{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T07:46:56Z","timestamp":1792482416781,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T00:00:00Z","timestamp":1689206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82230112"],"award-info":[{"award-number":["82230112"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gut microbiota plays a key role in the development of sarcopenia via the \u2018gut\u2010muscle\u2019 axis, and probiotics\u2010based therapy might be a strategy for sarcopenia. Fecal microbiota transplantation from young donors (yFMT) has attracted much attention because of its probiotic function. However, whether or not yFMT is effective for sarcopenia in old recipients is largely unknown. Thus, we aimed to investigate the effect and mechanism of yFMT on age\u2010related sarcopenia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The fecal microbiota of either young (12\u00a0weeks) or old (88\u00a0weeks) donor rats was transplanted into aged recipient rats for 8\u00a0weeks. Then, muscle mass, muscle strength, muscle function, muscle atrophy, and muscle regeneration capacity were measured. Analysis of fecal 16\u00a0s rRNA, serum non\u2010targeted metabolomic, gut barrier integrity, and muscle transcriptome was conducted to elucidate the interaction between gut microbiota and skeletal muscles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      As evaluated by magnetic resonance imaging examination, grip strength test (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.01), rotarod test (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), and exhaustive running test (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), we found that yFMT mitigated muscle mass loss, muscle strength weakness, and muscle function impairment in aged rats. yFMT also countered age\u2010related atrophy and poor regeneration capacity in fast\u2010 and slow\u2010switch muscles, which were manifested by the decrease in slow\u2010switch myofibres (both\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.01) and muscle interstitial fibrosis (both\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and the increase in the cross\u2010section area of myofibres (both\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), fast\u2010switch myofibres (both\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.01), and muscle satellite cells (both\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001). In addition, yFMT ameliorated age\u2010related dysbiosis of gut microbiota and metabolites by promoting the production of beneficial bacteria and metabolites\u2014\n                      <jats:italic>Akkermansia<\/jats:italic>\n                      ,\n                      <jats:italic>Lactococcus<\/jats:italic>\n                      ,\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      , \u03b3\u2010glutamyltyrosine, 3R\u2010hydroxy\u2010butanoic acid, and methoxyacetic acid and inhibiting the production of deleterious bacteria and metabolites\u2014\n                      <jats:italic>Family_XIII_AD3011_group<\/jats:italic>\n                      ,\n                      <jats:italic>Collinsella<\/jats:italic>\n                      , indoxyl sulfate, indole\u20103\u2010carboxilic acid\u2010\n                      <jats:italic>O<\/jats:italic>\n                      \u2010sulphate, and trimethylamine N\u2010oxide. Also, yFMT prevented age\u2010related destruction of gut barrier integrity by increasing the density of goblet cells (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.0001) and the expression levels of mucin\u20102 (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.0001) and tight junctional proteins (all\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Meanwhile, yFMT attenuated age\u2010related impairment of mitochondrial biogenesis and function in fast\u2010 and slow\u2010switch muscles. Correlation analysis revealed that yFMT\u2010induced alterations of gut microbiota and metabolites might be closely related to mitochondria\u2010related genes and sarcopenia\u2010related phenotypes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>yFMT could reshape the dysbiosis of gut microbiota and metabolites, maintain gut barrier integrity, and improve muscle mitochondrial dysfunction, eventually alleviating sarcopenia in aged rats. yFMT might be a new therapeutic strategy for age\u2010related sarcopenia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13294","type":"journal-article","created":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T04:43:43Z","timestamp":1689223423000},"page":"2168-2183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Faecal microbiota transplantation from young rats attenuates age\u2010related sarcopenia revealed by multiomics analysis"],"prefix":"10.1002","volume":"14","author":[{"given":"Xiaoxing","family":"Mo","sequence":"first","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijie","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunhong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiao","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Health Toxicology, MOE Key Lab of Environment and Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liegang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2022.101720"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2017.60"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan5662"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101262"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12784"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/7026198"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12964"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.17049"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.27014"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-021-00093-9"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18140-1"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12560"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12410"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10070862"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.07.013"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.590869"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12849"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.14876"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2022.04.097"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.864080"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c00245"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-017-0345-5"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.37421"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.02.023"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10101398"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2021.108600"},{"key":"e_1_2_8_28_1","article-title":"TMAO reductase, a biomarker for gut permeability defect induced inflammation, in mouse model of chronic kidney disease and dextran sulfate solution\u2010induced mucositis","author":"Boonhai S","year":"2021","journal-title":"Asian Pac J Allergy Immunol"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP277336"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2021-326789"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2021.1900996"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-322260"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2019.05.007"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3920\/BM2016.0184"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902965R"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13062"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101782"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12202"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-022-02990-5"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12899"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13294","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T21:45:20Z","timestamp":1697147120000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.13294"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,13]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1002\/jcsm.13294"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.13294","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,13]]},"assertion":[{"value":"2022-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}