{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:56:40Z","timestamp":1790211400558,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:00:00Z","timestamp":1664150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Brazilian green propolis is an important honeybee product that is considered beneficial for health. Here, we examined the therapeutic potential of dietary supplementation with propolis against sarcopenic obesity using Db\/Db mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Db\/m mice fed a normal diet alone and Db\/Db mice fed normal diet alone, or supplemented with different amounts of propolis (0.08, 0.4 and 2%), were examined for effects on sarcopenic obesity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Propolis improved the glucose tolerance (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), increased the grip strength (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and the weight of soleus (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.006) and plantaris muscles (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.008). Moreover, propolis improved the non\u2010alcoholic fatty liver disease activity score (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and decreased the expression of genes related to inflammation, liver fibrosis and fatty acid metabolism. Propolis decreased the accumulation of saturated fatty acids in the liver and increased their excretion in faeces. With regard to the innate immunity, propolis decreased the ratio of M1 macrophages (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.008) and Type 1 and 3 innate lymphoid cells to CD45\u2010positive cells (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) and increased the ratio of M2 macrophages (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.002) and ILC2s (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.007) in the liver. Additionally, propolis decreased the expression of genes related to muscle atrophy and inflammation and the concentration of saturated fatty acids in the soleus muscle. 16S rRNA phylogenetic sequencing revealed that propolis increased the Bacteroidetes\/Firmicutes ratio, and the abundance of Butyricicoccus and Acetivibrio genera. Gut microbiota related to the pentose phosphatase pathway and glycerolipid metabolism was more prevalent after the administration of propolis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This is the first study to demonstrate that propolis can improve sarcopenic obesity by improving dysbiosis due to overeating and provides new insights into diet\u2013microbiota interactions during sarcopenic obesity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13076","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T20:18:55Z","timestamp":1664223535000},"page":"3028-3047","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Brazilian green propolis improves gut microbiota dysbiosis and protects against sarcopenic obesity"],"prefix":"10.1002","volume":"13","author":[{"given":"Takuro","family":"Okamura","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahide","family":"Hamaguchi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Bamba","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanako","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonori","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saori","family":"Majima","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takafumi","family":"Senmaru","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emi","family":"Ushigome","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichiro","family":"Nishimoto","sequence":"additional","affiliation":[{"name":"Metabologenomics Inc.  Tsuruoka Yamagata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuji","family":"Yamada","sequence":"additional","affiliation":[{"name":"Metabologenomics Inc.  Tsuruoka Yamagata Japan"},{"name":"Department of Life Science and Technology Tokyo Institute of Technology  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chizuru","family":"Fujikura","sequence":"additional","affiliation":[{"name":"Institute for Bee Products and Health Science, R&amp;D Department Yamada Bee Company, Inc  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Asama","sequence":"additional","affiliation":[{"name":"Institute for Bee Products and Health Science, R&amp;D Department Yamada Bee Company, Inc  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuaki","family":"Okumura","sequence":"additional","affiliation":[{"name":"Institute for Bee Products and Health Science, R&amp;D Department Yamada Bee Company, Inc  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Takakuwa","sequence":"additional","affiliation":[{"name":"Agilent Technologies, Chromatography Mass Spectrometry Sales Department Life Science and Applied Markets Group  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoichi","family":"Sasano","sequence":"additional","affiliation":[{"name":"AiSTI SCIENCE CO., Ltd  Wakayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0903-1797","authenticated-orcid":false,"given":"Michiaki","family":"Fukui","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2017.151"},{"key":"e_1_2_8_3_1","first-page":"477","article-title":"Shortage of energy intake rather than protein intake is associated with sarcopenia in elderly patients with type 2 diabetes: A cross\u2010sectional study of the KAMOGAWA\u2010DM cohort","volume":"67","author":"Okamura T","year":"2018","journal-title":"J Diabetes"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.18-10"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.EJ16-0124"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc10-0107"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11102291"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/1259510"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-1305-8"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/br.2015.436"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.27.1797"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods9101362"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0FO02541K"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900747"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31353-1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20701"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.36.343"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2005.11.007"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.2741\/e418"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1500.1998.00020.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119133"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2012.06.008"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.03.034"},{"key":"e_1_2_8_24_1","first-page":"789","article-title":"Mitochondrial dysfunction results from oxidative stress in the skeletal muscle of diet\u2010induced insulin\u2010resistant mice","volume":"118","author":"Bonnard C","year":"2008","journal-title":"J Clin Investig"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.669672"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.02.010"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2013.02.004"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.03.017"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109393"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2653"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2015.06.011"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2017.66"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2014.08.010"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-30-1-179"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2015.07.026"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1937"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0368z.x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00310.2019"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2014.03.017"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01260-12"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00009.2005"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2006.02.005"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12899"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13076","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.13076","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T08:00:24Z","timestamp":1754640024000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.13076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,26]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/jcsm.13076"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.13076","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,26]]},"assertion":[{"value":"2021-09-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}