{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:54:27Z","timestamp":1779353667199,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2022S1A5A2A03055843"],"award-info":[{"award-number":["2022S1A5A2A03055843"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2025,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n The cellular prion protein (PrP\n C<\/jats:sup>\n ), a glycoprotein encoded by the\n PRNP<\/jats:italic>\n gene, is known to modulate muscle mass and exercise capacity. However, the role of PrP\n C<\/jats:sup>\n in the maintenance and regeneration of skeletal muscle during ageing remains unclear.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n This study investigated the change in PrP\n C<\/jats:sup>\n expression during muscle formation using C2C12 cells and evaluated muscle function in\n Prnp<\/jats:italic>\n wild\u2010type (WT) and knock\u2010out (KO) mice at different ages (1, 9 and 15\u2009months). To determine the role of PrP\n C<\/jats:sup>\n in skeletal muscle homeostasis during ageing, we conducted regeneration experiments via cardiotoxin injection in\n Prnp<\/jats:italic>\n mice to assess the effects of PrP\n C<\/jats:sup>\n deficiency on the senescence of satellite stem cells (SCs) and regenerative capacity in skeletal muscle.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Our data demonstrate that PrP\n C<\/jats:sup>\n expression increased significantly during muscle differentiation (\n p<\/jats:italic>\n \u2009<\u20090.01), correlating with myogenin (immunofluorescence at the differentiation stage). PrP\n C<\/jats:sup>\n deficiency disrupted muscle homeostasis, leading to age\u2010associated mitochondrial autophagy (Pink\u20101, +180%,\n p<\/jats:italic>\n \u2009<\u20090.001; Parkin, +161%,\n p<\/jats:italic>\n \u2009<\u20090.01) and endoplasmic reticulum stress (SERCA, \u221226%,\n p<\/jats:italic>\n \u2009<\u20090.05; IRE1\n \u03b1<\/jats:italic>\n , +195%,\n p<\/jats:italic>\n \u2009<\u20090.001) while decreasing the level of mitochondrial biogenesis (SIRT\u20101, \u221250%,\n p<\/jats:italic>\n \u2009<\u20090.01; PGC\u20101\n \u03b1<\/jats:italic>\n , \u221236%,\n p<\/jats:italic>\n \u2009<\u20090.05; VDAC, \u221227%,\n p<\/jats:italic>\n \u2009<\u20090.001), and activated oxidative stress (serum myoglobin, +23%,\n p<\/jats:italic>\n \u2009<\u20090.001; MDA, +23%,\n p<\/jats:italic>\n \u2009<\u20090.05; NF\n \u03ba<\/jats:italic>\n B, +117%, p\u2009<\u20090.05) during ageing, which accelerated reduced muscle growth or mass accumulation (tibialis anterior muscle mass, \u221223%,\n p<\/jats:italic>\n \u2009<\u20090.001; gastrocnemius muscle mass, \u221230%,\n p<\/jats:italic>\n \u2009<\u20090.001; muscle fibre size, \u221248%,\n p<\/jats:italic>\n \u2009<\u20090.05; MSTN, +160%,\n p<\/jats:italic>\n \u2009<\u20090.01; MAFbx, +83%,\n p<\/jats:italic>\n \u2009<\u20090.05). Furthermore, PrP\n C<\/jats:sup>\n deficiency induced the senescence (\n \u03b2<\/jats:italic>\n \u2010galactosidase, +60%,\n p<\/jats:italic>\n \u2009<\u20090.05; p16, +103%,\n p<\/jats:italic>\n \u2009<\u20090.001) of SCs, which was directly related to the defect in muscle recovery, with the senescence\u2010mediated enhancement of adipogenesis (PPAR\n \u03b3<\/jats:italic>\n , +74%,\n p<\/jats:italic>\n \u2009<\u20090.05) during the regeneration process after cardiotoxin\u2010induced muscle injury.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our findings demonstrate that PrP\n C<\/jats:sup>\n is indispensable for maintaining skeletal muscle homeostasis during ageing by modulating the functional integrity of mitochondria, ER and SCs.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcsm.13706","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T05:01:53Z","timestamp":1738040513000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["PrP\n C<\/sup>\n Glycoprotein Is Indispensable for Maintenance of Skeletal Muscle Homeostasis During Aging"],"prefix":"10.1002","volume":"16","author":[{"given":"Wenduo","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Sports Science, College of Natural Science Jeonbuk National University Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thi\u00a0Thu\u00a0Trang","family":"Kieu","sequence":"additional","affiliation":[{"name":"Department of Bioactive Material Sciences, Research Center of Bioactive Materials Jeonbuk National University Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zilin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Sports Science, College of Natural Science Jeonbuk National University Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun\u2010Jaung","family":"Sim","sequence":"additional","affiliation":[{"name":"Department of Bioactive Material Sciences, Research Center of Bioactive Materials Jeonbuk National University Jeonju Republic of Korea"},{"name":"Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences and School of Dentistry Jeonbuk National University Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seohyeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences University of California Berkeley Berkeley California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong\u2010Chae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Bioactive Material Sciences, Research Center of Bioactive Materials Jeonbuk National University Jeonju Republic of Korea"},{"name":"Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences and School of Dentistry Jeonbuk National University Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoonjung","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory of Integrated Physiology, Department of Health & Human Performance University of Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang\u00a0Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Sports Science, College of Natural Science Jeonbuk National University Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1879-5927","authenticated-orcid":false,"given":"Sung\u2010Ho","family":"Kook","sequence":"additional","affiliation":[{"name":"Department of Bioactive Material Sciences, Research Center of Bioactive Materials Jeonbuk National University Jeonju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.123935"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-023-00805-8"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-014-9915-y"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/84.3.475"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04369-z"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13694-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2021.111544"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2016.02.002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.793088"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/58.11.M999"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-818698-5.00009-2"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2016.11.006"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0148607108324585"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature16187"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2241"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12238"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608885104"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43826-7"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01040-09"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.24708"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2007.04.004"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2005.06.033"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-9335(98)80043-5"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.3945"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-020-03624-6"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1633"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/356577a0"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2012.91"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2021.02.025"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.4196\/kjpp.2018.22.4.427"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2015.10.014"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2022.101440"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S234665"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1016-8478(23)17608-4"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2023.0713"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.63.277"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64250-2"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-013-2800-y"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/82_2010_78"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-007-0588-3"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13706","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcsm.13706","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcsm.13706","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:31:25Z","timestamp":1775979085000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcsm.13706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,28]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/jcsm.13706"],"URL":"https:\/\/doi.org\/10.1002\/jcsm.13706","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,28]]},"assertion":[{"value":"2024-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13706"}}