{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T16:09:16Z","timestamp":1776182956173,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,3,5]],"date-time":"2023-03-05T00:00:00Z","timestamp":1677974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ014155052019"],"award-info":[{"award-number":["PJ014155052019"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2022R1C1C1004134"],"award-info":[{"award-number":["2022R1C1C1004134"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The improvement of colitis symptoms by treatment with IL\u201012\/23 p40 neutralizing antibody should increase the muscle mass and the function of the sarcopenia phenotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An experimental colitis model was induced by oral administration of 2% dextran sulfate sodium (DSS) for 7\u00a0days. During induction of colitis, IL\u201012\/23 p40 neutralizing antibody was injected twice on Days 3 and 5. The total body mass index was measured by dual\u2010energy X\u2010ray absorptiometry. The muscle function was measured by forelimb grip strength and fatigue running distance. The muscle fibre cross\u2010sectional area (CSA) was calculated after the transverse section and haematoxylin and eosin staining, and gene expression was confirmed by RT\u2010qPCR. Differentiated C2C12 cells were used as in vitro models and treated with recombinant IL12\/23 proteins to mimic the enhanced cytokines in colitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The symptoms of colitis were alleviated by injection of IL\u201012\/23 p40 neutralizing antibody compared with phosphate\u2010buffered saline (PBS), and the disease activity index score was significantly lower on Day 8 (0.0\u00a0\u00b1\u00a00.00 of cont. vs. 11.3\u00a0\u00b1\u00a00.9 of DSS\u00a0+\u00a0PBS, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001; DSS\u00a0+\u00a0PBS vs. 7.7\u00a0\u00b1\u00a01.25 of DSS\u00a0+\u00a0p40Ab, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001). The CSA of the gastrocnemius and tibialis anterior muscle fibres decreased in mice with DSS\u2010induced colitis (gastrocnemius, 1258.2 \u03bcm<jats:sup>2<\/jats:sup> \u00b1 176.45 of cont. vs. 640.1 \u03bcm<jats:sup>2<\/jats:sup> \u00b1 59.83 of DSS + PBS, <jats:italic>P<\/jats:italic> &lt; 0.0001; tibialis anterior, 1251.8 \u03bcm<jats:sup>2<\/jats:sup> \u00b1 331.48 of cont. vs. 678.9 \u03bcm<jats:sup>2<\/jats:sup> \u00b1 67.59 of DSS + PBS, <jats:italic>P<\/jats:italic> &lt; 0.0001), and the treatment of IL\u201012\/23 p40 neutralizing antibody partially restored CSA of the gastrocnemius (640.1 \u03bcm<jats:sup>2<\/jats:sup> \u00b1 59.83 of DSS + PBS vs. 1062.0 \u03bcm<jats:sup>2<\/jats:sup> \u00b1 83.41 of DSS + p40Ab, <jats:italic>P<\/jats:italic> &lt; 0.0001) and tibialis anterior (678.9 \u03bcm<jats:sup>2<\/jats:sup> \u00b1 67.59 of DSS + PBS vs. 1105.3 \u03bcm<jats:sup>2<\/jats:sup> \u00b1 143.15 of DSS + p40Ab, <jats:italic>P<\/jats:italic> = 0.0003).vs. 640.1\u00a0\u03bcm<jats:sup>2<\/jats:sup>\u00a0\u00b1\u00a059.83 of DSS\u00a0+\u00a0PBS, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) and tibialis anterior (1251.8\u00a0\u03bcm<jats:sup>2<\/jats:sup>\u00a0\u00b1\u00a0331.48 of cont. vs. 678.9\u00a0\u03bcm<jats:sup>2<\/jats:sup>\u00a0\u00b1\u00a067.59 of DSS\u00a0+\u00a0PBS, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001), and the treatment of IL\u201012\/23 p40 neutralizing antibody partially restored CSA of the gastrocnemius (640.1\u00a0\u03bcm<jats:sup>2<\/jats:sup>\u00a0\u00b1\u00a059.83 of DSS\u00a0+\u00a0PBS vs. 1062.0\u00a0\u03bcm<jats:sup>2<\/jats:sup>\u00a0\u00b1\u00a083.41 of DSS\u00a0+\u00a0p40Ab, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) and tibialis anterior (678.9\u00a0\u03bcm<jats:sup>2<\/jats:sup>\u00a0\u00b1\u00a067.59 of DSS\u00a0+\u00a0PBS vs. 1105.3\u00a0\u03bcm<jats:sup>2<\/jats:sup>\u00a0\u00b1\u00a0143.15 of DSS\u00a0+\u00a0p40Ab, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0003). In the evaluation of muscle function, grip strength and fatigue distance decreased by colitis were partially restored (grip strength: 139.9\u00a0g\u00a0\u00b1\u00a05.38 of cont. vs. 83.9\u00a0g\u00a0\u00b1\u00a05.48 of DSS\u00a0+\u00a0PBS, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001; DSS\u00a0+\u00a0PBS vs. 118.6\u00a0g\u00a0\u00b1\u00a04.05 of DSS\u00a0+\u00a0p40Ab, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001; fatigue distance: 872.5\u00a0m\u00a0\u00b1\u00a0104.01 of cont. vs. 58.2\u00a0m\u00a0\u00b1\u00a0107.72 of DSS\u00a0+\u00a0PBS, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001; DSS\u00a0+\u00a0PBS vs. 328.0\u00a0m\u00a0\u00b1\u00a0109.71 of DSS\u00a0+\u00a0p40Ab, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0015) by injection of IL\u201012\/23 p40 neutralizing antibody.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study demonstrates that Il\u201012\/23 acts directly on muscle to induce atrophy, and the IL\u201012\/23 p40 neutralizing antibody is effective not only in suppressing colitis but also in maintaining muscle mass and improving muscle function in an experimental colitis model.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13208","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T06:26:16Z","timestamp":1678083976000},"page":"1096-1106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The protective effect of IL\u201012\/23 neutralizing antibody in sarcopenia associated with dextran sulfate sodium\u2010induced experimental colitis"],"prefix":"10.1002","volume":"14","author":[{"given":"Youn\u2010Kwan","family":"Jung","sequence":"first","affiliation":[{"name":"Biomedical Research Institute Gyeongsang National University Hospital  Jinju Republic of 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