{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:28Z","timestamp":1786977928395,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["201706320322"],"award-info":[{"award-number":["201706320322"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["73111208\u2010SFB 829"],"award-info":[{"award-number":["73111208\u2010SFB 829"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021821","name":"Oncode Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021821","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2024,6]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Sarcopenia is characterized by loss of skeletal muscle mass and function, and is a major risk factor for disability and independence in the elderly. Effective medication is not available. Dietary restriction (DR) has been found to attenuate aging and aging\u2010related diseases, including sarcopenia, but the mechanism of both DR and sarcopenia are incompletely understood.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In this study, mice body weight, fore and all limb grip strength, and motor learning and coordination performance were first analysed to evaluate the DR effects on muscle functioning. Liquid chromatography\u2013mass spectrometry (LC\u2013MS) was utilized for the metabolomics study of the DR effects on sarcopenia in progeroid DNA repair\u2010deficient\n Ercc1<\/jats:italic>\n \n \u2206\/<\/jats:italic>\n \u2212\n <\/jats:sup>\n and\n Xpg<\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice, to identify potential biomarkers for attenuation of sarcopenia.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Muscle mass was significantly (\n P<\/jats:italic>\n \u00a0<\u00a00.05) decreased (13\u201320%) by DR; however, the muscle quality was improved with retained fore limbs and all limbs grip strength in\n Ercc1<\/jats:italic>\n \n \u2206\/<\/jats:italic>\n \u2212\n <\/jats:sup>\n and\n Xpg<\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice. The LC\u2013MS results revealed that metabolites and pathways related to oxidative\u2010stress, that is, GSSG\/GSH (\n P<\/jats:italic>\n \u00a0<\u00a00.01); inflammation, that is, 9\u2010HODE, 11\u2010HETE (\n P<\/jats:italic>\n \u00a0<\u00a00.05), PGE\n 2<\/jats:sub>\n , PGD\n 2<\/jats:sub>\n , and TXB\n 2<\/jats:sub>\n (\n P<\/jats:italic>\n \u00a0<\u00a00.01); and muscle growth (PGF\n 2\u03b1<\/jats:sub>\n ) (\n P<\/jats:italic>\n \u00a0<\u00a00.01) and regeneration stimulation (PGE\n 2<\/jats:sub>\n ) (\n P<\/jats:italic>\n \u00a0<\u00a00.05) are significantly downregulated by DR. On the other hand, anti\u2010inflammatory indicator and several related metabolites, that is, \u03b2\u2010hydroxybutyrate (\n P<\/jats:italic>\n \u00a0<\u00a00.01), 14,15\u2010DiHETE (\n P<\/jats:italic>\n \u00a0<\u00a00.0001), 8,9\u2010EET, 12,13\u2010DiHODE, and PGF\n 1<\/jats:sub>\n (\n P<\/jats:italic>\n \u00a0<\u00a00.05); consumption of sources of energy (i.e., muscle and liver glycogen); and energy production pathways, that is, glycolysis (glucose, glucose\u20106\u2010P, fructose\u20106\u2010P) (\n P<\/jats:italic>\n \u00a0<\u00a00.01), tricarboxylic acid cycle (succinyl\u2010CoA, malate) (\n P<\/jats:italic>\n \u00a0<\u00a00.001), and gluconeogenesis\u2010related metabolite, alanine (\n P<\/jats:italic>\n \u00a0<\u00a00.01), are significantly upregulated by DR. The notably (\n P<\/jats:italic>\n \u00a0<\u00a00.01) down\u2010modulated muscle growth (PGF\n 2\u03b1<\/jats:sub>\n ) and regeneration (PGE\n 2<\/jats:sub>\n ) stimulation metabolite and the increased consumption of glycogen in muscle and liver may be related to the significantly (\n P<\/jats:italic>\n \u00a0<\u00a00.01) lower body weight and muscle mass by DR. The downregulated oxidative stress, pro\u2010inflammatory mediators, and upregulated anti\u2010inflammatory metabolites resulted in a lower energy expenditure, which contributed to enhanced muscle quality together with upregulated energy production pathways by DR. The improved muscle quality may explain why grip strength is maintained and motor coordination and learning performance are improved by DR in\n Ercc1<\/jats:italic>\n \n \u2206\/\u2212<\/jats:italic>\n <\/jats:sup>\n and\n Xpg<\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n This study provides fundamental supporting information on biomarkers and pathways related to the attenuation of sarcopenia, which might facilitate its diagnosis, prevention, and clinical therapy.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcsm.13433","type":"journal-article","created":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T01:45:47Z","timestamp":1714527947000},"page":"868-882","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Metabolomic analysis of dietary\u2010restriction\u2010induced attenuation of sarcopenia in prematurely aging DNA repair\u2010deficient mice"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3453-9499","authenticated-orcid":false,"given":"Yupeng","family":"He","sequence":"first","affiliation":[{"name":"Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research Leiden University Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research Leiden University Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luojiao","family":"Huang","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research Leiden University Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlien Admiraal\u2010van","family":"Mever","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research Leiden University Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rawi","family":"Ramautar","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research Leiden University Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Harms","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research Leiden University Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Rijksen","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology Utrecht The Netherlands"},{"name":"Oncode Institute Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata M.C.","family":"Brandt","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Erasmus MC Cancer Institute Erasmus University Medical Center Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sander","family":"Barnhoorn","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Erasmus MC Cancer Institute Erasmus University Medical Center Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly","family":"Smit","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology Utrecht The Netherlands"},{"name":"Oncode Institute Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dick","family":"Jaarsma","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Erasmus University Medical Center Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Lindenburg","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research Leiden University Leiden The Netherlands"},{"name":"Research Group Metabolomics, Leiden Center for Applied Bioscience University of Applied Sciences Leiden Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan H. 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