{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T16:58:06Z","timestamp":1788973086541,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sarcopenia, the age\u2010related decline in muscle mass and muscle strength, significantly contributes to falls, diminished quality of life, and mortality. Although mitochondrial dysfunction is increasingly implicated in sarcopenia, the underlying mechanisms are not fully discovered. Low\u2010magnitude high\u2010frequency vibration (LMHFV), a recommended treatment by the Centers for Disease Control and Prevention (CDC) to reduce fall risk, remains poorly understood of the mechanism on improving skeletal muscle quality. This study aims to investigate whether mitochondrial dysfunction contributes to sarcopenia and evaluate whether LMHFV mitigates sarcopenia by improving mitochondrial homeostasis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The relationship between mitochondria dysfunction and sarcopenia using senescence accelerated mice prone 8 (SAMP8) model was investigated, assessing muscle and mitochondria. The effects of LMHFV on muscle and mitochondria were evaluated in SAMP8 mice during sarcopenia progression. The role of miR\u2010378 in muscle and mitochondrial homeostasis were evaluated in SAMP8 mice and transgenic over\u2010expressing miR\u2010378 mice (TG mice). The target gene of miR\u2010378 was investigated by dual\u2010luciferase reporter assay in C2C12 cells. Subsequently, we evaluated the effect of LMHFV on miR\u2010378 using both mouse models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Reduction in muscle strength was observed from the ages of month 8 to 10 in SAMP8 mice (grip strength decreased 27.1%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0263; twitch force decreased 29.1%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0178; tetanic force decreased 29.9%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.011), as well as muscle atrophy (cross\u2010section area: 38.3%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0121). Mitochondrial morphological deterioration was noticed from month 6 to 10. Mitochondrial homeostasis, including biogenesis, fusion, fission, mitophagy, and ATP production declined from month 6 to 10. Compared to control group at month 10, knocking down miR\u2010378 in SAMP8 mice mitigated sarcopenia (twitch force increased 44.3%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0023; tetanic force increased 51.9%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0005), improved mitochondrial morphologies (mitochondrial number increased 1.65\u2010fold,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0023; mitochondrial density increased 1.65\u2010fold,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0023; mitochondrial relative area increased 9.05\u2010fold,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0019) along with improved mitochondrial homeostasis. Over\u2010expressing miR\u2010378 in transgenic mice exacerbated muscle atrophy and mitochondrial deterioration significantly. The dual\u2010luciferase reporter assay in C2C12 cells revealed that miR\u2010378 inhibited PGC\u20101\u03b1 directivity. LMHFV was found to mitigate sarcopenia by modulating mitochondrial homeostasis, such as attenuating mitochondrial morphological deterioration and improving mitochondrial biogenesis through increasing PGC\u20101\u03b1 via inhibiting miR\u2010378 in skeletal muscle.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings indicate that mitochondrial biogenesis, fusion, fission, and mitophagy were compromised during progression of sarcopenia, with mitochondrial deterioration preceding the onset of sarcopenia symptoms. The study also demonstrated that LMHFV could attenuate sarcopenia by modulating mitochondrial quality control through inhibiting miR\u2010378, highlighting its therapeutic potential in the management of age\u2010related muscular degeneration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13740","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T20:15:41Z","timestamp":1739996141000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Low\u2010Magnitude High\u2010Frequency Vibration Attenuates Sarcopenia by Modulating Mitochondrial Quality Control via Inhibiting miR\u2010378"],"prefix":"10.1002","volume":"16","author":[{"given":"Yu\u2010Feng","family":"Long","sequence":"first","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong  Hong Kong China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Hong Kong SAR China"},{"name":"Department of Spine Surgery The 6th Affiliated Hospital of Shenzhen University Health Science Center  Shenzhen China"},{"name":"Translational Medicine R&amp;D Center, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Cui","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong  Hong Kong China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianjin","family":"Wang","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese 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University Hospital Mannheim University of Heidelberg  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinton","family":"Rubin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Stony Brook University  Stony Brook New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Li","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong  Hong Kong China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Qin","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong  Hong Kong China"},{"name":"Li Ka Shing Institute of Health Sciences The Chinese 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