{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T04:02:18Z","timestamp":1776052938641,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T00:00:00Z","timestamp":1745107200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["2021R1C1C2009548"],"award-info":[{"award-number":["2021R1C1C2009548"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["2023R1A2C1004702"],"award-info":[{"award-number":["2023R1A2C1004702"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Physical exercise has beneficial effects on various organs, including skeletal muscle. However, not all patients are capable of engaging in exercise to maintain muscle function, which underscores the importance of identifying molecular mechanisms of physical training that could lead to the discovery of exercise\u2010mimicking molecules.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study sought to identify molecular mediators of exercise that could improve muscle function. We focused on the exercise\u2010induced microRNA (miR)\u2010129\u20103p, investigating its role and effects on mitochondrial activity both in\u00a0vivo and in\u00a0vitro. The expression of miR\u2010129\u20103p was analysed in skeletal muscle following exercise, and its downstream effects on the poly (ADP\u2010ribose) polymerase\u20101 (Parp1)\u2010SIRT1\u2010PGC1\u03b1 signalling pathway were elucidated. Functional studies were conducted using muscle\u2010specific overexpression of miR\u2010129\u20103p in adult mice and intramuscular injection of AAV9\u2010miR\u2010129\u20103p in obese mice to assess exercise capacity and muscle strength.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Exercise was found to upregulate miR\u2010129\u20103p in skeletal muscle (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), which directly inhibits\n                      <jats:italic>Parp1<\/jats:italic>\n                      , a major NAD\n                      <jats:sup>+<\/jats:sup>\n                      \u2010consuming enzyme. This inhibition leads to increased NAD\n                      <jats:sup>+<\/jats:sup>\n                      levels (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), activating SIRT1 and subsequently reducing the acetylation of PGC1\u03b1, thereby enhancing mitochondrial function. Muscle\u2010specific overexpression of miR\u2010129\u20103p in adult mice significantly enhanced exercise capacity (&gt;\u2009130%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001), while AAV9\u2010miR\u2010129\u20103p injections ameliorated muscle weakness (twitch force, &gt;\u2009140%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05; tetanic force, &gt;\u2009160%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01) in obese mice. In human skeletal muscle myoblasts, miR\u2010129\u20103p improved mitochondrial function via the PARP1\u2010SIRT1\u2010PGC1\u03b1 signalling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings suggest that miR\u2010129\u20103p, induced by exercise, can mimic the beneficial effects of physical exercise. This highlights miR\u2010129\u20103p as a potential therapeutic target for improving muscle health, especially in individuals unable to exercise.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13823","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T02:03:49Z","timestamp":1745201029000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["ExermiR\u2010129\u20103p Enhances Muscle Function by Improving Mitochondrial Activity Through PARP1 Inhibition"],"prefix":"10.1002","volume":"16","author":[{"given":"Yeo\u00a0Jin","family":"Shin","sequence":"first","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae\u00a0Won","family":"Yang","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"},{"name":"Department of Bioscience KRIBB School, Korea University of Science and Technology (UST)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heeyeon","family":"Jeong","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"},{"name":"Department of Bioscience KRIBB School, Korea University of Science and Technology (UST)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"},{"name":"Department of Bioinformatics KRIBB School, Korea University of Science and Technology (UST)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bora","family":"Lee","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung\u2010Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju\u00a0Yeon","family":"Kwak","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"},{"name":"Department of Medical Science Chungnam National University College of Medicine  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u00a0Hoon","family":"Son","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"},{"name":"Biohybrid Systems Group, Coulter Department of Biomedical Engineering Georgia Institute of Technology &amp; Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kap\u00a0Jung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Eulji University College of Medicine  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u00a0Ryoul","family":"Yang","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"},{"name":"Department of Bioscience KRIBB School, Korea University of Science and Technology (UST)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuna","family":"Kim","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"},{"name":"Department of Bioinformatics KRIBB School, Korea University of Science and Technology (UST)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki\u2010Sun","family":"Kwon","sequence":"additional","affiliation":[{"name":"Aging Convergence Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Daejeon Republic of Korea"},{"name":"Aventi Inc.  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