{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:50:46Z","timestamp":1791474646986,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vitamin D is an essential nutrient in musculoskeletal function; however, its relationship to sarcopenia remains ambiguous, and the mechanisms and targets of vitamin D activity have not been elucidated. This study aimed to clarify the role of vitamin D in mature skeletal muscle and its relationship with sarcopenia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This epidemiological study included 1653 community residents who participated in both the fifth and seventh waves of the National Institute for Longevity Sciences, Longitudinal Study of Aging and had complete background data. Participants were classified into two groups: vitamin D\u2010deficient (serum 25\u2010hydroxyvitamin D\u00a0&lt;\u00a020\u00a0ng\/mL) and non\u2010deficient (serum 25\u2010hydroxyvitamin D\u00a0\u2265\u00a020\u00a0ng\/mL); they underwent propensity\u2010score matching for background factors (age, sex, height, weight, comorbidities, smoker, alcohol intake, energy intake, vitamin D intake, steps, activity, season and sarcopenia). Changes in muscle strength and mass over the 4\u2010year period were compared. For basic analysis, we generated\n                      <jats:italic>Myf6<\/jats:italic>\n                      <jats:sup>CreERT2<\/jats:sup>\n                      Vitamin D Receptor (VDR)\u2010floxed (\n                      <jats:italic>Vdr<\/jats:italic>\n                      <jats:sup>mcKO<\/jats:sup>\n                      ) mice with mature muscle fibre\u2010specific vitamin D receptor knockout, injected tamoxifen into 8\u2010week\u2010old mice and analysed various phenotypes at 16\u00a0weeks of age.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Grip strength reduction was significantly greater in the deficient group (\u22121.55\u00a0\u00b1\u00a02.47\u00a0kg) than in the non\u2010deficient group (\u22121.13\u00a0\u00b1\u00a02.47\u00a0kg;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.019). Appendicular skeletal muscle mass reduction did not differ significantly between deficient (\u22120.05\u00a0\u00b1\u00a00.79\u00a0kg) and non\u2010deficient (\u22120.01\u00a0\u00b1\u00a00.74\u00a0kg) groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.423). The incidence of new cases of sarcopenia was significantly higher in the deficient group (15 vs. 5 cases;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.039). Skeletal muscle phenotyping of\n                      <jats:italic>Vdr<\/jats:italic>\n                      <jats:sup>mcKO<\/jats:sup>\n                      mice showed no significant differences in muscle weight, myofibre percentage or myofibre cross\u2010sectional area; however, both forelimb and four\u2010limb muscle strength were significantly lower in\n                      <jats:italic>Vdr<\/jats:italic>\n                      <jats:sup>mcKO<\/jats:sup>\n                      mice (males: forelimb,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.048; four\u2010limb,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.029; females: forelimb,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001; four\u2010limb,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001). Expression profiling revealed a significant decrease in expression of sarcoendoplasmic reticulum Ca\n                      <jats:sup>2+<\/jats:sup>\n                      \u2010ATPase (SERCA) 1 (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.019) and SERCA2a (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.049) genes in the\n                      <jats:italic>Vdr<\/jats:italic>\n                      <jats:sup>mcKO<\/jats:sup>\n                      mice. In contrast, expression of non\u2010muscle SERCA2b and myoregulin genes showed no changes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Vitamin D deficiency affects muscle strength and may contribute to the onset of sarcopenia. Vitamin D\u2010VDR signalling has minimal influence on the regulation of muscle mass in mature myofibres but has a significant influence on muscle strength.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13102","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T01:56:39Z","timestamp":1665712599000},"page":"2961-2973","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Influence of vitamin D on sarcopenia pathophysiology: A longitudinal study in humans and basic research in knockout mice"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7843-594X","authenticated-orcid":false,"given":"Takafumi","family":"Mizuno","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Department of Musculoskeletal Disease Geroscience Research Center, National Center for Geriatrics and Gerontology  Obu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1825-6923","authenticated-orcid":false,"given":"Tohru","family":"Hosoyama","sequence":"additional","affiliation":[{"name":"Department of Musculoskeletal Disease Geroscience Research Center, National Center for Geriatrics and Gerontology  Obu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makiko","family":"Tomida","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Aging National Center for Geriatrics and Gerontology  Obu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Nakamichi","sequence":"additional","affiliation":[{"name":"Institute for Oral Science Matsumoto Dental University  Nagano Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeaki","family":"Kato","sequence":"additional","affiliation":[{"name":"Graduate School of Life Science and Engineering Iryo Sosei University  Fukushima Japan"},{"name":"Research Institute of Innovative Medicine Tokiwa Foundation  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minako","family":"Kawai\u2010Takaishi","sequence":"additional","affiliation":[{"name":"Department of Musculoskeletal Disease Geroscience Research Center, National Center for Geriatrics and Gerontology  Obu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1877-9502","authenticated-orcid":false,"given":"Shinya","family":"Ishizuka","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Nishita","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Aging National Center for Geriatrics and Gerontology  Obu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chikako","family":"Tange","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Aging National Center for Geriatrics and Gerontology  Obu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Shimokata","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Aging National Center for Geriatrics and Gerontology  Obu Japan"},{"name":"Graduate School of Nutritional Sciences Nagoya University of Arts and Sciences  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Imagama","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6184-570X","authenticated-orcid":false,"given":"Rei","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Aging National Center for Geriatrics and Gerontology  Obu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-009-0954-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-1698"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-030604"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/80.3.752"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.b3692"},{"key":"e_1_2_8_7_1","first-page":"859","article-title":"Effect of vitamin D 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