{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:18:36Z","timestamp":1791591516415,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:00:00Z","timestamp":1695513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015918","name":"Dipartimento di Scienze Mediche, Universit\u00e0 degli Studi di Torino","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015918","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004271","name":"Sapienza Universit\u00e0 di Roma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004271","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Small non\u2010coding (snc)RNAs, including microRNAs and P\u2010element induced wimpy testis (PIWI)\u2010interacting\u2010RNAs (piRNAs), crucially regulate gene expression in both physiological and pathological conditions. In particular, some muscle\u2010specific microRNAs (myomiRs) have been involved in the pathogenesis of cancer\u2010induced muscle wasting. The aims of the present study were (i) to profile sncRNAs in both skeletal muscle and plasma of gastrointestinal cancer patients and (ii) to investigate the association among differentially expressed sncRNAs and the level of muscularity at body composition analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Surgical patients with gastrointestinal cancer or benign disease were recruited. Blood samples and muscle biopsies (\n                      <jats:italic>rectus abdominis<\/jats:italic>\n                      ) were collected during surgery. Low muscularity patients were those at the lowest tertile of skeletal muscle index (SMI; CT\u2010scan), whereas moderate\/high muscularity patients were in the middle and highest SMI tertiles. SncRNAs in the muscle were assessed by RNAseq, circulating microRNAs were evaluated by qPCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Cancer patients (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a025; 13 females, 52%) showed a mean age of 71.6\u00a0\u00b1\u00a011.2\u00a0years, a median body weight loss of 4.2% and a mean BMI of 27.0\u00a0\u00b1\u00a03.2\u00a0kg\/m\n                      <jats:sup>2<\/jats:sup>\n                      . Control group (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a015; 9 females, 60%) showed a mean age 58.1\u00a0\u00b1\u00a013.9\u00a0years and a mean BMI of 28.0\u00a0\u00b1\u00a04.3\u00a0kg\/m\n                      <jats:sup>2<\/jats:sup>\n                      . In cancer patients, the median L3\u2010SMI (cm\n                      <jats:sup>2<\/jats:sup>\n                      \/m\n                      <jats:sup>2<\/jats:sup>\n                      ) was 42.52 (34.42; 49.07). Males showed a median L3\u2010SMI of 46.08 (41.17\u201351.79) and females a median L3\u2010SMI of 40.77 (33.73\u201342.87). Moderate\u2010high and low muscularity groups included 17 and 8 patients, respectively. As for circulating microRNAs, miR\u201021\u20105p and miR\u2010133a\u20103p were up\u2010regulated in patients compared with controls, whereas miR\u201015b\u20105p resulted down\u2010regulated in the same comparison (about 30% of control values). Sample clustering by muscularity and sex revealed increased miR\u2010133a\u20103p and miR\u2010206 only in moderate\u2010high muscularity males. SncRNA profiling in the muscle identified 373 microRNAs and 190 piRNAs (72.5% and 18.7% of raw reads, respectively). As for microRNAs, 10 were up\u2010regulated, and 56 were down\u2010regulated in cancer patients versus controls. Among the 24 dysregulated piRNAs, the majority were down\u2010regulated, including the top two most expressed piRNAs in the muscle (piR\u201012790 and piR\u20102106). Network analysis on validated mRNA targets of down\u2010regulated microRNAs revealed miR\u201015b\u20105p, miR\u2010106a\u20105p and miR\u2010106b\u20105p as main interactors of genes related to ubiquitin ligase\/transferase activities.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results show dysregulation of both muscle microRNAs and piRNAs in cancer patients compared with controls, the former following a sex\u2010specific pattern. Changes in circulating microRNAs are associated with the degree of muscularity rather than body weight loss.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13343","type":"journal-article","created":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T22:04:37Z","timestamp":1695593077000},"page":"2692-2702","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Small non\u2010coding RNA profiling in patients with gastrointestinal cancer"],"prefix":"10.1002","volume":"14","author":[{"given":"Alessio","family":"Molfino","sequence":"first","affiliation":[{"name":"Department of Translational and Precision Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6070-9861","authenticated-orcid":false,"given":"Marc","family":"Beltr\u00e0","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Torino  Torino Italy"},{"name":"Institute for Research in Biomedicine (IRB Barcelona) The Barcelona Institute of Science and Technology  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ida","family":"Amabile","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Belli","sequence":"additional","affiliation":[{"name":"Department of Translational and Precision Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Birolo","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Belloni","sequence":"additional","affiliation":[{"name":"Department of Medical\u2010Surgical Sciences and Translational Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"De Lucia","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Garcia\u2010Castillo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Penna","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Imbimbo","sequence":"additional","affiliation":[{"name":"Department of Translational and Precision Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Nigri","sequence":"additional","affiliation":[{"name":"Department of Medical\u2010Surgical Sciences and Translational Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Pardini","sequence":"additional","affiliation":[{"name":"Italian Institute for Genomic Medicine (IIGM)  Candiolo Italy"},{"name":"Candiolo Cancer Institute FPO\u2010IRCCS  Candiolo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1216-7230","authenticated-orcid":false,"given":"Paola","family":"Costelli","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Muscaritoli","sequence":"additional","affiliation":[{"name":"Department of Translational and Precision Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21239268"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20123-1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12071864"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.105"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13073"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/801685"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4467"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3838"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000645"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22169007"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2210"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902468RR"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12227"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12512"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70218-7"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12168"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2020.05.023"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.775369"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12844"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1402714111"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2021.09.007"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00006.2017"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.11.002"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.02.009"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes12020176"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.11957"},{"key":"e_1_2_8_28_1","first-page":"1555","article-title":"Prognostic impact of sarcopenia and its correlation with circulating miR\u201021 in colorectal cancer patients","volume":"39","author":"Okugawa Y","year":"2018","journal-title":"Oncol Rep"},{"key":"e_1_2_8_29_1","first-page":"124","article-title":"The role of microRNA in cancer cachexia and muscle wasting: a review article","volume":"12","author":"Sutandyo N","year":"2021","journal-title":"Caspian J Intern Med"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.194175"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12877-021-02040-0"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/rco2.23"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00054.2017"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089065"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-00014-1"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/2042-6410-5-3"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-020-00628-w"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00251.2005"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23139-2"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI156125"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00251.2005"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13343","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:07:41Z","timestamp":1704931661000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.13343"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,24]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/jcsm.13343"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.13343","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,24]]},"assertion":[{"value":"2022-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}