{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T01:44:31Z","timestamp":1793411071679,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T00:00:00Z","timestamp":1737763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T00:00:00Z","timestamp":1737763200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003653","name":"Korea National Institute of Health","doi-asserted-by":"publisher","award":["R01AG031743"],"award-info":[{"award-number":["R01AG031743"]}],"id":[{"id":"10.13039\/501100003653","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>With a decline of 17\u03b2\u2010estradiol (E2) at menopause, E2 has been implicated in the accompanied loss of skeletal muscle mass and strength. We aimed at characterizing transcriptomic responses of skeletal muscle to E2 in female mice, testing the hypothesis that genes and pathways related to contraction and maintenance of mass are differentially expressed in ovariectomized mice with and without E2 treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Soleus and tibialis anterior (TA) muscles from C57BL\/6 ovariectomized mice treated with placebo (OVX) or E2 (OVX\u2009+\u2009E2) for 60\u2009days, or from skeletal muscle\u2010specific ER\u03b1 knockout (skmER\u03b1KO) mice and wild\u2010type littermates (skmER\u03b1WT), were used for genome\u2010wide expression profiling, quantitative real\u2010time PCR and immunoblotting. Computational detection of estrogen response elements (EREs) was performed with EREFINDER.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found 155 significantly regulated probe sets in response to E2 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.001). Pathway analyses identified proteasome and ubiquitin\u2010mediated proteolysis as two downregulated pathways in the E2 group. We confirmed downregulation (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.05) in levels of\n                      <jats:italic>Fbxw7<\/jats:italic>\n                      ,\n                      <jats:italic>Psmb6<\/jats:italic>\n                      ,\n                      <jats:italic>Ube2h<\/jats:italic>\n                      and\n                      <jats:italic>Ubxn1<\/jats:italic>\n                      , as well as pro\u2010apoptotic\n                      <jats:italic>Bnip3<\/jats:italic>\n                      and inflammatory factor\n                      <jats:italic>Nfkbia<\/jats:italic>\n                      . Computational analysis identified ERE in the promoter regions of\n                      <jats:italic>Psmb6<\/jats:italic>\n                      ,\n                      <jats:italic>Ube2h<\/jats:italic>\n                      ,\n                      <jats:italic>Bnip3<\/jats:italic>\n                      and\n                      <jats:italic>Nfkbia<\/jats:italic>\n                      . The overall content of ubiquitinated proteins was modestly but significantly lower in TA muscles from OVX\u2009+\u2009E2 vs. OVX mice (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.039). There were no differences between skmER\u03b1KO and skmER\u03b1WT mice or between skmER\u03b1KO\/OVX and skmER\u03b1KO\/OVX\u2009+\u2009E2 mice for any genes assessed, indicating that ER\u03b1 is required for E2 regulation of those genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results suggest that a mechanism whereby E2 protects against losses of skeletal muscle mass and strength is regulation of ubiquitin\u2010proteasomal mediators.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13698","type":"journal-article","created":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T06:05:37Z","timestamp":1737785137000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Transcriptomic Profiling Reveals 17\u03b2\u2010Estradiol Treatment Represses Ubiquitin\u2010Proteasomal Mediators in Skeletal Muscle of Ovariectomized Mice"],"prefix":"10.1002","volume":"16","author":[{"given":"Georgios","family":"Kararigas","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine University of Iceland  Reykjavik Iceland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara\u00a0C.","family":"Ebeling","sequence":"additional","affiliation":[{"name":"Division of Physical Therapy and Rehabilitation Science, Department of Family Medicine and Community Health University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengyun","family":"Le","sequence":"additional","affiliation":[{"name":"Division of Physical Therapy and Rehabilitation Science, Department of Family Medicine and Community Health University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaojuan","family":"Lai","sequence":"additional","affiliation":[{"name":"Division of Physical Therapy and Rehabilitation Science, Department of Family Medicine and Community Health University of Minnesota  Minneapolis Minnesota USA"},{"name":"College of Basic Medicine Guizhou University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmei","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, State Key Laboratory of Vascular Homeostasis and Remodeling, School of Basic Medical Sciences Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, State Key Laboratory of Vascular Homeostasis and Remodeling, School of Basic Medical Sciences Peking University  Beijing China"},{"name":"School of Sports Medicine Wuhan Institute of Physical Education  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5784-9289","authenticated-orcid":false,"given":"Dawn\u00a0A.","family":"Lowe","sequence":"additional","affiliation":[{"name":"Division of Physical Therapy and Rehabilitation Science, Department of Family Medicine and Community Health University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0000000000001612"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00361547"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glp082"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.91518.2008"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0840095"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.250092"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2019.03.033"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.277.2.H467"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.06.025"},{"key":"e_1_2_8_11_1","first-page":"7362","article-title":"Natural Aging and Ovariectomy Induces Parallel Phosphoproteomic Alterations in Skeletal Muscle of Female Mice","volume":"15","author":"Peyton M. 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