{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:11:54Z","timestamp":1789204314592,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32100927"],"award-info":[{"award-number":["32100927"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101653"],"award-info":[{"award-number":["82101653"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["CSC 202108510037"],"award-info":[{"award-number":["CSC 202108510037"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,10]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n The gut microbiome and fecal metabolites have been found to influence sarcopenia, but whether there are potential bacteria that can alleviate sarcopenia has been under\u2010investigated, and the molecular mechanism remains unclear.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n To investigate the relationships between the gut microbiome, fecal metabolites and sarcopenia, subjects were selected from observational multi\u2010ethnic study conducted in Western China. Sarcopenia was diagnosed according to the criteria of the Asian Working Group for Sarcopenia 2014. The gut microbiome was profiled by shotgun metagenomic sequencing. Untargeted metabolomic analysis was performed to analyse the differences in fecal metabolites. We investigated bacterium with the greatest relative abundance difference between healthy individuals and sarcopenia patients, and the differences in metabolites associated with the bacteria, to verify its effects on muscle mass and function in a mouse model.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The study included 283 participants (68.90% females, mean age: 66.66\u00a0years old) with and without sarcopenia (141 and 142 participants, respectively) and from the Han (98 participants), Zang (88 participants) and Qiang (97 participants) ethnic groups. This showed an overall reduction (15.03% vs. 20.77%,\n P<\/jats:italic>\n \u00a0=\u00a00.01) of\n \n Prevotella copri<\/jats:italic>\n <\/jats:styled-content>\n between the sarcopenia and non\u2010sarcopenia subjects across the three ethnic groups. Functional characterization of the differential bacteria showed enrichment (odds ratio\u00a0=\u00a015.97,\n P<\/jats:italic>\n \u00a0=\u00a00.0068) in branched chain amino acid (BCAA) metabolism in non\u2010sarcopenia group. A total of 13 BCAA and their derivatives have relatively low levels in sarcopenia. In the in vivo experiment, we found that the blood BCAA level was higher in the mice gavaged with live\n \n P. copri<\/jats:italic>\n <\/jats:styled-content>\n (LPC) (\n P<\/jats:italic>\n \u00a0<\u00a00.001). The LPC mice had significantly longer wire and grid hanging time (\n P<\/jats:italic>\n \u00a0<\u00a00.02), longer time on rotor (\n P<\/jats:italic>\n \u00a0=\u00a00.0001) and larger grip strength (\n P<\/jats:italic>\n \u00a0<\u00a00.0001), indicating better muscle function. The weight of gastrocnemius mass and rectus femoris mass (\n P<\/jats:italic>\n \u00a0<\u00a00.05) was higher in LPC mice. The micro\u2010computed tomography showed a larger leg area (\n P<\/jats:italic>\n \u00a0=\u00a00.0031), and a small animal analyser showed a higher lean mass ratio in LPC mice (\n P<\/jats:italic>\n \u00a0=\u00a00.0157), indicating higher muscle mass.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The results indicated that there were lower levels of both\n \n P. copri<\/jats:italic>\n <\/jats:styled-content>\n and BCAA in sarcopenia individuals. In vivo experiments, gavage with LPC could attenuate muscle mass and function decline, indicating alleviating sarcopenia. This suggested that\n \n P. copri<\/jats:italic>\n <\/jats:styled-content>\n may play a therapeutic potential role in the management of sarcopenia.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcsm.13313","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T14:19:22Z","timestamp":1692627562000},"page":"2275-2288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["\n Prevotella copri<\/i>\n <\/scp>\n alleviates sarcopenia via attenuating muscle mass loss and function decline"],"prefix":"10.1002","volume":"14","author":[{"given":"Xiaolei","family":"Liu","sequence":"first","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"Department of Geriatrics, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiqiu","family":"Wu","sequence":"additional","affiliation":[{"name":"West China Biomedical Big Data Center, West China Hospital\/West China School of Medicine Sichuan University Chengdu China"},{"name":"Department of Genetics, University of Groningen University Medical Center Groningen Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Tang","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"Department of Basic Medicine Chengdu University of Traditional Chinese Medicine Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Xu","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University Luzhou Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bailing","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biotherapy and Cancer Center\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical oncology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center Medicine School of University of Electronic Science and Technology Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjuan","family":"Hu","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"Department of Geriatrics, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gongchang","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"Department of Geriatrics, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Cheng","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"Department of Geriatrics, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Xia","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"Department of Geriatrics, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilong","family":"Chen","sequence":"additional","affiliation":[{"name":"West China Biomedical Big Data Center, West China Hospital\/West China School of Medicine Sichuan University Chengdu China"},{"name":"Med\u2010X Center for Informatics Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Wu","sequence":"additional","affiliation":[{"name":"The College of Life Sciences Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daoming","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Groningen University Medical Center Groningen Groningen The Netherlands"},{"name":"Department of Pediatrics, University of Groningen University Medical Center Groningen Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jirong","family":"Yue","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"Department of Geriatrics, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Dong","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Geriatrics, West China Hospital Sichuan University Chengdu China"},{"name":"State Key Laboratory of Biotherapy, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyuan","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Groningen University Medical Center Groningen Groningen The Netherlands"},{"name":"Department of Pediatrics, University of Groningen University 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afq034"},{"key":"e_1_2_11_3_1","volume-title":"Global prevalence of sarcopenia and severe sarcopenia: a systematic review and meta\u2010analysis","author":"Petermann\u2010Rocha F","year":"2021"},{"key":"e_1_2_11_4_1","first-page":"9","volume-title":"Aging gut microbiota at the cross\u2010road between nutrition, physical frailty, and sarcopenia: is there a gut\u2010muscle axis?","author":"Ticinesi 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