{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T15:54:35Z","timestamp":1784735675334,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003981","name":"Agenzia Spaziale Italiana","doi-asserted-by":"publisher","award":["DC\u2010VUM\u20102017\u2010006"],"award-info":[{"award-number":["DC\u2010VUM\u20102017\u2010006"]}],"id":[{"id":"10.13039\/501100003981","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001706","name":"Louis-Jeantet Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001706","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011102","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["HEALTH\u2010F4\u20102008\u2010201648\/PROSPECTS"],"award-info":[{"award-number":["HEALTH\u2010F4\u20102008\u2010201648\/PROSPECTS"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Inactivity and unloading induce skeletal muscle atrophy, loss of strength and detrimental metabolic effects. Bed rest is a model to study the impact of inactivity on the musculoskeletal system. It not only provides information for bed\u2010ridden patients care, but it is also a ground\u2010based spaceflight analogue used to mimic the challenges of long space missions for the human body. In both cases, it would be desirable to develop a panel of biomarkers to monitor muscle atrophy in a minimally invasive way at point of care to limit the onset of muscle loss in a personalized fashion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We applied mass spectrometry\u2010based proteomics to measure plasma protein abundance changes in response to 10\u00a0days of bed rest in 10 young males. To validate the correlation between muscle atrophy and the significant hits emerging from our study, we analysed in parallel, with the same pipeline, a cohort of cancer patients with or without cachexia and age\u2010matched controls. Our analysis resulted in the quantification of over 500 proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Unloading affected plasma concentration of proteins of the complement cascade, lipid carriers and proteins derived from tissue leakage. Among the latter, teneurin\u20104 increased 1.6\u2010fold in plasma at bed rest day 10 (BR10) compared with BR0 (6.E9 vs. 4.3E9, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.02) and decreased to 0.6\u2010fold the initial abundance after 2\u00a0days of recovery at normal daily activity (R\u00a0+\u00a02, 2.7E9, <jats:italic>P<\/jats:italic>\u00a0=\u00a03.3E\u20104); the extracellular matrix protein lumican was decreased to 0.7\u2010fold (1.2E9 vs. 8.5E8, <jats:italic>P<\/jats:italic>\u00a0=\u00a01.5E\u20104) at BR10 and remained as low at R\u00a0+\u00a02. We identified six proteins distinguishing subjects developing unloading\u2010mediated muscle atrophy (decrease of &gt;4% of quadriceps cross\u2010sectional area) from those largely maintaining their initial muscle mass. Among them, transthyretin, a thyroid hormone\u2010binding protein, was significantly less abundant at BR10 in the plasma of subjects with muscle atrophy compared with those with no atrophy (1.6E10 vs. 2.6E10, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.001). Haptoglobin\u2010related protein was also significantly reduced in the serum of cancer patients with cachexia compared with that of controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings highlight a combination or proteomic changes that can be explored as potential biomarkers of muscle atrophy occurring under different conditions. The panel of significant proteomic differences distinguishing atrophy\u2010prone and atrophy\u2010resistant subjects after 10\u00a0days of bed rest need to be tested in a larger cohort to validate their potential to predict inactivity\u2010triggered muscle loss in humans.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13146","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T03:02:12Z","timestamp":1671073332000},"page":"439-451","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Plasma proteome profiling of healthy subjects undergoing bed rest reveals unloading\u2010dependent changes linked to muscle atrophy"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6244-3358","authenticated-orcid":false,"given":"Marta","family":"Murgia","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padua 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