{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:10:44Z","timestamp":1792203044455,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T00:00:00Z","timestamp":1626393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>A reduction in the skeletal muscle mass worsens the prognosis of patients with various cancers. Our previous studies indicated that cisplatin administration to mice caused muscle atrophy. This is a concern for human patients receiving cisplatin. The insulin\u2010like growth factor 1 (IGF\u20101)\/phosphoinositide 3\u2010kinase (PI3K)\/Akt pathway stimulates the rate of protein synthesis in skeletal muscle. Thus, IGF\u2010I can be a central therapeutic target for preventing the loss of skeletal muscle mass in muscle atrophy, although it remains unclear whether pharmacological activation of the IGF\u20101\/PI3K\/Akt pathway attenuates muscle atrophy induced by cisplatin. In this study, we examined whether exogenous recombinant human IGF\u20101 attenuated cisplatin\u2010induced muscle atrophy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Male C57BL\/6J mice (8\u20139\u00a0weeks old) were injected with cisplatin or saline for four consecutive days. On Day 5, quadriceps muscles were isolated. Mecasermin (recombinant human IGF\u20101) or the vehicle control was subcutaneously administered 30\u00a0min prior to cisplatin administration. A dietary restriction group achieving weight loss equivalent to that caused by cisplatin administration was used as a second control. C2C12 myotubes were treated with cisplatin with\/without recombinant mouse IGF\u20101. The skeletal muscle protein synthesis\/degradation pathway was analysed by histological and biochemical methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Cisplatin reduced protein level of IGF\u20101 by about 85% compared with the vehicle group and also reduced IGF\u20101\/PI3K\/Akt signalling in skeletal muscle. Under this condition, the protein levels of muscle ring finger protein 1 (MuRF1) and atrophy gene 1 (atrogin\u20101) were increased in quadriceps muscles (MuRF1; 3.0\u00a0\u00b1\u00a00.1 folds, atrogin\u20101; 3.0\u00a0\u00b1\u00a00.3 folds,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001, respectively). The administration of a combination of cisplatin and IGF\u20101 significantly suppressed the cisplatin\u2010induced downregulation of IGF\u20101\/PI3K\/Akt signalling and upregulation of MuRF1 and atrogin\u20101 (up to 1.6\u00a0\u00b1\u00a00.3 and 1.5\u00a0\u00b1\u00a00.4 folds,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001, respectively), resulting in diminished muscular atrophy. IGF\u20101 showed similar effects in cisplatin\u2010treated C2C12 myotubes, as well as the quadriceps muscle in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The downregulation of IGF\u20101 expression in skeletal muscle might be one of the factors playing an important role in the development of cisplatin\u2010induced muscular atrophy. Compensating for this downregulation with exogenous IGF\u20101 suggests that it could be a therapeutic target for limiting the loss of skeletal muscle mass in cisplatin\u2010induced muscle atrophy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12760","type":"journal-article","created":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T04:47:33Z","timestamp":1626410853000},"page":"1570-1581","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Exogenous insulin\u2010like growth factor 1 attenuates cisplatin\u2010induced muscle atrophy in mice"],"prefix":"10.1002","volume":"12","author":[{"given":"Hiroyasu","family":"Sakai","sequence":"first","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maho","family":"Asami","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoko","family":"Kitora","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Miyauchi","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rei","family":"Tachinooka","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risako","family":"Kon","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobutomo","family":"Ikarashi","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Chiba","sequence":"additional","affiliation":[{"name":"Department of Physiology and Molecular Sciences, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junzo","family":"Kamei","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Pharmacology, School of Pharmacy Hoshi University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/84.3.475"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140403"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-011-1976-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.02.031"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2018.0137"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065874"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251541198"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00204.2014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2014.05.001"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40279-017-0845-5"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/2044-5040-1-4"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1101-1009"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.142166599"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/82_2010_78"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9091970"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.567"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2672"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.13024"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2004.11.001"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12954"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2013.09.005"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.017"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.09.020"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.06.009"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-017-2045-4"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00400-3"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.010389"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7670"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.09.006"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00105.2009"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00389.2012"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2013.10.001"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.20.12109"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1998.275.3.E373"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0289fje"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1594"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.07.011"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2020.12.007"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2020.583877"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0205-4"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12501"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12760","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.12760","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.12760","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T00:59:25Z","timestamp":1693184365000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.12760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,16]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/jcsm.12760"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.12760","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,16]]},"assertion":[{"value":"2020-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}