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CYA had been reported to have ameliorating effects on dexamethasone\u2010induced atrophy of C2C12 mouse skeletal myotubes, but its effects on cancer cachexia were unclear. Here, we checked the influence of CYA on muscle atrophy in cancer cachexia mice and tried to clarify its mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C26 tumour\u2010bearing mice were applied as the animal model to examine the effects of CYA in attenuating cachexia symptoms. The in vitro cell models of TNF\u2010\u03b1\u2010induced C2C12 myotubes or ad\u2010mRFP\u2010GFP\u2010LC3B\u2010transfected C2C12 myotubes were used to check the influence of CYA on myotube atrophy based on both ubiquitin proteasome system (UPS) and autophagy\u2010lysosome system. The possible direct targets of CYA were searched using the biotin\u2010streptavidin pull\u2010down assay and then confirmed using the Microscale thermophoresis binding assay. The levels of related signal proteins in both in vitro and in vivo experiments were examined using western blotting and immunocytochemical assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The administration of CYA prevented body weight loss and muscle wasting in C26 tumour\u2010bearing mice without affecting tumour growth. At the end of the experiment, the body weight of mice treated with 30\u00a0mg\/kg of CYA (23.59\u00a0\u00b1\u00a00.94\u00a0g) was significantly higher than that of the C26 model group (21.66\u00a0\u00b1\u00a00.56\u00a0g) with <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05. The values of gastrocnemius muscle weight\/body weight of mice treated with 15 or 30\u00a0mg\/kg CYA (0.53\u00a0\u00b1\u00a00.02% and 0.54\u00a0\u00b1\u00a00.01%, respectively) were both significantly higher than that of the C26 model group (0.45\u00a0\u00b1\u00a00.01%) with <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01. CYA decreased both UPS\u2010mediated protein degradation and autophagy in muscle tissues of C26 tumour\u2010bearing mice as well as in C2C12 myotubes treated with TNF\u2010\u03b1. The thousand\u2010and\u2010one amino acid kinase 1 (TAOK1) was found to be the direct binding target of CYA. CYA inhibited the activation of TAOK1 and its downstream p38\u2010MAPK pathway thus decreased the level and nuclear location of FoxO3. siRNA knockdown of TAOK1 or regulation of the p38\u2010MAPK pathway using activator or inhibitor could affect the ameliorating effects of CYA on myotube atrophy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CYA ameliorates cancer cachexia muscle atrophy by decreasing both UPS degradation and autophagy. The ameliorating effects of CYA on muscle atrophy might be based on its binding with TAOK1 and inhibiting the TAOK1\/p38\u2010MAPK\/FoxO3 pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13288","type":"journal-article","created":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T08:40:55Z","timestamp":1689237655000},"page":"2098-2113","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Corylifol A ameliorates muscle atrophy by inhibiting TAOK1\/p38\u2010MAPK\/FoxO3 pathway in cancer cachexia"],"prefix":"10.1002","volume":"14","author":[{"given":"Ruiqin","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Frontiers Science Center of TCM Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research Shanghai University of Traditional Chinese Medicine  Shanghai 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