{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T01:23:37Z","timestamp":1773883417372,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Skeletal muscle mass wasting almost invariably accompanies bone loss in elderly, and the coexistence of these two conditions depends on the tight endocrine crosstalk existing between the two organs, other than the biomechanical coupling. Since the current diagnostics limitation in this field, and given the progressive population aging, more effective tools are needed. The aim of this study was to identify circulating microRNAs (miRNAs) as potential biomarkers for muscle mass wasting in post\u2010menopausal osteoporotic women.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred seventy\u2010nine miRNAs were assayed by quantitative real\u2010time polymerase chain reaction in plasma samples from 28 otherwise healthy post\u2010menopausal osteoporotic women (73.4\u00a0\u00b1\u00a06.6\u00a0years old). The cohort was divided in tertiles based on appendicular skeletal muscle mass index (ASMMI) to better highlight the differences on skeletal muscle mass (first tertile: <jats:italic>n<\/jats:italic>\u00a0=\u00a09, ASMMI\u00a0=\u00a04.88\u00a0\u00b1\u00a00.40\u00a0kg\u00b7m<jats:sup>\u22122<\/jats:sup>; second tertile: <jats:italic>n<\/jats:italic>\u00a0=\u00a010, ASMMI\u00a0=\u00a05.73\u00a0\u00b1\u00a00.23\u00a0kg\u00b7m<jats:sup>\u22122<\/jats:sup>; third tertile: <jats:italic>n<\/jats:italic>\u00a0=\u00a09, ASMMI\u00a0=\u00a06.40\u00a0\u00b1\u00a00.22\u00a0kg\u00b7m<jats:sup>\u22122<\/jats:sup>). Receiver operating characteristic (ROC) curves were calculated to estimate the diagnostic potential of miRNAs. miRNAs displaying a statistically significant fold change\u00a0\u2265\u00a0\u00b11.5 and area under the curve (AUC)\u00a0&gt;\u00a00.800 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) between the first and third tertiles were considered. A linear regression model was applied to estimate the association between miRNA expression and ASMMI in the whole population, adjusting for body mass index, age, total fat (measured by total\u2010body dual\u2010energy X\u2010ray absorptiometry [DXA]) and bone mineral density (measured by femur DXA). Circulating levels of adipo\u2010myokines were evaluated by bead\u2010based immunofluorescent assays and enzyme\u2010linked immunosorbent assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five miRNAs (hsa\u2010miR\u2010221\u20103p, hsa\u2010miR\u2010374b\u20105p, hsa\u2010miR\u2010146a\u20105p, hsa\u2010miR\u2010126\u20105p and hsa\u2010miR\u2010425\u20105p) resulted down\u2010regulated and two miRNAs (hsa\u2010miR\u2010145\u20105p and hsa\u2010miR\u201025\u20103p) were up\u2010regulated in the first tertile (relative\u2010low ASMMI) compared with the third tertile (relative\u2010high ASMMI) (fold change\u00a0\u2265\u00a0\u00b11.5; <jats:italic>P<\/jats:italic>\u2010value\u00a0&lt;\u00a00.05). All the corresponding ROC curves had AUC\u00a0&gt;\u00a00.8 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Two signatures hsa\u2010miR\u2010126\u20105p, hsa\u2010miR\u2010146a\u20105p and hsa\u2010miR\u2010425\u20105p; and hsa\u2010miR\u2010126\u20105p, hsa\u2010miR\u2010146a\u20105p, hsa\u2010miR\u2010145\u20105p and hsa\u2010miR\u201025\u20103p showed the highest AUC, 0.914 (sensitivity\u00a0=\u00a077.78%; specificity\u00a0=\u00a0100.00%) and 0.901 (sensitivity\u00a0=\u00a088.89%; specificity\u00a0=\u00a0100.00%), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In this study, we identified, for the first time, two miRNA signatures, hsa\u2010miR\u2010126\u20105p, hsa\u2010miR\u2010146a\u20105p and hsa\u2010miR\u2010425\u20105p; and hsa\u2010miR\u2010126\u20105p, hsa\u2010miR\u2010146a\u20105p, hsa\u2010miR\u2010145\u20105p and hsa\u2010miR\u201025\u20103p, specifically associated with muscle mass wasting in post\u2010menopausal osteoporotic women.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13421","type":"journal-article","created":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T03:49:25Z","timestamp":1706240965000},"page":"690-701","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Plasma microRNA signature associated with skeletal muscle wasting in post\u2010menopausal osteoporotic women"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2047-7255","authenticated-orcid":false,"given":"Martina","family":"Faraldi","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Biochemistry and Molecular Biology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0337-975X","authenticated-orcid":false,"given":"Veronica","family":"Sansoni","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Biochemistry and Molecular Biology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacopo","family":"Vitale","sequence":"additional","affiliation":[{"name":"Laboratory of Movement and Sport Science IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Perego","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Biochemistry and Molecular Biology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4379-7374","authenticated-orcid":false,"given":"Marta","family":"Gomarasca","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Biochemistry and Molecular Biology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6644-5163","authenticated-orcid":false,"given":"Chiara","family":"Verdelli","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Endocrinology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7518-5773","authenticated-orcid":false,"given":"Carmelo","family":"Messina","sequence":"additional","affiliation":[{"name":"OU Diagnostic and Interventional Radiology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"},{"name":"Department of Biomedical Science for Health University of Milan  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0759-8431","authenticated-orcid":false,"given":"Luca M.","family":"Sconfienza","sequence":"additional","affiliation":[{"name":"OU Diagnostic and Interventional Radiology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"},{"name":"Department of Biomedical Science for Health University of Milan  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9578-5338","authenticated-orcid":false,"given":"Giuseppe","family":"Banfi","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Biochemistry and Molecular Biology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8140-3175","authenticated-orcid":false,"given":"Sabrina","family":"Corbetta","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Surgical and Dental Sciences University of Milan  Milan Italy"},{"name":"Endocrinology and Diabetology Service IRCCS Istituto Ortopedico Galeazzi  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8365-985X","authenticated-orcid":false,"given":"Giovanni","family":"Lombardi","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Biochemistry and Molecular Biology IRCCS Istituto Ortopedico Galeazzi  Milan Italy"},{"name":"Department of Athletics, Strength and Conditioning Pozna\u0144 University of Physical Education  Pozna\u0144 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.6.785"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2011.01.003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-017-4151-8"},{"key":"e_1_2_9_5_1","first-page":"1","article-title":"Assessment of fracture risk and its application to screening for postmenopausal osteoporosis. Report of a WHO Study Group","volume":"843","year":"1994","journal-title":"World Health Organ Tech Rep Ser"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2017.11.007"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/13697137.2015.1092342"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12048"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12877-021-02608-w"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2018.07.007"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3838"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.911.BJR-2020-0178.R1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020276"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10040589"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00167"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/1759720X20953331"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2020.115350"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-020-00802-3"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.669704"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afz046"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2020.010703"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-38505-x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0206239"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0258(19980430)17:8<857::AID-SIM777>3.0.CO;2-E"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/gly207"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12877-021-02040-0"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/2044-5040-1-19"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.4262"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1515-4"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24495"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11040755"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-254490"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13098-022-00942-6"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.113.280883"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100677"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmx052"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5276271"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.08.079"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00042.2009"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP283209"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00148.2010"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.10.086"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2017.03.027"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-02989-x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acc.2019.07.010"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T08:34:15Z","timestamp":1718699655000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.13421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,25]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/jcsm.13421"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.13421","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,25]]},"assertion":[{"value":"2023-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}