{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T09:26:25Z","timestamp":1782120385784,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T00:00:00Z","timestamp":1695081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["R01 AR065826"],"award-info":[{"award-number":["R01 AR065826"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["T32 HL076122"],"award-info":[{"award-number":["T32 HL076122"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cancer and its treatment can adversely affect skeletal muscle, impacting physical function, treatment response and survival. No studies, however, have comprehensively characterized these muscle adaptations longitudinally in human patients at the cellular level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined skeletal muscle size and function from the whole body to the sub\u2010cellular level in 11 patients with non\u2010small cell lung cancer (NSCLC; 6 male\/5 female, mean age 58\u00a0\u00b1\u00a03\u00a0years) studied over a 2\u2010month observation period starting during their first cycle of standard of care cancer treatment and in 11 age\u2010 and sex\u2010matched healthy controls (HC) without a current or past history of cancer. Biopsies of the vastus lateralis were performed to assess muscle fibre size, contractility and mitochondrial content, along with assessments of physical function, whole muscle size and function, and circulating cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Body weight, composition and thigh muscle area and density were unaltered over time in patients with NSCLC, while muscle density was lower in patients with NSCLC versus HC (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.03). Skeletal muscle fibre size decreased by 18% over time in patients (all <jats:italic>P<\/jats:italic>\u00a0=\u00a00.02) and was lower than HC (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.02). Mitochondrial fractional area and density did not change over time in patients, but fractional area was lower in patients with NSCLC compared with HC (subsarcolemmal, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.04; intermyofibrillar, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.03). Patients with NSCLC had higher plasma concentrations of IL\u20106 (HC 1.40\u00a0\u00b1\u00a00.50; NSCLC 4.71\u00a0\u00b1\u00a04.22; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01), GDF\u201015 (HC 569\u00a0\u00b1\u00a0166; NSCLC 2071\u00a0\u00b1\u00a01168; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) and IL\u20108\/CXCL8 (HC 4.9\u00a0\u00b1\u00a01.8; NSCLC 10.1\u00a0\u00b1\u00a06.0; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.02) compared with HC, but there were no changes in inflammatory markers in patients with NSCLC over time. No changes were observed in markers of satellite cell activation or DNA damage in patients and no group differences were noted with HC. Whole\u2010muscle strength was preserved over time in patients with NSCLC coincident with improved single fibre contractility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study is the first to comprehensively examine longitudinal alterations in skeletal muscle fibre size and function in patients with NSCLC and suggests that muscle fibre atrophy occurs during cancer treatment despite weight stability and no changes in conventional clinical measurements of whole body or thigh muscle size over this period.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13332","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T06:10:29Z","timestamp":1695190229000},"page":"2579-2590","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Skeletal muscle adaptations in patients with lung cancer: Longitudinal observations from the whole body to cellular level"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2189-9441","authenticated-orcid":false,"given":"Deena B.","family":"Snoke","sequence":"first","affiliation":[{"name":"Department of Medicine University of Vermont Larner College of Medicine  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Bellefleur","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Vermont Larner College of Medicine  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hibba Tul","family":"Rehman","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Vermont Larner College of Medicine  Burlington Vermont USA"},{"name":"University of Vermont Cancer Center University of Vermont Larner College of Medicine  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Carson","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy The University of Tennessee Health Science Center  Memphis Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew E.","family":"Poynter","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Vermont Larner College of Medicine  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim L.","family":"Dittus","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Vermont Larner College of Medicine  Burlington Vermont USA"},{"name":"University of Vermont Cancer Center University of Vermont Larner College of Medicine  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0668-1911","authenticated-orcid":false,"given":"Michael J.","family":"Toth","sequence":"additional","affiliation":[{"name":"Department 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