{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T01:14:54Z","timestamp":1777338894730,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sarcopenia, a significant geriatric syndrome, faces challenges in accurate diagnosis due to limitations of current imaging techniques. This study explores the novel application of multispectral optoacoustic tomography (MSOT) in evaluating sarcopenia, focusing on quantifying oxygen dynamics and collagen distribution in skeletal muscles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted MSOT imaging on the lower limbs of senescence\u2010accelerated mouse prone 8 (SAMP8;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914) and senescence\u2010accelerated mouse resistant 1 (SAMR1;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098) models, using light wavelengths of 760, 840 and 930\u2009nm. CT, histopathology and immunofluorescence were used for cross\u2010validation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Label\u2010free MSOT imaging directly visualized muscle structure and metabolism with high spatiotemporal resolution. Compared to SAMR1 controls, sarcopenic SAMP8 mice demonstrated 23.8% lower HbO\n                      <jats:sub>2<\/jats:sub>\n                      levels (SAMP8: 0.0016\u2009\u00b1\u20090.0003\u2009a.u. vs. SAMR1: 0.0021\u2009\u00b1\u20090.0005\u2009a.u.;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.018) and reduced metabolic activity in skeletal muscles. SAMP8 mice also revealed 43.2% higher collagen content (SAMP8: 3.451\u2009\u00b1\u20091.159\u2009a.u. vs. SAMR1: 2.409\u2009\u00b1\u20090.635\u2009a.u.;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.030) alongside more disordered muscle structure, suggesting increased fibrosis. An inverse correlation was observed between computed tomography (CT) values and MSOT\u2010derived collagen signals (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u22120.789,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), whereas no such correlation existed with HbO\n                      <jats:sub>2<\/jats:sub>\n                      , indicating that MSOT provides unique metabolic insights beyond traditional imaging techniques.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This first application of MSOT in sarcopenia research highlights its potential as a noninvasive, real\u2010time tool for early diagnosis, therapeutic evaluation and mechanistic understanding. Its ability to detect metabolic changes not captured by CT underscores its complementary role in comprehensive muscle assessment. Future research should focus on longitudinal studies and clinical translation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcsm.70088","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T18:15:07Z","timestamp":1761070507000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multispectral Optoacoustic Tomography of Skeletal Muscle Unveils Microcirculation and Oxygen Metabolism Alterations in Sarcopenia"],"prefix":"10.1002","volume":"16","author":[{"given":"Ying","family":"Yang","sequence":"first","affiliation":[{"name":"School of Computer Science and Technology Chongqing University of Posts and Telecommunications  Chongqing China"},{"name":"Geriatric Diseases Institute of Chengdu\/Cancer Prevention and Treatment Institute of Chengdu, Department of Neurology Chengdu Fifth People's Hospital  Chengdu 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Bi","sequence":"additional","affiliation":[{"name":"School of Computer Science and Technology Chongqing University of Posts and Telecommunications  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Computer Science and Technology Chongqing University of Posts and Telecommunications  Chongqing China"},{"name":"Department of Ultrasound Medicine Chengdu Fifth People's Hospital  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Computer Science and Technology Chongqing University of Posts and Telecommunications  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanting","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Computer Science and Technology Chongqing University of Posts and Telecommunications  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shixie","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of Florida College of Medicine  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiran","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Department Chengdu Fifth People's Hospital  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tangming","family":"Peng","sequence":"additional","affiliation":[{"name":"Geriatric Diseases Institute of Chengdu\/Cancer Prevention and Treatment Institute of Chengdu, Department of Neurology Chengdu Fifth People's Hospital  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Li","sequence":"additional","affiliation":[{"name":"Geriatric Diseases 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