{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:01:20Z","timestamp":1789567280322,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2022YFC3602201"],"award-info":[{"award-number":["2022YFC3602201"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cisplatin is a widely used chemotherapeutic agent for treating solid tumours. Still, it induces severe side effects, including muscle atrophy. Understanding the mechanisms of cisplatin\u2010induced muscle loss and exploring potential therapeutic strategies are essential. Parvalbumin (PV) interneurons in the striatum play a crucial role in motor control, and recent studies suggest that their activation may alleviate motor deficits. This study investigates the effects of chemogenetic activation of PV interneurons on cisplatin\u2010induced muscle atrophy and motor dysfunction in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Wild\u2010type C57BL\/6 mice and transgenic hM3Dq mice were used in this study. Cisplatin (3\u2009mg\/kg) was administered intraperitoneally for 7\u2009days to induce muscle atrophy. Mice were then treated with clozapine\u2010n\u2010oxide (CNO) to activate PV interneurons. Muscle strength and endurance were assessed using grip strength measurements, the inverted grid test and the wire hang test. Neuromuscular junction (NMJ) integrity was examined via histological analysis. Exercise intervention was also included, using a treadmill with a 15\u00b0 incline for 60\u2009min at varying speeds during seven consecutively days.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Cisplatin treatment significantly reduced body weight (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), grip strength (forelimb strength:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001, four\u2010limb strength:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), endurance (inverted grid test:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.047, wire hang test:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.014) and NMJ integrity (partially innervated NMJs:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0383). PV interneuron activation with CNO improved spontaneous motor activity in cisplatin\u2010treated mice, as evidenced by a significant increase in total travel distance (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.049) in the open\u2010field test. Histological analysis showed a reduced ratio of partially innervated NMJs in the PV\u2010cre group compared to the control virus group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0441). Muscle strength also improved significantly, with forelimb grip strength increased (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and four\u2010limb grip strength increased (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.018). Muscle wet\u2010weight ratios were significantly higher in the PV\u2010cre group (quadriceps:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.030). Exercise intervention significantly improved grip strength (forelimb:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001, four\u2010limb:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002), muscle endurance (four\u2010limb hang test:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.048) and muscle weight (quadriceps:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.015, gastrocnemius:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.022), with an increase in muscle fibre cross\u2010sectional area (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0018).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Activation of PV interneurons significantly alleviates cisplatin\u2010induced motor deficits and muscle atrophy by improving spontaneous motor activity, NMJ integrity and muscle function. It has a similar effect to short\u2010term exercise and may offer a promising therapeutic strategy for mitigating chemotherapy\u2010induced muscle atrophy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13782","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T08:14:46Z","timestamp":1744100086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Activating Striatal Parvalbumin Interneurons to Alleviate Chemotherapy\u2010Induced Muscle Atrophy"],"prefix":"10.1002","volume":"16","author":[{"given":"Jun","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Pain, Huadong Hospital Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Clinical Geriatric Medicine Fudan University  Shanghai China"},{"name":"School of Exercise and Health Shanghai University of Sport  Shanghai China"},{"name":"Department of Rehabilitation Medicine Shanghai University of Medicine &amp; Health Sciences Affiliated Zhoupu Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pain, Huadong Hospital Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Clinical Geriatric Medicine Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pain, Huadong Hospital Fudan University  Shanghai China"},{"name":"School of Exercise and Health Shanghai University of Sport  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyu","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Pain, Huadong Hospital Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Clinical Geriatric Medicine Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junlong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pain, Huadong Hospital Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Clinical Geriatric Medicine Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6554-1690","authenticated-orcid":false,"given":"Yongjun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pain, Huadong Hospital Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Clinical Geriatric Medicine Fudan University  Shanghai 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