{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T02:02:16Z","timestamp":1785463336527,"version":"3.56.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T00:00:00Z","timestamp":1721001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["400362"],"award-info":[{"award-number":["400362"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Mitochondria represent key organelles influencing cellular homeostasis and have been implicated in the signalling events regulating protein synthesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined whether mitochondrial bioenergetics (oxidative phosphorylation and reactive oxygen species (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) emission, ROS) measured <jats:italic>in vitro<\/jats:italic> in permeabilized muscle fibres represent regulatory factors for integrated daily muscle protein synthesis rates and skeletal muscle mass changes across the spectrum of physical activity, including free\u2010living and bed\u2010rest conditions: <jats:italic>n<\/jats:italic>\u00a0=\u00a019 healthy, young men (26\u00a0\u00b1\u00a04\u00a0years, 23.4\u00a0\u00b1\u00a03.3\u00a0kg\/m<jats:sup>2<\/jats:sup>) and following 12\u00a0weeks of resistance\u2010type exercise training: <jats:italic>n<\/jats:italic>\u00a0=\u00a010 healthy older men (70\u00a0\u00b1\u00a03\u00a0years, 25.2\u00a0\u00b1\u00a02.1\u00a0kg\/m<jats:sup>2<\/jats:sup>). Additionally, we evaluated the direct relationship between attenuated mitochondrial ROS emission and integrated daily myofibrillar and sarcoplasmic protein synthesis rates in genetically modified mice (mitochondrial\u2010targeted catalase, MCAT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Neither oxidative phosphorylation nor H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> emission were associated with muscle protein synthesis rates in healthy young men under free\u2010living conditions or following 1\u00a0week of bed rest (both <jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05). Greater increases in GSSG concentration were associated with greater skeletal muscle mass loss following bed rest (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.49, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). In older men, only submaximal mitochondrial oxidative phosphorylation (corrected for mitochondrial content) was positively associated with myofibrillar protein synthesis rates during exercise training (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.72, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). However, changes in oxidative phosphorylation and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> emission were not associated with changes in skeletal muscle mass following training (both <jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05). Additionally, MCAT mice displayed no differences in myofibrillar (2.62\u00a0\u00b1\u00a00.22 vs. 2.75\u00a0\u00b1\u00a00.15%\/day) and sarcoplasmic (3.68\u00a0\u00b1\u00a00.35 vs. 3.54\u00a0\u00b1\u00a00.35%\/day) protein synthesis rates when compared with wild\u2010type mice (both <jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Mitochondrial oxidative phosphorylation and reactive oxygen emission do not seem to represent key factors regulating muscle protein synthesis or muscle mass regulation across the spectrum of physical activity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13532","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T11:00:51Z","timestamp":1721041251000},"page":"1811-1822","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Mitochondrial bioenergetics are not associated with myofibrillar 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