{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T03:57:33Z","timestamp":1789617453404,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sepsis\u2010associated muscle weakness is common in patients of intensive care units (ICUs), and it is closely associated with poor outcomes. The mechanism of sepsis\u2010induced muscle weakness is unclear. Recent studies have found that gut microbiota and metabolites are involved in the regulation of skeletal muscle mass and metabolism. This study aimed to investigate the effects of gut microbiota and metabolites on sepsis\u2010associated muscle weakness.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In a lipopolysaccharide (LPS)\u2010induced inflammation mouse model, mice with different sensitivities to LPS\u2010induced inflammation were considered as donor mice for the faecal microbiota transplantation (FMT) assay, and recipient mice were divided into sensitive (Sen) and resistant (Res) groups. Skeletal muscle mass and function, as well as colonic barrier integrity were tested and gut microbiota and metabolite composition were analysed in both groups of mice. The effect of intestinal differential metabolite vitamin K1 on LPS\u2010triggered muscle damage was investigated, and the underlying mechanism was explored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Recipients exhibited varying LPS\u2010triggered muscle damage and intestinal barrier disruption. Tibialis anterior (TA) muscle of Sen exhibited upregulated expression levels of MuRF\u20101 (0.825\u00a0\u00b1\u00a00.063 vs. 0.304\u00a0\u00b1\u00a00.293,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0141) and MAFbx (1.055\u00a0\u00b1\u00a00.079 vs. 0.456\u00a0\u00b1\u00a00.3,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0092). Colonic tight junction proteins ZO\u20101 (0.550\u00a0\u00b1\u00a00.087 vs. 0.842\u00a0\u00b1\u00a00.094,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0492) and occludin (0.284\u00a0\u00b1\u00a00.057 vs. 0.664\u00a0\u00b1\u00a00.191,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0487) were significantly downregulated in the Sen group. Metabolomic analysis showed significantly higher vitamin K1 in the faeces (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0195) and serum of the Res group (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0079) than those of the Sen group. After vitamin K1 intervention, muscle atrophy\u2010related protein expression downregulated (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Meanwhile SIRT1 protein expression were upregulated (0.320\u00a0\u00b1\u00a00.035 vs. 0.685\u00a0\u00b1\u00a00.081,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0281) and pNF\u2010\u03baB protein expression were downregulated (0.815\u00a0\u00b1\u00a00.295 vs. 0.258\u00a0\u00b1\u00a00.130,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0308). PI3K (0.365\u00a0\u00b1\u00a00.142 vs. 0.763\u00a0\u00b1\u00a00.013,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0475), pAKT (0.493\u00a0\u00b1\u00a00.159 vs. 1.183\u00a0\u00b1\u00a00.344,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0254) and pmTOR (0.509\u00a0\u00b1\u00a00.088 vs. 1.110\u00a0\u00b1\u00a00.190,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0368) protein expression levels were upregulated in TA muscle. Meanwhile, vitamin K1 attenuated serum inflammatory factor levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Vitamin K1 might ameliorate LPS\u2010triggered skeletal muscle damage by antagonizing NF\u2010\u03baB\u2010mediated inflammation through upregulation of SIRT1 and regulating the balance between protein synthesis and catabolism.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13379","type":"journal-article","created":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T02:58:39Z","timestamp":1701226719000},"page":"81-97","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Vitamin K1 ameliorates lipopolysaccharide\u2010triggered skeletal muscle damage revealed by faecal bacteria transplantation"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6501-1331","authenticated-orcid":false,"given":"Yuru","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The Affiliated Hospital of Southwest Medical University  Luzhou China"},{"name":"Anesthesiology and Critical Care Medicine Key Laboratory of Luzhou Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Affiliated Hospital of Southwest Medical University  Luzhou China"},{"name":"Anesthesiology and Critical Care Medicine Key Laboratory of Luzhou Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Affiliated Hospital of Southwest Medical University  Luzhou China"},{"name":"Anesthesiology and Critical Care Medicine Key Laboratory of Luzhou Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Affiliated Hospital of Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingshun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen and Microbiology Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangyong","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fasheng","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuexin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0680-5139","authenticated-orcid":false,"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Affiliated Hospital of Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201411-2011ST"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2018.12.45"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000053651.38421.D9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2010.1553"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-014-3224-9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2013.278481"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30361"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-84031-0"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2021.1900593"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12010065"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan5662"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31786"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15693"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222212435"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18848"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.02.024"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.736204"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09922"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.618249"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.704044"},{"key":"e_1_2_9_22_1","doi-asserted-by":"crossref","unstructured":"In vivo electrophysiological measurement of the rat ulnar nerve with axonal excitability testing. United States;2018.https:\/\/doi.org\/10.3791\/56102","DOI":"10.3791\/56102"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00261.2018"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-021-00597-0"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2045876"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2021.1966263"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-321747"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-0711-6"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-00545-z"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-022-04416-w"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10071571"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.70.926"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00796.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.45.2.223"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1086\/315093"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-57714-3"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-113-3-227"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2015.12.031"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00204.2014"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13379","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T21:29:08Z","timestamp":1706822948000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.13379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,28]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/jcsm.13379"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.13379","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,28]]},"assertion":[{"value":"2023-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}