{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:39:36Z","timestamp":1777423176952,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T00:00:00Z","timestamp":1620172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2021,6]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n In vivo<\/jats:italic>\n muscle protein synthesis rates are typically assessed by measuring the incorporation rate of stable isotope labelled amino acids in skeletal muscle tissue collected from\n vastus lateralis<\/jats:italic>\n muscle. It remains to be established whether muscle protein synthesis rates in the\n vastus lateralis<\/jats:italic>\n are representative of muscle protein synthesis rates of other muscle groups. We hypothesized that post\u2010absorptive muscle protein synthesis rates differ between\n vastus lateralis<\/jats:italic>\n and\n rectus abdominis<\/jats:italic>\n ,\n pectoralis major<\/jats:italic>\n , or\n temporalis<\/jats:italic>\n muscle\n in vivo<\/jats:italic>\n in humans.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Twenty\u2010four patients (62\u00a0\u00b1\u00a03\u00a0years, 42% female), scheduled to undergo surgery, participated in this study and underwent primed continuous intravenous infusions with\n l<\/jats:sc>\n \u2010[ring\u2010\n 13<\/jats:sup>\n C\n 6<\/jats:sub>\n ]\u2010phenylalanine. During the surgical procedures, serum samples were collected, and muscle tissue was obtained from the\n vastus lateralis<\/jats:italic>\n as well as from the\n rectus abdominis<\/jats:italic>\n ,\n pectoralis major<\/jats:italic>\n , or\n temporalis<\/jats:italic>\n muscle. Fractional mixed muscle protein synthesis rates (%\/h) were assessed by measuring the incorporation of\n l<\/jats:sc>\n \u2010[ring\u2010\n 13<\/jats:sup>\n C\n 6<\/jats:sub>\n ]\u2010phenylalanine into muscle tissue protein.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Serum\n l<\/jats:sc>\n \u2010[ring\u2010\n 13<\/jats:sup>\n C\n 6<\/jats:sub>\n ]\u2010phenylalanine enrichments did not change throughout the infusion period. Post\u2010absorptive muscle protein synthesis rates calculated based upon serum\n l<\/jats:sc>\n \u2010[ring\u2010\n 13<\/jats:sup>\n C\n 6<\/jats:sub>\n ]\u2010phenylalanine enrichments did not differ between\n vastus lateralis<\/jats:italic>\n and\n rectus abdominis<\/jats:italic>\n (0.032\u00a0\u00b1\u00a00.004 vs. 0.038\u00a0\u00b1\u00a00.003%\/h),\n vastus lateralis<\/jats:italic>\n and\n pectoralis major<\/jats:italic>\n , (0.025\u00a0\u00b1\u00a00.003 vs. 0.022\u00a0\u00b1\u00a00.005%\/h) or\n vastus lateralis<\/jats:italic>\n and\n temporalis<\/jats:italic>\n (0.047\u00a0\u00b1\u00a00.005 vs. 0.043\u00a0\u00b1\u00a00.005%\/h) muscle, respectively (\n P<\/jats:italic>\n \u00a0>\u00a00.05). When fractional muscle protein synthesis rates were calculated based upon tissue\u2010free\n l<\/jats:sc>\n \u2010[ring\u2010\n 13<\/jats:sup>\n C\n 6<\/jats:sub>\n ]\u2010phenylalanine enrichments as the preferred precursor pool, muscle protein synthesis rates were significantly higher in\n rectus abdominis<\/jats:italic>\n (0.089\u00a0\u00b1\u00a00.008%\/h) compared with\n vastus lateralis<\/jats:italic>\n (0.054\u00a0\u00b1\u00a00.005%\/h) muscle (\n P<\/jats:italic>\n \u00a0<\u00a00.01). No differences were observed between fractional muscle protein synthesis rates in\n vastus lateralis<\/jats:italic>\n and\n pectoralis major<\/jats:italic>\n (0.046\u00a0\u00b1\u00a00.003 vs. 0.041\u00a0\u00b1\u00a00.008%\/h) or\n vastus lateralis<\/jats:italic>\n and\n temporalis<\/jats:italic>\n (0.073\u00a0\u00b1\u00a00.008 vs. 0.083\u00a0\u00b1\u00a00.011%\/h) muscle, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Post\u2010absorptive muscle protein synthesis rates are higher in\n rectus abdominis<\/jats:italic>\n when compared with\n vastus lateralis<\/jats:italic>\n muscle. Post\u2010absorptive muscle protein synthesis rates do not differ between\n vastus lateralis<\/jats:italic>\n and\n pectoralis major<\/jats:italic>\n or\n temporalis<\/jats:italic>\n muscle. Protein synthesis rates in muscle tissue samples obtained during surgery do not necessarily represent a good proxy for appendicular skeletal muscle protein synthesis rates.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcsm.12701","type":"journal-article","created":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T05:27:23Z","timestamp":1620278843000},"page":"769-778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Basal protein synthesis rates differ between\n vastus lateralis<\/i>\n and\n rectus abdominis<\/i>\n muscle"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2877-0114","authenticated-orcid":false,"given":"Joey S.J.","family":"Smeets","sequence":"first","affiliation":[{"name":"Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4866-7368","authenticated-orcid":false,"given":"Astrid M.H.","family":"Horstman","sequence":"additional","affiliation":[{"name":"Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David P.J.","family":"van Dijk","sequence":"additional","affiliation":[{"name":"Department of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Astrid G.M.","family":"van Boxtel","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Catharina Hospital Eindhoven The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joost F.","family":"ter Woorst","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Catharina Hospital Eindhoven The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5202-9345","authenticated-orcid":false,"given":"Steven W.M. Olde","family":"Damink","sequence":"additional","affiliation":[{"name":"Department of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The Netherlands"},{"name":"Department of General, Visceral and Transplantation Surgery RWTH University Hospital Aachen Aachen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olaf E.M.G.","family":"Schijns","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Maastricht University Medical Centre+ Maastricht The Netherlands"},{"name":"School of Mental Health and Neuroscience (MHeNS) Maastricht University Maastricht The Netherlands"},{"name":"Academic Center for Epileptology, location Maastricht Maastricht University Medical Centre+ Maastricht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6768-9231","authenticated-orcid":false,"given":"Luc J.C.","family":"van 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