{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:40:46Z","timestamp":1787668846204,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/10089\u20102"],"award-info":[{"award-number":["2018\/10089\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/06517\u20101"],"award-info":[{"award-number":["2019\/06517\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Fetal stage is a critical developmental window for the skeletal muscle, but little information is available about the impact of maternal vitamin D (Vit. D) deficiency (VDD) on offspring lean mass development in the adult life of male and female animals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Female rats (Wistar Hannover) were fed either a control (1000\u00a0IU Vit. D3\/kg) or a VDD diet (0\u00a0IU Vit. D3\/kg) for 6\u00a0weeks and during gestation and lactation. At weaning, male and female offspring were randomly separated and received a standard diet up to 180\u00a0days old.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Vitamin D deficiency induced muscle atrophy in the male (M\u2010VDD) offspring at the end of weaning, an effect that was reverted along the time. Following 180\u00a0days, fast\u2010twitch skeletal muscles [\n                      <jats:italic>extensor digitorum longus<\/jats:italic>\n                      (EDL)] from the M\u2010VDD showed a decrease (20%;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) in the number of total fibres but an increase in the cross\u2010sectional area of IIB (17%;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), IIA (19%;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and IIAX (21%;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) fibres. The fibre hypertrophy was associated with the higher protein levels of MyoD (73%;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and myogenin (55% %;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and in the number of satellite cells (128.8\u00a0\u00b1\u00a014 vs. 91\u00a0\u00b1\u00a07.6 nuclei Pax7\u00a0+\u00a0in the M\u2010CTRL;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). M\u2010VDD increased time to fatigue during\n                      <jats:italic>ex vivo<\/jats:italic>\n                      contractions of EDL muscles and showed an increase in the phosphorylation levels of IGF\u20101\/insulin receptor and their downstream targets related to anabolic processes and myogenic activation, including Ser\n                      <jats:sup>473<\/jats:sup>\n                      Akt and Ser\n                      <jats:sup>21\/9<\/jats:sup>\n                      GSK\u20103\u03b2. In such muscles, maternal VDD induced a compensatory increase in the content of calcitriol (two\u2010fold;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and CYP27B1 (58%;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), a metabolizing enzyme that converts calcidiol to calcitriol. Interestingly, most morphological and biochemical changes found in EDL were not observed in slow\u2010twitch skeletal muscles (soleus) from the M\u2010VDD group as well as in both EDL and soleus muscles from the female offspring.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These data show that maternal VDD selectively affects the development of type\u2010II muscle fibres in male offspring rats but not in female offspring rats and suggest that the enhancement of their size and fatigue resistance in fast\u2010twitch skeletal muscle (EDL) is probably due to a compensatory increase in the muscle content of Vit. D in the adult age.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.12986","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T04:54:59Z","timestamp":1652849699000},"page":"2175-2187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0391-7255","authenticated-orcid":false,"given":"Natany G.","family":"Reis","sequence":"first","affiliation":[{"name":"Department of Physiology Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5738-9374","authenticated-orcid":false,"given":"Ana 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