{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:23:05Z","timestamp":1785363785935,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T00:00:00Z","timestamp":1745193600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001605"],"award-info":[{"award-number":["82001605"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2023YFC2706300"],"award-info":[{"award-number":["2023YFC2706300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Maternal prepregnancy obesity predisposes offspring to obesity and metabolic disorders, yet its impact on skeletal muscle fibre transition remains unclear. Given that skeletal muscle plays a crucial role in systemic metabolism, we investigated how maternal prepregnancy high\u2010fat diet (HFD) influences muscle fibre composition and metabolic function in offspring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We established mouse models with a prepregnancy chow diet (CD) and a prepregnancy high\u2010fat diet (HFD) for 8\u2009weeks to compare metabolic phenotypes in offspring. Skeletal muscles from offspring were analysed using RNA sequencing, quantitative reverse transcription polymerase chain reaction and western blot to understand the changes in metabolic and signalling pathways. siRNA knockdown and lentiviral\u2010mediated overexpression experiments were conducted in\u00a0vitro and in\u00a0vivo to validate molecular mechanisms. Chromatin immunoprecipitation followed by qPCR (ChIP\u2010qPCR) was used to assess histone modification levels at promoter regions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Male and female offspring of prepregnancy obese dams (mHFD) exhibited a significant reduction in slow\u2010twitch oxidative fibres (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and an increase in fast\u2010twitch glycolytic fibres compared with controls. This was accompanied by impaired glucose tolerance (AUC increased by 12.87%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), insulin resistance and mitochondrial dysfunction (mtDNA copy number reduced by 31%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). RNA sequencing identified IDH2 as the most significantly downregulated gene (29.67% decrease,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), with protein levels further reduced in male (30.15%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01) and female (46.02%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001) offspring. IDH2 knockdown in C2C12 cells impaired mitochondrial biogenesis and led to higher oxidative stress (NADP+\/NADPH ratio elevated by 32%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), while IDH2 overexpression restored mitochondrial integrity, enhanced slow\u2010twitch fibre proportion (26.43\u2009\u00b1\u20090.6936% in mHFD\u2010LV\u2010IDH2,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01) and improved glucose metabolism (fasting glucose reduced by 14.7%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). ChIP\u2010qPCR revealed increased H3K9me3 enrichment at the IDH2 promoter (2.54\u2010fold in males, 2.55\u2010fold in females,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001), suggesting transgenerational epigenetic regulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Maternal prepregnancy obesity induces a metabolic shift in offspring skeletal muscle by promoting a slow\u2010to\u2010fast fibre transition and impairing mitochondrial biogenesis. This effect is mediated by IDH2 suppression via H3K9me3 histone modification, contributing to systemic insulin resistance. Targeting IDH2 may represent a potential therapeutic strategy to mitigate metabolic dysfunction in offspring exposed to maternal prepregnancy obesity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.13825","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T06:03:22Z","timestamp":1745215402000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prepregnancy Obesity Reprograms Offspring Skeletal Muscle Fibre Transition Through H3K9me3"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9201-210X","authenticated-orcid":false,"given":"Yichi","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pediatrics Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"},{"name":"Hubei Key Laboratory of Pediatric Genetic Metabolic and Endocrine Rare Diseases  Wuhan 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Wuhan Hubei China"},{"name":"Hubei Key Laboratory of Pediatric Genetic Metabolic and Endocrine Rare Diseases  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"},{"name":"Hubei Key Laboratory of Pediatric Genetic Metabolic and Endocrine Rare Diseases  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"},{"name":"Hubei Key Laboratory of Pediatric Genetic Metabolic and Endocrine Rare Diseases  Wuhan 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