental role in MG with the coexistence of anti\u2010AChR autoantibody.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12697","type":"journal-article","created":{"date-parts":[[2021,3,27]],"date-time":"2021-03-27T01:53:50Z","timestamp":1616810030000},"page":"665-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Autoantibody of NRIP, a novel AChR\u2010interacting protein, plays a detrimental role in myasthenia gravis"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8420-6951","authenticated-orcid":false,"given":"Li\u2010Kai","family":"Tsai","sequence":"first","affiliation":[{"name":"Department of Neurology National Taiwan University Hospital  Taipei Taiwan"},{"name":"Department of Neurology National Taiwan University Hospital, Hsinchu Branch  Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I\u2010Hsin","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Microbiology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Chao","family":"Chao","sequence":"additional","affiliation":[{"name":"Department of Neurology National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsueh\u2010Wen","family":"Hsueh","sequence":"additional","affiliation":[{"name":"Department of Neurology National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin\u2010Hsiung","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Microbiology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Hsin","family":"Huang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Microbiology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong\u2010Wei","family":"Weng","sequence":"additional","affiliation":[{"name":"Graduate Institute of Microbiology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu\u2010Yun","family":"Lai","sequence":"additional","affiliation":[{"name":"Graduate Institute of Microbiology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Chieh","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Neurology National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeou\u2010Ping","family":"Tsao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Mackay Memorial Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Show\u2010Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Microbiology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm942"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412169200"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02453-10"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.174441"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-4-53"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12299"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1602678"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-019-0079-y"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(15)00145-3"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2016.44"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.55.1.16"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-022516-034255"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(05)80031-5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2508-08.2008"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-6606-1-18"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020490"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2019.09.009"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2833-09.2009"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13522"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8070671"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06808.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/ane.12271"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12501"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12697","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.12697","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12697","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T03:37:36Z","timestamp":1764041856000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.12697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,26]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1002\/jcsm.12697"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.12697","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,26]]},"assertion":[{"value":"2020-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}