{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:50:18Z","timestamp":1793062218544,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST\u2010109\u20102320\u2010B\u2010038\u2010058\u2010MY3"],"award-info":[{"award-number":["MOST\u2010109\u20102320\u2010B\u2010038\u2010058\u2010MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Age\u2010related muscle dysfunctions are common disorders resulting in poor quality of life in the elderly. Probiotic supplementation is a potential strategy for preventing age\u2010related sarcopenia as evidence suggests that probiotics can enhance muscle function via the gut\u2013muscle axis. However, the effects and mechanisms of probiotics in age\u2010related sarcopenia are currently unknown. In this study, we examined the effects of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Lactobacillus casei<\/jats:italic>\n                      <\/jats:styled-content>\n                      Shirota (LcS), a probiotic previously reported to improve muscle function in young adult mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We administered LcS (1\u00a0\u00d7\u00a010\n                      <jats:sup>8<\/jats:sup>\n                      or 1\u00a0\u00d7\u00a010\n                      <jats:sup>9<\/jats:sup>\n                      \u00a0CFU\/mouse\/day) by oral gavage to senescence\u2010accelerated mouse prone\u20108 mice for 12\u00a0weeks (16\u2010 to 28\u2010week\u2010old). Sixteen\u2010week\u2010old and 28\u2010week\u2010old SMAP8 mice were included as non\u2010aged and aged controls, respectively. Muscle condition was evaluated using dual\u2010energy X\u2010ray absorptiometry for muscle mass, holding impulse and grip strength tests for muscle strength, and oxygen consumption rate, gene expressions of mitochondrial biogenesis, and mitochondrial number assays for mitochondria function. Inflammatory cytokines were determined using enzyme\u2010linked immunosorbent assay. Gas chromatography\u2013mass spectrometry was utilized to measure the short\u2010chain fatty acid levels. The gut microbiota was analysed based on the data of 16S rRNA gene sequencing of mouse stool.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The LcS supplementation reduced age\u2010related declines in muscle mass (&gt;94.6%,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.04), strength (&gt;66% in holding impulse and &gt;96.3% in grip strength,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), and mitochondrial function (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). The concentration of short\u2010chain fatty acids (acetic, isobutyric, butyric, penic, and hexanoic acid) was recovered by LcS (&gt;65.9% in the mice given high dose of LcS,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) in the aged mice, and LcS attenuated age\u2010related increases in inflammation (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and reactive oxygen species (&gt;89.4%,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001). The high dose of LcS supplementation was also associated with distinct microbiota composition as indicated by the separation of groups in the beta\u2010diversity analysis (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.027). LcS supplementation altered predicted bacterial functions based on the gut microbiota. Apoptosis (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.026), p53 signalling (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.017), and non\u2010homologous end\u2010joining (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.031) were significantly reduced, whereas DNA repair and recombination proteins (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.043), RNA polymerase (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.008), and aminoacyl\u2010tRNA biosynthesis (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.003) were increased. Finally, the genera enriched by high\u2010dose LcS [linear discriminant analysis (LDA) score\u00a0&gt;\u00a02.0] were positively correlated with healthy muscle and physiological condition (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), while the genera enriched in aged control mice (LDA score\u00a0&gt;\u00a02.0) were negatively associated with healthy muscle and physiological condition (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Lactobacillus casei<\/jats:italic>\n                      <\/jats:styled-content>\n                      Shirota represents an active modulator that regulates the onset and progression of age\u2010related muscle impairment potentially via the gut\u2013muscle axis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12849","type":"journal-article","created":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T00:47:52Z","timestamp":1636678072000},"page":"515-531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":161,"title":["Probiotic supplementation attenuates age\u2010related sarcopenia via the gut\u2013muscle axis in SAMP8 mice"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4789-3155","authenticated-orcid":false,"given":"Li\u2010Han","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Fisheries Science National Taiwan University  Taipei Taiwan"},{"name":"Department of Life Science National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shy\u2010Shin","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Family Medicine, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"Department of Family Medicine Taipei Medical University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin\u2010Yi","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition Taipei Medical University  Taipei Taiwan"},{"name":"Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology Taipei Medical University  Taipei Taiwan"},{"name":"Master Program in Clinical Pharmacogenomics and Pharmacoproteomics, College of Pharmacy Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chieh\u2010Hsi","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Hsu","family":"Pan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Taipei Medical University  Taipei Taiwan"},{"name":"Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Chao","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine Taipei Medical University Hospital  Taipei Taiwan"},{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Hung","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology Tatung University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition Taipei Medical University  Taipei 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