{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T05:48:03Z","timestamp":1792129683115,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102022R1A2C1003661"],"award-info":[{"award-number":["NRF\u20102022R1A2C1003661"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102022R1A2C1007901"],"award-info":[{"award-number":["NRF\u20102022R1A2C1007901"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102022R1C1C1002929"],"award-info":[{"award-number":["NRF\u20102022R1C1C1002929"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sarcopenia is characterized by a progressive decrease in skeletal muscle mass and function with age. Given that sarcopenia is associated with various metabolic disorders, effective metabolic biomarkers for its early detection are required. We aimed to investigate the metabolic biomarkers related to sarcopenia in elderly men and perform experimental studies using metabolomics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma metabolites from 142 elderly men, comprising a sarcopenia group and an age\u2010matched control group, were measured using global metabolome profiling. Muscle and plasma samples from an aging mouse model of sarcopenia, as well as cell media and cell lysates during myoblast differentiation, were analysed based on targeted metabolome profiling. Based on these experimental results, fatty acid amides were quantified from human plasma as well as human muscle tissues. The association of fatty acid amide levels with sarcopenia parameters was evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Global metabolome profiling showed that fatty acid amide levels were significantly different in the plasma of elderly men with sarcopenia (all <jats:italic>P<\/jats:italic>s\u00a0&lt;\u00a00.01). Consistent with these results in human plasma, targeted metabolome profiling in an aging mouse model of sarcopenia showed decreased levels of fatty acid amides in plasma but not in muscle tissue. In addition, the levels of fatty acid amides increased in cell lysates during muscle cell differentiation. Targeted metabolome profiling in men showed decreased docosahexaenoic acid ethanolamide (DHA EA) levels in the plasma (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.016) but not in the muscle of men with sarcopenia. DHA EA level was positively correlated with sarcopenia parameters such as skeletal muscle mass index (SMI) and handgrip strength (HGS) (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.001, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.001, respectively). The area under the receiver\u2010operating characteristic curve (AUC) for DHA EA level\u00a0\u2264\u00a04.60 fmol\/\u03bcL for sarcopenia was 0.618 (95% confidence interval [CI]: 0.532\u20130.698). DHA EA level\u00a0\u2264\u00a04.60 fmol\/\u03bcL was associated with a significantly greater likelihood of sarcopenia (odds ratio [OR]: 2.11, 95% CI: 1.03\u20134.30), independent of HGS. The addition of DHA EA level to age and HGS significantly improved the AUC from 0.620 to 0.691 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0497).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study demonstrated that fatty acid amides are potential circulating biomarkers in elderly men with sarcopenia. DHA EA, in particular, strongly related to muscle mass and strength, can be a key metabolite to become a reliable metabolic biomarker for sarcopenia. Further research on fatty acid amides will provide insights into the metabolomic changes relevant to sarcopenia from an aging perspective.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13244","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:05:05Z","timestamp":1682985905000},"page":"1558-1568","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Fatty acid amides as potential circulating biomarkers for sarcopenia"],"prefix":"10.1002","volume":"14","author":[{"given":"Ye An","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Department of Internal Medicine Veterans Health Service Medical Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung\u2010Min","family":"Koh","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung\u2010hyun","family":"Kwon","sequence":"additional","affiliation":[{"name":"Veterans Medical Research Institute Veterans Health Service Medical Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Veterans Medical Research Institute Veterans Health Service Medical Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han Jin","family":"Cho","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Asan Institute for Life Sciences Asan Medical Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjun","family":"Kim","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Asan Institute for Life Sciences Asan Medical Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su Jung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Department of Internal Medicine Veterans Health Service Medical Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Ju","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3127-7160","authenticated-orcid":false,"given":"Je Hyun","family":"Seo","sequence":"additional","affiliation":[{"name":"Veterans Medical Research Institute Veterans Health Service Medical Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afy169"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2019.110692"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/gln031"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2020.04.002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afq034"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2016.05.016"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2016.162"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12091"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2008.236"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glx139"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/gly100"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12102959"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07567-9"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2019.12.012"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-018-1367-3"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12709"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bimj.200410133"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13048"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/rej.2019.2211"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12144"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/647348"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2014.03.017"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.121079"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114512005740"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1139\/H10-022"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114520004304"},{"key":"e_1_2_7_29_1","article-title":"Alterations of the endocannabinoid system and circulating and peripheral tissue levels of endocannabinoids in sarcopenic rats","author":"Le Bacquer O","year":"2021","journal-title":"J Cachexia Sarcopenia Muscle"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-021-01832-0"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10050552"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.113.176255"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/318981"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080152"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-017-2640-x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00100"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.021915"},{"key":"e_1_2_7_38_1","first-page":"1137","article-title":"Nature, origin and role of fatty acids of the nervous system: an essential fatty acid, an alpha\u2010linolenic acid, changing the structure and the cerebral function","volume":"173","author":"Bourre JM","year":"1989","journal-title":"Bull Acad Natl Med"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr300363b"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b04491"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12899"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13244","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T04:09:40Z","timestamp":1703822980000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.13244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1002\/jcsm.13244"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.13244","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"2022-10-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}