{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T03:42:07Z","timestamp":1781235727880,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81801392"],"award-info":[{"award-number":["81801392"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2025,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n The Popeye domain containing 3 (POPDC3) protein is essential for the maintenance of skeletal muscle homeostasis. POPDC3 is a pathogenic variant gene of limb\u2010girdle muscular dystrophy (LGMD), and its variants lead to LGMDR26. At the animal level, zebrafish larvae with\n popdc3<\/jats:italic>\n mutations develop tail curls and muscle atrophy. However, the mechanism of skeletal muscle atrophy induced by\n POPDC3<\/jats:italic>\n variants\/loss remains unclear.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Eight\u2010month\u2010old male WT and\n popdc3<\/jats:italic>\n mKO zebrafish were used for this research. Loli Track (Denwmark) and Loligo Swimming Respirometer were used to observe the zebrafish's swimming ability. The zebrafish skeletal muscle structure and cross\u2010sectional area (CSA) were observed and counted by transmission electron microscopy (TEM), H&E and wheat germ agglutinin (WGA). Enriched genes and signalling pathways were analysed using RNA sequencing, and the effects of\n popdc3<\/jats:italic>\n mKO on zebrafish skeletal muscle mitochondrial respiration, biogenesis and dynamics were examined to investigate possible mechanisms.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The swimming ability of\n popdc3<\/jats:italic>\n mKO zebrafish was reduced, and as evidenced by the reluctance to move, fewer movement trajectories, the total distance travelled (\n p<\/jats:italic>\n \u2009<\u20090.001), the average velocity of movement (\n p<\/jats:italic>\n \u2009<\u20090.001), oxygen consumption (MO\n 2<\/jats:sub>\n ) (\n p<\/jats:italic>\n \u2009<\u20090.01), maximum oxygen consumption (MO\n 2max<\/jats:sub>\n ) (\n p<\/jats:italic>\n \u2009<\u20090.05), critical swimming speed (U\n crit<\/jats:sub>\n ) (\n p<\/jats:italic>\n \u2009<\u20090.01) and relative swimming speed (U\n crit\u2010r<\/jats:sub>\n ) (\n p<\/jats:italic>\n \u2009<\u20090.01) were significantly decreased and increased of the exhaustive swimming time (\n p<\/jats:italic>\n \u2009<\u20090.01). In addition, loss of\n popdc3<\/jats:italic>\n reduced zebrafish skeletal muscle weight (\n p<\/jats:italic>\n \u2009<\u20090.001), muscle\/body weight (\n p<\/jats:italic>\n \u2009<\u20090.01), myofibre size and CSA (\n p<\/jats:italic>\n \u2009<\u20090.01), increased protein degradation (ubiquitination and autophagy) (\n p<\/jats:italic>\n \u2009<\u20090.05) and decreased protein synthesis (\n p<\/jats:italic>\n \u2009<\u20090.05), suggesting that\n popdc3<\/jats:italic>\n deficiency induces zebrafish skeletal muscle atrophy. Further,\n popdc3<\/jats:italic>\n mKO zebrafish mitochondrial function is reduced, as evidenced by impaired mitochondrial respiration, decreased biogenesis and kinetic imbalance (\n p<\/jats:italic>\n \u2009<\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n POPDC3, a Popeye protein, plays an important role in controlling mitochondrial function and skeletal muscle mass and strength. Loss of\n popdc3<\/jats:italic>\n decreases mitochondrial respiration and mitochondrial biogenesis, disrupting kinetic homeostasis, which induces mitochondrial dysfunction and impaired protein turnover (reduced synthesis and increased degradation), leading to zebrafish skeletal muscle atrophy.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcsm.13794","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:36:24Z","timestamp":1745462184000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Loss of\n popdc3<\/i>\n Impairs Mitochondrial Function and Causes Skeletal Muscle Atrophy and Reduced Swimming Ability in Zebrafish"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5784-3365","authenticated-orcid":false,"given":"Chen\u2010Chen","family":"Sun","sequence":"first","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation, College of Physical Education Hunan Normal University Changsha Hunan China"},{"name":"Institute of Physical Education Hunan First Normal University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang\u2010Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation, College of Physical Education Hunan Normal University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation, College of Physical Education Hunan Normal University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang\u2010Ling","family":"Xiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation, College of Physical Education Hunan Normal University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010Tao","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation, College of Physical Education Hunan Normal University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi\u2010Yang","family":"Peng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and Exercise Rehabilitation, College of Physical Education Hunan Normal University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu\u2010Shan","family":"Wu","sequence":"additional","affiliation":[{"name":"Center for Heart Development, College of Life Sciences Hunan Normal University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Fa","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Physical Fitness and 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