{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:18:18Z","timestamp":1792588698242,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82201726"],"award-info":[{"award-number":["82201726"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Although the adiponectin signalling exerts exercise\u2010mimicking effects, whether this pathway contributes to the anti\u2010ageing benefits of physical exercise has not been established yet.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Swim exercise training and wheel running were used to measure lifespan in the nematode\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                      <\/jats:styled-content>\n                      and skeletal muscle quality in mice, respectively. Muscle weight, muscle fibre cross\u2010sectional area (CSA) and myonuclei number were used to evaluate muscle mass. RNA sequencing (RNA\u2010Seq) analysis of skeletal muscle in exercised mice was used to study the underlying mechanisms. Western blot and immunofluorescence were performed to explore autophagy\u2010 and senescence\u2010related markers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C.\u00a0elegans<\/jats:italic>\n                      <\/jats:styled-content>\n                      adiponectin receptor PAQR\u20101\/AdipoR1, but not PAQR\u20102\/AdipoR2, was activated (3.55\u2010fold and 3.48\u2010fold increases in p\u2010AMPK on Days 1 and 6, respectively,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), which was involved in lifespan extension in exercised worms. Exercise training increased skeletal muscle mass index (1.29\u2010fold,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.01), muscle weight (1.75\u2010fold,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), myonuclei number (1.33\u2010fold,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), muscle fibre CSA (1.39\u2010fold,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and capillary abundance (2.19\u2010fold,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001 for capillary density; 1.58\u2010fold,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.01 for capillary number) in aged mice. Physical exercise reduced protein (2.94\u2010fold,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001) and mRNA levels (1.70\u2010fold,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001) of p16\n                      <jats:sup>INK4a<\/jats:sup>\n                      , a marker for cellular senescence, in skeletal muscle of aged mice. These beneficial effects of exercise on skeletal muscle of mice were dependent on AdipoR1. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis for differentially expressed genes in skeletal muscle between exercised mice with and without AdipoR1 knockdown by RNA\u2010Seq analysis revealed that several KEGG pathways, such as \u2018AMPK signalling pathway\u2019 (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), \u2018FOXO signalling pathway\u2019 (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001) and \u2018autophagy\u2019 (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001) were overrepresented. Knockdown of\n                      <jats:italic>FoxO3a<\/jats:italic>\n                      inhibited exercise\u2010mediated beneficial effects on skeletal muscle quality of mice by inhibiting autophagy\/mitophagy (3.81\u2010fold reduction in LC3\u2010II protein,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001; 1.53\u2010fold reduction in BNIP3 protein,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Knockdown of\n                      <jats:italic>daf\u201016<\/jats:italic>\n                      , the FoxO homologue in\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C.\u00a0elegans<\/jats:italic>\n                      <\/jats:styled-content>\n                      , reduced autophagy (2.77\u2010fold and 2.06\u2010fold reduction in GFP::LGG\u20101 puncta in seam cells and the intestine, respectively,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and blocked lifespan extension by exercise in worms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings provide insights into how the AdipoR1 pathway has an impact on the anti\u2010ageing benefits of exercise and implicate that activation of the AdipoR1 signalling may represent a potential therapeutic strategy for reducing age\u2010related loss of skeletal muscle.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13257","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T03:55:19Z","timestamp":1684900519000},"page":"1789-1801","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Physical exercise attenuates age\u2010related muscle atrophy and exhibits anti\u2010ageing effects via the adiponectin receptor 1 signalling"],"prefix":"10.1002","volume":"14","author":[{"given":"Yuan\u2010Li","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Bio\u2010Resources in Yunnan, School of Life Sciences Yunnan University  Kunming Yunnan China"},{"name":"Faculty of Basic Medicine Kunming Medical University  Kunming Yunnan 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