{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:18:31Z","timestamp":1792801111108,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Rhabdomyosarcoma (RMS) is an aggressive soft tissue sarcoma that most often develops in children. Chemoradiation therapy is a standard treatment modality; however, the detrimental long\u2010term skeletal muscle consequences of this therapy in juvenile cancer survivors include muscle atrophy and fibrosis resulting in decreased physical performance. Using a novel model of murine resistance\u00a0and endurance exercise\u00a0training, we investigate its role in preventing the long\u2010term effects of juvenile RMS plus therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four\u2010week\u2010old male (<jats:italic>n<\/jats:italic>\u00a0=\u00a010) and female (<jats:italic>n<\/jats:italic>\u00a0=\u00a010) C57Bl\/6J mice were injected with M3\u20109\u2010M RMS cell into the left gastrocnemius with the right limb serving as an internal control (CON). Mice received a systemic vincristine injection and then five doses of 4.8\u00a0Gy of gamma radiation localized to the left hindlimb (RMS\u00a0+\u00a0Tx). Mice were then randomly divided into either sedentary (SED) or resistance\u00a0and endurance exercise\u00a0training (RET) groups. Changes in exercise performance, body composition, myocellular adaptations and the inflammatory\/fibrotic transcriptome were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RET improved endurance performance (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) and body composition (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0004) compared to SED. RMS\u00a0+\u00a0Tx resulted in significantly lower muscle weight (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.015) and significantly smaller myofibre cross\u2010sectional area (CSA) (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.014). Conversely, RET resulted in significantly higher muscle weight (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.030) and significantly larger Type IIA (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.014) and IIB (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.015) fibre CSA. RMS\u00a0+\u00a0Tx resulted in significantly more muscle fibrosis (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.028), which was not prevented by RET. RMS\u00a0+\u00a0Tx resulted in significantly fewer mononuclear cells (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and muscle satellite (stem) cells (MuSCs) (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and significantly more immune cells (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) than CON. RET resulted in significantly more fibro\u2010adipogenic progenitors (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), a trend for more MuSCs (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.076) than SED and significantly more endothelial cells specifically in the RMS\u00a0+\u00a0Tx limb. Transcriptomic changes revealed significantly higher expression of inflammatory and fibrotic genes in RMS\u00a0+\u00a0Tx, which was prevented by RET. In the RMS\u00a0+\u00a0Tx model, RET also significantly altered expression of genes involved in extracellular matrix turnover.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study suggests that RET preserves muscle mass and performance in a model of juvenile RMS survivorship while partially restoring cellular dynamics and the inflammatory and fibrotic transcriptome.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13185","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T02:13:16Z","timestamp":1676599996000},"page":"781-793","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Resistance and\u00a0endurance exercise\u00a0training improves muscle mass and the inflammatory\/fibrotic transcriptome in a rhabdomyosarcoma model"],"prefix":"10.1002","volume":"14","author":[{"given":"Nicolas","family":"Collao","sequence":"first","affiliation":[{"name":"School of Human Kinetics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Sanders","sequence":"additional","affiliation":[{"name":"School of Human Kinetics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Caminiti","sequence":"additional","affiliation":[{"name":"School of Human Kinetics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Messeiller","sequence":"additional","affiliation":[{"name":"School of Human Kinetics University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6462-2955","authenticated-orcid":false,"given":"Michael","family":"De Lisio","sequence":"additional","affiliation":[{"name":"School of Human Kinetics University of Ottawa  Ottawa Ontario Canada"},{"name":"Department of Cellular and Molecular Medicine, Regenerative Medicine Program University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.24387"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2004.02.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmrj.2011.08.535"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101760"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-75913-w"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12902"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.9.2.493"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.167197"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2017.08.003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR14473.1"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP274361"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000002116"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13395-022-00291-y"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21797"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-43204-8"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00050.2019"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.23048"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000001894"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000002711"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1139\/apnm-2020-0956"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2013.10.005"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-019-0278-8"},{"key":"e_1_2_8_24_1","unstructured":"HennigC.CRAN\u2014package fpc.https:\/\/cran.rproject.org\/web\/packages\/fpc\/index.html"},{"key":"e_1_2_8_25_1","unstructured":"AlexaA RahnenfuhrerJ.topGO: enrichment analysis for Gene Ontology. 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