{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T05:48:06Z","timestamp":1792129686683,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005006","name":"Asan Institute for Life Sciences, Asan Medical Center","doi-asserted-by":"publisher","award":["2023IP0041"],"award-info":[{"award-number":["2023IP0041"]}],"id":[{"id":"10.13039\/501100005006","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["2022R1C1C1002929"],"award-info":[{"award-number":["2022R1C1C1002929"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["2022R1A2C1007901"],"award-info":[{"award-number":["2022R1A2C1007901"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["2022R1A2C1003661"],"award-info":[{"award-number":["2022R1A2C1003661"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sarcopenia is an age\u2010related progressive loss of muscle mass and function. Sarcopenia is a multifactorial disorder, including metabolic disturbance; therefore, metabolites may be used as circulating biomarkers for sarcopenia. We aimed to investigate potential biomarkers of sarcopenia using metabolomics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After non\u2010targeted metabolome profiling of plasma from mice of an aging mouse model of sarcopenia, sphingolipid metabolites and muscle cells from the animal model were evaluated using targeted metabolome profiling. The associations between sphingolipid metabolites identified from mouse and cell studies and sarcopenia status were assessed in men in an age\u2010matched discovery (72 cases and 72 controls) and validation (36 cases and 128 controls) cohort; women with sarcopenia (36 cases and 36 controls) were also included as a discovery cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both non\u2010targeted and targeted metabolome profiling in the experimental studies showed an association between sphingolipid metabolites, including ceramides (CERs) and sphingomyelins (SMs), and sarcopenia. Plasma SM (16:0), CER (24:1), and SM (24:1) levels in men with sarcopenia were significantly higher in the discovery cohort than in the controls (all <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). There were no significant differences in plasma sphingolipid levels for women with or without sarcopenia. In men in the discovery cohort, an area under the receiver\u2010operating characteristic curve (AUROC) of SM (16:0) for low muscle strength and low muscle mass was 0.600 (95% confidence interval [CI]: 0.501\u20130.699) and 0.647 (95% CI: 0.557\u20130.737). The AUROC (95% CI) of CER (24:1) and SM (24:1) for low muscle mass in men was 0.669 (95% CI: 0.581\u20130.757) and 0.670 (95% CI: 0.582\u20130.759), respectively. Using a regression equation combining CER (24:1) and SM (16:0) levels, a sphingolipid (SphL) score was calculated; an AUROC of the SphL score for sarcopenia was 0.712 (95% CI: 0.626\u20130.798). The addition of the SphL score to HGS significantly improved the AUC from 0.646 (95% CI: 0.575\u20130.717; HGS only) to 0.751 (95% CI: 0.671\u20130.831, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.002; HGS\u00a0+\u00a0SphL) in the discovery cohort. The predictive ability of the SphL score for sarcopenia was confirmed in the validation cohort (AUROC\u00a0=\u00a00.695, 95% CI: 0.591\u20130.799).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SM (16:0), reflecting low muscle strength, and CER (24:1) and SM (16:0), reflecting low muscle mass, are potential circulating biomarkers for sarcopenia in men. Further research on sphingolipid metabolites is required to confirm these results and provide additional insights into the metabolomic changes relevant to the pathogenesis and diagnosis of sarcopenia.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcsm.13582","type":"journal-article","created":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T11:14:35Z","timestamp":1725448475000},"page":"2476-2486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Sphingolipid metabolites as potential circulating biomarkers for sarcopenia in men"],"prefix":"10.1002","volume":"15","author":[{"given":"Je Hyun","family":"Seo","sequence":"first","affiliation":[{"name":"Veterans Health Service Medical Center Veterans Medical Research Institute  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung\u2010Min","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division 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Medicine, Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pil Whan","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Seoul Now Hospital  Anyang South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Jin","family":"Bae","sequence":"additional","affiliation":[{"name":"Health Screening and Promotion Center, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Kyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Health Screening and Promotion Center, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Ju","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0496-247X","authenticated-orcid":false,"given":"Seung Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Endocrinology and Metabolism, Asan Medical Center University of Ulsan College of Medicine  Seoul South 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